Molecular cloning, characterization and expression analysis of B cell translocation gene 1 in grass carp Ctenopharyngodon idella.

Abstract:

:An expressed sequence tag (EST) of B cell translocation gene (BTG) 1 (gcbtg1) was obtained from a grass carp Ctenopharyngodon idellus intestinal complementary (c)DNA library and the full-length cDNA sequence was acquired by rapid amplification of cDNA ends (RACE) technology. The predicted Gcbtg1 protein contains the box A and box B motifs which characterized the BTG and transducer of ERBB2 (TOB) family. Multiple alignment analysis reveals that Gcbtg1 shares an overall identity of 65-94% with Gcbtg1s of other vertebrates. Real-time quantitative PCR analysis reveals that the highest expression level of gcbtg1 was detected in liver and the lowest in muscle. Western blotting analysis indicates that the immunological cross-reactivity occurs between C. idella and human Homo sapiens BTG1 protein. A 1008 bp 5'-flanking region sequence was cloned and analysed.

journal_name

J Fish Biol

journal_title

Journal of fish biology

authors

Fu YJ,Huang FG,Yuan T,Gu JR,Luo GQ,Xu H

doi

10.1111/j.1095-8649.2011.03200.x

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

669-78

issue

3

eissn

0022-1112

issn

1095-8649

journal_volume

80

pub_type

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