Purification and secondary structure determination of simian immunodeficiency virus p27.

Abstract:

:We have developed a novel method for the expression and purification of p27, the major core protein of simian immunodeficiency virus. Circular dichroism measurements of purified p27 were used to determine the relative amounts of alpha-helix, beta-sheet and unordered secondary structural elements. These empirically determined values appear to be inconsistent with previously published theoretical models based on homology comparisons.

journal_name

J Mol Biol

authors

Burns NR,Craig S,Lee SR,Richardsons MH,Stenner N,Adams SE,Kingsman SM,Kingsman AJ

doi

10.1016/s0022-2836(05)80308-0

subject

Has Abstract

pub_date

1990-11-20 00:00:00

pages

207-11

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(05)80308-0

journal_volume

216

pub_type

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