Abstract：
INTRODUCTION:This study was done to evaluate the mechanism involved in stem cell factor (SCF) expression and production by lipopolysaccharide (LPS)-stimulated odontoblast (OD)-like cells and to investigate the signal transduction pathway activated in the process. METHODS:ODs-like cells (MDPC-23) were stimulated with different LPS concentrations for 1, 6, and 24 hours. SCF expression in OD-like cells was analyzed by reverse-transcriptase polymerase chain reaction, and SCF production was assessed by enzyme-linked immunosorbent assay. In another set of experiments, OD-like cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD 98059 [PD]), p38 inhibitor (SB 202190 [SB]), or PI3K inhibitor (wortmannin [Wort]) for 30 minutes followed by stimulation with LPS (0.1 μg/mL) for 1 hour. RESULTS:OD-like cells stimulated with LPS (0.1 μg/mL) for 1 hour expressed SCF, but SCF production decreased with increasing LPS concentrations (1, 10, and 100 μg/mL). DEX and MK were able to inhibit SCF messenger RNA (mRNA) expression. PD, SB, and Wort inhibited the SCF mRNA expression. CONCLUSIONS:LPS-induced SCF mRNA expression and production in OD-like cells occur via leukotriene production or cytokine and/or chemokine formation, activating the p42/44, p38, and PI3K pathways. Data suggest that SCF released by OD-like cells may act as immune response modulators.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Oliveira SH,Santos VAdoi
10.1016/j.joen.2011.10.030subject
Has Abstractpub_date
2012-05-01 00:00:00pages
623-7issue
5eissn
0099-2399issn
1878-3554pii
S0099-2399(12)00100-8journal_volume
38pub_type
杂志文章abstract：INTRODUCTION:An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.06.019
更新日期：2010-09-01 00:00:00
abstract：:This study investigated the possibility of saliva recontamination occurring between the root canal wall and sealer through dentinal tubules exposed after the cementum was removed at the cervical level by root planing and treatment with citric acid. Thirty-four extracted human maxillary anterior teeth were randomly pla...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(96)80024-0
更新日期：1996-11-01 00:00:00
abstract：INTRODUCTION:The improvement of regenerative endodontic procedures requires an understanding of the key clinical questions combined with a fundamental biological knowledge of how the dental tissues behave during health, disease, and repair. Therefore, partnerships between clinicians and basic scientists are essential t...
journal_title：Journal of endodontics
pub_type： 杂志文章,评审
doi：10.1016/j.joen.2020.06.019
更新日期：2020-09-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to compare the ability of MTA (Dentsply Maillefer, Tulsa, OK) and EndoSequence Root Repair Material (ESRRM; Brasseler USA, Savannah, GA) to set in the presence of human blood and minimal essential media. METHODS:A model was created using polymethyl methacrylate blocks each pr...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.041
更新日期：2013-08-01 00:00:00
abstract：:The sealing ability of copal varnish has been investigated when used as an adjunct in restorative procedures, retrofilling procedures, and dontic obturation procedures. Barrier, a polyamide-type polymer cavity varnish, has been compared with copal varnishes in restorative dentistry. This investigation compared the api...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81138-6
更新日期：1992-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. METHODS:After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.10.002
更新日期：2012-02-01 00:00:00
abstract：INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.10.001
更新日期：2017-01-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effect of chitosan-hydroxyapatite precursor (C-HA) nanocomplex conditioning on the chemical modifications at the tricalcium silicate sealer-dentin interface using time-of-flight secondary ion mass spectrometry. METHODS:Dentin slabs from human premolar root den...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.08.011
更新日期：2019-12-01 00:00:00
abstract：:This study was carried out to investigate the effect of changes in tooth structure due to masticatory cycles on amalgam retrofillings performed to different cavity depths. Forty upper maxillary first molars were tested. In the obturated palatal root apical amalgam was inserted to different depths (1.5 and 3 mm). Eight...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(97)80169-0
更新日期：1997-10-01 00:00:00
abstract：:Advanced glycation end products (AGEs) have been found to play a role in inflammation and vascular complications. The receptor for AGE (RAGE) is expressed on many cells and is up-regulated during inflammation. To date, RAGE expression or its correlation with nuclear factor kappa beta (NF-kappabeta) expression has not ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.02.013
更新日期：2008-06-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the effect of various degrees of cyclic fatigue on torsional failure and torsional preloading on the cyclic fatigue life of heat-treated K3XF nickel-titanium (NiTi) instruments (SybronEndo, Orange, CA). METHODS:The mean number of cycles until failure (Nf) of K3XF ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.10.008
更新日期：2015-04-01 00:00:00
abstract：INTRODUCTION:Cross-sectional studies describe the health status of a population and measure the prevalence of disease or treatment. Neither the prevalence of periapical radiolucency, a surrogate for disease, nor the prevalence of root canal treatment have been subjected to a systematic review, which is the highest leve...
journal_title：Journal of endodontics
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.joen.2012.05.023
更新日期：2012-09-01 00:00:00
abstract：INTRODUCTION:Clinicians commonly mix mineral trioxide aggregate (MTA) to a desired consistency rather than use the recommended amounts of powder and water. The purpose of this in vitro study was to evaluate how varying the water-to-powder (WP) ratio affects the setting expansion of white MTA (WMTA) and gray MTA (GMTA)....
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2010.03.010
更新日期：2010-08-01 00:00:00
abstract：:The cleaning capacity of manual and rotary instrumentation techniques in mesial-distal flattened canals was studied by morphometric analysis. Twenty human mandibular incisors were divided into two groups of 10 teeth each: group 1, crown-down technique with rotary instrumentation using ProFile .04; group 2, crown-down ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200205000-00004
更新日期：2002-05-01 00:00:00
abstract：:The effectiveness of endodontic antimicrobial treatment could be determined using sensitive molecular methods. The purpose of this study was to determine if antibiotics or endodontic reagents interfere with the ability of PCR to detect Enterococcus faecalis in vitro. Amoxicillin (25 mg/ml), clindamycin (15 mg/ml), tet...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/01.don.0000152899.54247.4e
更新日期：2005-07-01 00:00:00
abstract：:Amalgam, IRM, and a mineral trioxide aggregate were tested for repair of experimentally created root perforations. Fifty sound, extracted mandibular and maxillary molars were used in this study. A perforation was created on the mesial root surface at about a 45-degree angle to the long axis of each tooth. The tooth wa...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81282-3
更新日期：1993-11-01 00:00:00
abstract：:A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81411-1
更新日期：1992-04-01 00:00:00
abstract：INTRODUCTION:Various factors may influence intracanal calcification in teeth treated with regenerative endodontic procedures. Bioactive materials, including ProRoot MTA (MTA; Dentsply Tulsa Dental Specialties, Memphis, TN) and Biodentine (BD; Septodont, Saint-Maur-des-Fossés, France), have been widely used as a coronal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.03.011
更新日期：2019-06-01 00:00:00
abstract：:Forty freshly extracted human teeth were biomechanically prepared for endodontic obturation using a radioisotopically labeled EDTA-urea peroxide-Carbowax compound. The amount of radiolabeled mixture remaining in the canals after through cleansing was about 3.8% of that originally applied. The amount of residue did not...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(75)80137-3
更新日期：1975-09-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) is a potent mitogenic and angiogenic factor involved in the development of many tissues including the teeth. We previously reported that dental pulp cells express the HGF receptor c-met and that HGF can enhance cell proliferation and differentiation. The purpose of this study was to dete...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.031
更新日期：2009-03-01 00:00:00
abstract：INTRODUCTION:Communication between pulp and periodontal tissue has been well established. However, it is unknown when periodontal disease begins to affect the clinical response of pulp tissue. The aim of this study was to assess the influence of periodontal severity on pulp sensibility by means of electric and thermal ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.07.006
更新日期：2017-11-01 00:00:00
abstract：INTRODUCTION:We evaluated the effect of various antimicrobials used in endodontic regeneration on a 3-week-old Enterococcus faecalis biofilm. METHODS:E. faecalis biofilm was grown on standardized dentin samples for 3 weeks. Infected dentin samples were randomized into 8 experimental groups (n = 8) and treated with cal...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2015.09.023
更新日期：2016-02-01 00:00:00
abstract：:Three randomly assigned groups of single-canaled extracted teeth obturated with gutta-percha were retreated using controlled application of one of three organic solvents: chloroform, xylene, or halothane. Two additional groups of teeth served as positive and negative controls. Residual volume of solvent expressed thro...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(98)80098-8
更新日期：1998-04-01 00:00:00
abstract：INTRODUCTION:The study was designed as a randomized double-blind trial to evaluate the anesthetic efficacy of 4% articaine with 1:100,000 epinephrine in inferior alveolar nerve block (IANB) and infiltration anesthetic techniques to anesthetize mandibular molars with irreversible pulpitis. METHODS:The study was compose...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2011.09.009
更新日期：2011-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this in-depth investigation was to identify, clarify, and substantiate clinical terminology relative to apical/periapical/periradicular diagnostic states, which is used routinely in the provision of endodontic care. Furthermore, the information gleaned from this investigation was used to lin...
journal_title：Journal of endodontics
pub_type： 共识发展会议,杂志文章
doi：10.1016/j.joen.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：INTRODUCTION:The aim of this study was to investigate the release of growth factors into root canal space after the irrigation procedure of regenerative endodontic procedure. METHODS:Sixty standardized root segments were prepared from extracted single-root teeth. Nail varnish was applied to all surfaces except the roo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.04.029
更新日期：2016-12-01 00:00:00
abstract：:This randomized, double-blind trial tested the null hypothesis that speed of deposition has no influence on the injection discomfort, efficacy, distribution, and duration of pulp anesthesia after incisive/mental nerve block in adult volunteers. Thirty-eight subjects received incisive/mental nerve blocks of 2.0 mL lido...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.07.016
更新日期：2007-10-01 00:00:00
abstract：INTRODUCTION:Complement activation is considered a major mechanism in innate immunity. Although it is mainly involved in initiating inflammation, recent data reported its involvement in other processes such as tissue regeneration. In the dental pulp, complement C5a fragment has been shown to be involved in the recruitm...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2016.06.011
更新日期：2016-09-01 00:00:00
abstract：:Ultrasonic instruments are a valuable asset for removing intraradicular posts from root canals before nonsurgical endodontic therapy. The purpose of this study was to evaluate the relative efficacy of post removal by ultrasonic and sonic devices. Fifty extracted teeth were instrumented and obturated. The canals were p...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80044-0
更新日期：1994-10-01 00:00:00
abstract：:This study assessed in vitro the diagnostic ability of cone beam computed tomography (CBCT) scans with different voxel resolutions in the detection of simulated external root resorption (ERR). For that purpose, 59 teeth were viewed through iCAT tomography (Imaging Sciences International, Inc, Hatfield, PA) following t...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2008.11.005
更新日期：2009-02-01 00:00:00
