Right-first-time isocratic preparative liquid chromatography-mass spectrometry purification.

Abstract:

:Preparative RP-LCMS is widely used to purify compounds from crude reaction mixtures in drug discovery today. Method development for preparative Liquid Chromatography-Mass Spectrometry (LCMS) consists of finding the right balance between speed and quality, often leading to the use of fast generic gradients (5-95% organic mobile phase) to purify compounds of various structures and polarity, which can be sometimes detrimental to the purity of the isolated compound. Isocratic elution is the best method to obtain high purity, but it usually requires a long trial and error method development to find the right eluent composition. Therefore it is mostly used to purify large quantities of product using for instance stack injections, but not for routine purification of structurally diverse compounds. Herein, we describe an empirical method that allows "right-first-time" purifications with isocratic elution on preparative RP-LCMS, permitting isolation of compounds in high purity and yield.

journal_name

ACS Comb Sci

authors

Koza P,Gonnot V,Pelleter J

doi

10.1021/co200197b

subject

Has Abstract

pub_date

2012-04-09 00:00:00

pages

273-9

issue

4

eissn

2156-8952

issn

2156-8944

journal_volume

14

pub_type

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