Abstract：
:An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. Although biochemical studies indicate that N(6)-methyladenosine (m(6)A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and functions has been impeded by a lack of robust analytical methods. Here we present the human and mouse m(6)A modification landscape in a transcriptome-wide manner, using a novel approach, m(6)A-seq, based on antibody-mediated capture and massively parallel sequencing. We identify over 12,000 m(6)A sites characterized by a typical consensus in the transcripts of more than 7,000 human genes. Sites preferentially appear in two distinct landmarks--around stop codons and within long internal exons--and are highly conserved between human and mouse. Although most sites are well preserved across normal and cancerous tissues and in response to various stimuli, a subset of stimulus-dependent, dynamically modulated sites is identified. Silencing the m(6)A methyltransferase significantly affects gene expression and alternative splicing patterns, resulting in modulation of the p53 (also known as TP53) signalling pathway and apoptosis. Our findings therefore suggest that RNA decoration by m(6)A has a fundamental role in regulation of gene expression.
journal_name
Naturejournal_title
Natureauthors
Dominissini D,Moshitch-Moshkovitz S,Schwartz S,Salmon-Divon M,Ungar L,Osenberg S,Cesarkas K,Jacob-Hirsch J,Amariglio N,Kupiec M,Sorek R,Rechavi Gdoi
10.1038/nature11112subject
Has Abstractpub_date
2012-04-29 00:00:00pages
201-6issue
7397eissn
0028-0836issn
1476-4687pii
nature11112journal_volume
485pub_type
杂志文章相关文献
NATURE文献大全abstract：:Transgenic mice bearing the cellular myc oncogene coupled to the immunoglobulin mu or kappa enhancer frequently develop a fatal lymphoma within a few months of birth. Since the tumours represent represent both immature and mature B lymphocytes, constitutive c-myc expression appears to be highly leukaemogenic at severa...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/318533a0
更新日期：1985-12-12 00:00:00
abstract：:The Gram-negative bacterium Yersinia pestis is the causative agent of the systemic invasive infectious disease classically referred to as plague, and has been responsible for three human pandemics: the Justinian plague (sixth to eighth centuries), the Black Death (fourteenth to nineteenth centuries) and modern plague ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35097083
更新日期：2001-10-04 00:00:00
abstract：:Cellular ras genes encode a family of membrane-associated proteins (p21ras) that bind guanine nucleotide and possess a low intrinsic GTPase activity. The p21ras proteins are ubiquitously expressed in mammalian cells and are thought to be involved in a growth-promoting signal transduction pathway; their mode of action,...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/347396a0
更新日期：1990-09-27 00:00:00
abstract：:The Suzuki-Miyaura cross-coupling of organoboron nucleophiles with aryl halide electrophiles is one of the most widely used carbon-carbon bond-forming reactions in organic and medicinal chemistry1,2. A key challenge associated with these transformations is that they generally require the addition of an exogenous base,...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0628-7
更新日期：2018-11-01 00:00:00
abstract：:Deception is common in nature and humans are no exception. Modern societies have created institutions to control cheating, but many situations remain where only intrinsic honesty keeps people from cheating and violating rules. Psychological, sociological and economic theories suggest causal pathways to explain how the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature17160
更新日期：2016-03-24 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 新闻
doi：10.1038/d41586-018-06989-8
更新日期：2018-10-01 00:00:00
abstract：:We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The centromeres are between 35 and 110 kilobases (kb) and contain related repeats including a highly conserved 1.8-kb element. Regions...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature724
更新日期：2002-02-21 00:00:00
abstract：:Suppressor of cytokine signalling-2 (SOCS-2) is a member of the suppressor of cytokine signalling family, a group of related proteins implicated in the negative regulation of cytokine action through inhibition of the Janus kinase (JAK) signal transducers and activators of transcription (STAT) signal-transduction pathw...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35016611
更新日期：2000-06-29 00:00:00
abstract：:The taxonomy of a parasite can be an important guide to its pathogenic characteristics. A wide range of anatomical, biochemical and behavioural tests is now being developed to define different strains and subspecies of the main tropical parasites. ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/273600a0
更新日期：1978-06-22 00:00:00
abstract：:Activation of Janus kinase 2 (JAK2) by chromosomal translocations or point mutations is a frequent event in haematological malignancies. JAK2 is a non-receptor tyrosine kinase that regulates several cellular processes by inducing cytoplasmic signalling cascades. Here we show that human JAK2 is present in the nucleus o...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08448
更新日期：2009-10-08 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 评论,新闻
doi：10.1038/d41586-018-07328-7
更新日期：2018-11-01 00:00:00
abstract：:Finite element analysis (FEA) is used by industrial designers and biomechanicists to estimate the performance of engineered structures or human skeletal and soft tissues subjected to varying regimes of stress and strain. FEA is rarely applied to problems of biomechanical design in animals, despite its potential to inf...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35059070
更新日期：2001-02-22 00:00:00
abstract：:Mesolimbic dopamine-releasing neurons appear to be important in the brain reward system. One behavioural paradigm that supports this hypothesis is intracranial self-stimulation (ICS), during which animals repeatedly press a lever to stimulate their own dopamine-releasing neurons electrically. Here we study dopamine re...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/18019
更新日期：1999-03-04 00:00:00
abstract：:Theoretical and empirical research highlights the role of punishment in promoting collaborative efforts. However, both the emergence and the stability of costly punishment are problematic issues. It is not clear how punishers can invade a society of defectors by social learning or natural selection, or how second-orde...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09203
更新日期：2010-08-12 00:00:00
abstract：:The germ-cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have previously demonstrated that, by using cytokines, embryonic stem cells and induced pluripotent stem cells can be induced into epiblast-like cells (EpiLCs) and then in...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12417
更新日期：2013-09-12 00:00:00
abstract：:A decade ago, Lovelock and Whitfield raised the question of how much longer the biosphere can survive on Earth. They pointed out that, despite the current fossil-fuel induced increase in the atmospheric CO2 concentration, the long-term trend should be in the opposite direction: as increased solar luminosity warms th...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/360721a0
更新日期：1992-12-24 00:00:00
abstract：:Various mechanisms have been proposed for beta-adrenergically mediated relaxation of smooth muscle. All theories suggest the involvement of cyclic AMP as a second messenger: beta-agonists stimulate adenylate cyclase which converts ATP to cyclic AMP and protein kinase, activated by cyclic AMP, is then thought to cataly...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/281682a0
更新日期：1979-10-25 00:00:00
abstract：:Signal sequences target proteins for secretion from cells or for integration into cell membranes. As nascent proteins emerge from the ribosome, signal sequences are recognized by the signal recognition particle (SRP), which subsequently associates with its receptor (SR). In this complex, the SRP and SR stimulate each ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02250
更新日期：2004-01-15 00:00:00
abstract：:Chromatin reorganization is governed by multiple post-translational modifications of chromosomal proteins and DNA. These histone modifications are reversible, dynamic events that can regulate DNA-driven cellular processes. However, the molecular mechanisms that coordinate histone modification patterns remain largely u...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10656
更新日期：2011-11-27 00:00:00
abstract：:Groundwater drawn daily from shallow alluvial sands by millions of wells over large areas of south and southeast Asia exposes an estimated population of over a hundred million people to toxic levels of arsenic. Holocene aquifers are the source of widespread arsenic poisoning across the region. In contrast, Pleistocene...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12444
更新日期：2013-09-12 00:00:00
abstract：:General alarm about genetics continues, fanned by the expectation that what may one day be possible will be a reality tomorrow. Moderation is the most urgent need, but understanding would also help. ...
journal_title：Nature
pub_type： 社论
doi：10.1038/367099a0
更新日期：1994-01-13 00:00:00
abstract：:Deep neural networks have achieved impressive successes in fields ranging from object recognition to complex games such as Go1,2. Navigation, however, remains a substantial challenge for artificial agents, with deep neural networks trained by reinforcement learning3-5 failing to rival the proficiency of mammalian spat...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0102-6
更新日期：2018-05-01 00:00:00
abstract：:Experimental and simulation studies show that small monomeric proteins fold in one kinetic step, which entails overcoming the free-energy barrier between the unfolded and the native protein through a transition state. Two models of transition state formation have been proposed: a 'nonspecific' one in which it depends ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/379096a0
更新日期：1996-01-04 00:00:00
abstract：:MicroRNAs (miRNAs) are approximately 22-nucleotide endogenous RNAs that often repress the expression of complementary messenger RNAs. In animals, miRNAs derive from characteristic hairpins in primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the base of the stem to liberate a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05983
更新日期：2007-07-05 00:00:00
abstract：:Diatoms dominate the biomass of phytoplankton in nutrient-rich conditions and form the basis of some of the world's most productive marine food webs. The diatom nuclear genome contains genes with bacterial and plastid origins as well as genes of the secondary endosymbiotic host (the exosymbiont), yet little is known a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10074
更新日期：2011-05-12 00:00:00
abstract：:The tropical forests of Borneo and Amazonia may each contain more tree species diversity in half a square kilometre than do all the temperate forests of Europe, North America, and Asia combined. Biologists have long been fascinated by this disparity, using it to investigate potential drivers of biodiversity. Latitudin...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature24038
更新日期：2017-10-05 00:00:00
abstract：:Saturn's moon Titan has a nitrogen atmosphere comparable to Earth's, with a surface pressure of 1.4 bar. Numerical models reproduce the tropospheric conditions very well but have trouble explaining the observed middle-atmosphere temperatures, composition and winds. The top of the middle-atmosphere circulation has been...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11611
更新日期：2012-11-29 00:00:00
abstract：:7SL RNA is an abundant cytoplasmic RNA which functions in protein secretion as a component of the signal recognition particle. Alu sequences are the most abundant family of human and rodent middle repetitive DNA sequences (reviewed in ref. 2). The primary structure of human 7SL RNA consists of an Alu sequence interrup...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/312171a0
更新日期：1984-11-08 00:00:00
abstract：:At the molecular level, muscle contraction is the result of cyclic interaction between myosin crossbridges, which extend from the thick filament, and the thin filament, which consists mainly of actin. The energy for work done by a single crossbridge during a cycle of attachment, generation of force, shortening and det...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/355638a0
更新日期：1992-02-13 00:00:00
abstract：:The overexpression of defined transcription factors in somatic cells results in their reprogramming into induced pluripotent stem (iPS) cells. The extremely low efficiency and slow kinetics of in vitro reprogramming suggest that further rare events are required to generate iPS cells. The nature and identity of these e...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08285
更新日期：2009-08-27 00:00:00
