Abstract:
AIM:To investigate the effects of small interference RNA (siRNA) targeting of Cdx2 on human gastric cancer MGC-803 cells in vitro and in vivo. METHODS:The recombinant pSilencer 4.1-Cdx2 siRNA plasmids were constructed and transfected into gastric cancer MGC-803 cells in vitro. The stable transfectants were selected. The effects of Cdx2 siRNA on growth, proliferation, cell cycle, apoptosis, migration and invasiveness of human gastric cancer MGC-803 cells were evaluated and the expression of phosphatase and tensin homolog (PTEN), caspase-9 and caspase-3 was observed in vitro by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analysis. We also investigated the effect of Cdx2 siRNA on growth of MGC-803 cells in nude mice in vivo. RESULTS:Cdx2 siRNA led to inhibition of endogenous Cdx2 mRNA and protein expression as determined by RT-PCR and Western blotting analysis. Cdx2 siRNA significantly inhibited cell growth and proliferation, blocked entry into the S-phase of the cell cycle, induced cell apoptosis, and reduced the motility and invasion of MGC-803 cells. Cdx2 siRNA also increased PTEN expression, and activated caspase-9 and caspase-3 in MGC-803 cells in vitro . In addition, siRNA targeting of Cdx2 inhibited the growth of MGC-803 cells and promoted tumor cell apoptosis in vivo in nude mice tumor models. CONCLUSION:Cdx2 was involved in regulating pro-gression of human gastric cancer cells MGC-803. Manipulation of Cdx2 expression may be a potential therapeutic strategy for gastric cancer.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Wang XT,Xie YB,Xiao Qdoi
10.3748/wjg.v18.i16.1903subject
Has Abstractpub_date
2012-04-28 00:00:00pages
1903-14issue
16eissn
1007-9327issn
2219-2840journal_volume
18pub_type
杂志文章abstract::Cystic dystrophy of the duodenal wall is a rare condition characterized by the development of cysts in heterotopic pancreatic tissue localized in the duodenal wall. A 38-year-old man was admitted to the hospital for abdominal pain and vomiting after food intake. The diagnosis of acute pancreatitis was initially suspec...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v10.i17.2609
更新日期:2004-09-01 00:00:00
abstract::The combination of either boceprevir or telaprevir with ribavirin and interferon (triple therapy) has been shown to be more effective than ribavirin+interferon (dual therapy) for the treatment of genotype 1 hepatitis C. Since the benefit of these treatments takes place after years, simulation models are needed to pred...
journal_title:World journal of gastroenterology
pub_type: 信件
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journal_title:World journal of gastroenterology
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doi:10.3748/wjg.v11.i30.4628
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.2367
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:2005-02-21 00:00:00
abstract:AIM:To determine risk factors associated with hepatitis C virus (HCV) treatment failure after direct acting antivirals in patients with complex treatment histories. METHODS:All HCV mono-infected patients who received treatment at our institution were queried. Analysis was restricted to patients who previously failed t...
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-09-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i21.3217
更新日期:2005-06-07 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v16.i45.5773
更新日期:2010-12-07 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2008-04-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.3748/wjg.v11.i5.721
更新日期:2005-02-07 00:00:00
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pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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更新日期:2007-01-14 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v19.i11.1811
更新日期:2013-03-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
doi:10.3748/wjg.v21.i19.5762
更新日期:2015-05-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v26.i15.1733
更新日期:2020-04-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 信件
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更新日期:2013-01-21 00:00:00
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pub_type: 杂志文章
doi:10.3748/wjg.v22.i39.8844
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pub_type: 杂志文章,评审
doi:10.3748/wjg.v12.i17.2730
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2007-07-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i6.950
更新日期:2007-02-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i34.5266
更新日期:2005-09-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v16.i40.5042
更新日期:2010-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2014-03-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i23.4293
更新日期:2017-06-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2014-06-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v24.i32.3650
更新日期:2018-08-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v25.i33.4885
更新日期:2019-09-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2018-01-14 00:00:00