Oocyte slow freezing using a 0.2-0.3 M sucrose concentration protocol: is it really the time to trash the cryopreservation machine?

Abstract:

OBJECTIVE:To update results on outcomes with frozen/thawed oocytes using a differential sucrose concentration during dehydration (0.2 M) and rehydration (0.3 M), combined with a one-step propanediol exposure. DESIGN:Retrospective cohort study. SETTING:Private IVF centers. PATIENT(S):Infertile couples undergoing IVF treatment. INTERVENTION(S):Oocyte thawing cycles between May 2004 and December 2010. MAIN OUTCOME MEASURE(S):Survival, fertilization, and cleavage rates were reported to evaluate biological outcomes. Clinical pregnancy and implantation rates were analyzed as markers of efficiency. RESULT(S):Three hundred forty-two patients and 443 cycles were monitored; the survival was 71.8%, fertilization 77.9%, and of the embryos obtained 83.8% were classified as grade 1 and 2. Three hundred ninety-four transfers were performed, resulting in 90 pregnancies. The pregnancy rate per transfer was 22.8% and per patient was 26.3%, with 122 gestational sacs. The implantation rate per embryo was 13.5%. Patients were divided into three groups according to their age: ≤ 34 years (group A), 35-38 years (group B), and ≥ 39 years (group C). Biological outcomes were comparable in all three groups, whereas the pregnancy rate per transfer was higher in the first group (27.7% vs. 21.4% and 17.6%). The implantation rates per injected egg were 11.8%, 8.0%, and 7.5% for the three groups, respectively. CONCLUSION(S):The biological and clinical data obtained on 443 cycles are consistent with our previous results showing that slow freezing of oocytes can be a valid tool in IVF practice when performed with a suitable protocol.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Bianchi V,Lappi M,Bonu MA,Borini A

doi

10.1016/j.fertnstert.2012.01.127

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

1101-7

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(12)00186-0

journal_volume

97

pub_type

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