Abstract:
PURPOSE:We showed previously that single nucleotide polymorphism (SNP) rs662702 in PAX6 may be located in a microRNA-328 binding site that causes susceptibility to high myopia. Our study was done to elucidate the role of PAX6 and its relationship with microRNA-328 in myopia. METHODS:A luciferase assay was used to confirm microRNA-328 binding to the PAX6 locus. Clones containing each allele of rs662702 were created and tested for their binding affinity to microRNA-328. Because a low level of PAX6 is a risk factor for myopia, we tested whether knockdown of PAX6 affects retinal pigment epithelial (RPE) cells and scleral cells, as well as expression of myopia-related genes. We also tested for the effect of retinoic acid (RA) on microRNA-328 expression, since RA-responsive elements are predicted to lie in the microRNA-328 promoter. RESULTS:MicroRNA-328 was shown to bind to the wild-type, but not mutant 3' untranslated region (UTR) of PAX6. The risk C allele of rs644242 had strong response to microRNA-328 but the protective T allele did not respond to microRNA-328. Down-regulation of PAX6 in RPE increased RPE proliferation, but reduced scleral cell proliferation. In addition, transforming growth factor (TGF)-β3 in the RPE and matrix malleoproteinase-2 (MMP2) in the sclera were increased, while collagen I and integrin β1 in the sclera were decreased. RA dose-dependently increased microRNA-328 expression and, in turn, suppressed PAX6 expression. CONCLUSIONS:We elaborated the relationship among myopia development, SNP rs662702, microRNA-328 and RA. The data imply that reduction of miR-328 and/or RA can be potential strategies for myopia prevention or treatment.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Chen KC,Hsi E,Hu CY,Chou WW,Liang CL,Juo SHdoi
10.1167/iovs.11-9272subject
Has Abstractpub_date
2012-05-31 00:00:00pages
2732-9issue
6eissn
0146-0404issn
1552-5783pii
iovs.11-9272journal_volume
53pub_type
杂志文章abstract:PURPOSE:This study was designed to examine whether retinal function can be rescued by allogeneic normal retinal pigment epithelial (RPE) grafts in Royal College of Surgeons (RCS) with retinal degeneration and, if so, whether this rescued function can be measured and followed by recording the corneal electroretinogram (...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Steady-state visual evoked potentials were elicited by the appearance/disappearance of sinusoidal gratings of varying spatial frequency for both eyes of amblyopic subjects. The amblyopic eyes showed reduced responses and a phase shift relative to the nonamblyopic eyes across a wide range of spatial frequencies. Both t...
journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1978-06-01 00:00:00
abstract:PURPOSE:To determine the mechanism of reattachment of harvested human retinal pigment epithelium (RPE) to RPE-derived extracellular matrix and Bruch's membrane. METHODS:Confluent first-to third-passage human RPE were harvested from tissue culture and plated onto RPE-derived extracellular matrix or human Bruch's membra...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract:PURPOSE:We evaluated the roles of tumor necrosis factor (TNF)-α in alkali-induced corneal neovascularization (CNV). METHODS:CNV was induced by alkali injury and compared in wild-type (WT) BALB/c mice, and TNF receptor 1-deficient (TNF-Rp55 KO) counterparts, or in mice treated with TNF-α antagonist and recombinant TNF-...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2012-06-14 00:00:00
abstract::Exposure of adult male and female albino rats to high-intensity illumination for 17.5 hr results in a marked, transient exophthalmos, which persists for approximately 48 hr after the light exposure. Upon examination of the Harderian glands in these rats, a significant glandular weight increase was observed up to 2 day...
journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1979-12-01 00:00:00
abstract:Purpose:To compare the phenotype of blue cone monochromacy (BCM) caused by large deletion mutations with those having the C203R missense mutation. Methods:BCM patients with large deletion mutations (n = 21; age range, 5-60 years), and with the C203R missense mutation (n = 13; age range, 5-70 years), were studied with ...
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
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abstract:PURPOSE:To investigate whether apoptosis plays a notable role in degeneration of corneal endothelial cells in patients with Fuchs' dystrophy. METHODS:Forty-seven corneal buttons from 41 patients with Fuchs' dystrophy were studied. Nucleus labeling, transmission electron microscopy (TEM), and TdT-dUTP terminal nick-end...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::PURPOSE. The purpose of the study was to investigate the effects of hyperglycemia on EGFR (epidermal growth factor receptor)-mediated wound response and signal transduction in the corneal epithelium of rats with type I diabetes mellitus (DM). METHODS. Corneal epithelia were removed from streptozotocin (STZ)- and weigh...
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更新日期:2004-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2017-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract:PURPOSE:The objective of this study was to determine whether alteration in mucins could be detected in patients with dry eye symptoms by using the monoclonal antibody H185, which recognizes carbohydrate epitopes on mucin molecules. METHODS:Immunohistochemistry and immunoelectron microscopy was used to examine binding ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract:PURPOSE:To investigate whether myopia is present in a breed of domestic dog, the Labrador retriever, and how the ocular components are related to refractive error in this breed. METHODS:Cycloplegic refractive error was measured in 75 Labrador retrievers by retinoscopy. Corneal and crystalline lens radii of curvature w...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:PURPOSE:To compare three microkeratome-assisted techniques for the preparation of ultrathin (UT) grafts for Descemet stripping automated endothelial keratoplasty. METHODS:After dissection with a 300-μm microkeratome head in 40 donor tissues, a second cut was performed with a 130-μm head either after manual stromal hyd...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2012-01-31 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:2001-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1998-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2016-12-01 00:00:00
abstract::Application of devices that degrade the retinal image has been reported to produce enlargement of the ocular globe in young domestic chicks. Two such device types (domes and arches) were applied to 3-day-old chicks. The domes affected the entire visual field whereas the arches affected only the lateral field. A third ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract:PURPOSE:To evaluate the absolute blood flow rate in a single choroidal vessel using Doppler optical coherence tomography (OCT). METHODS:Three choroidal vessels were selected in the right eye of three normal subjects, and were measured with Doppler OCT at a 1020-nm probe wavelength. The pulsatile change of the blood fl...
journal_title:Investigative ophthalmology & visual science
pub_type: 临床试验,杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
abstract::Cone electroretinograms were elicited with a full-field red flash after-45 min of dark adaptation from 16 patients with retinitis pigmentosa and from 17 normal subjects. The average latency and implicit time of the cone a-waves recorded from the patients were each significantly delayed (p less than 0.005) compared wit...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract:Purpose:To evaluate the corneal endothelial characteristics across the primary angle closure (PAC) disease spectrum amongst patients diagnosed as PAC suspects (PACS), PAC, PAC glaucoma (PACG), and previous acute PAC (APAC). Methods:We analyzed a total of 529 subjects (51 PACS, 170 PAC, 234 PACG, and 74 with previous A...
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更新日期:2018-09-04 00:00:00
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更新日期:2013-04-17 00:00:00
abstract:PURPOSE:Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material int...
journal_title:Investigative ophthalmology & visual science
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更新日期:2011-07-23 00:00:00
abstract::An analysis of size, shape, vacuole and cell counts of Schlemm's canal was undertaken in 20 human eyes that had undergone conventional immersion fixation. A reduction in vacuole count with age was shown to be associated with a decreased canal cell count. The decrease in cell count with age was closely linked to an age...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00