Abstract:
INTRODUCTION:Matrix extracellular phosphoglycoprotein (MEPE), a new member of the small integrin binding ligand N-glycosylated (SIBLING) family, is believed to play multifunctional roles in regulation of cell signaling, mineral homeostasis, and mineralization. METHODS:To study how MEPE affects the downstream genes involved in regulation of the proliferation and osteogenesis differentiation of dental pulp cells (DPCs), we explored the proliferation and osteogenesis differentiation capability of DPCs stimulated with recombinant MEPE or transfected with adenoviral-mediated human MEPE gene and used a systematic approach by osteogenesis real-time polymerase chain reaction arrays to profile osteogenesis-related gene expression after MEPE gene transfection. RESULTS:Our results indicated higher proliferation capability in a time- and dose-dependent pattern by cholecystokinin octapeptide assay, and gene/protein expression of osteogenic markers bone sialoprotein, dentin sialophosphoprotein, osteocalcin, and collagen I were up-regulated dependent on time points showed by real-time polymerase chain reaction and Western blot. Moreover, a total of 3 genes, including enamelin, transforming growth factor-β2, and integrin α2, were significantly up-regulated. CONCLUSIONS:These results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Wei X,Liu L,Zhou X,Zhang F,Ling Jdoi
10.1016/j.joen.2011.10.015subject
Has Abstractpub_date
2012-03-01 00:00:00pages
330-8issue
3eissn
0099-2399issn
1878-3554pii
S0099-2399(11)01204-0journal_volume
38pub_type
杂志文章abstract::The role of T-cells in the development of periapical lesions was investigated immunohistochemically using 16 normal (BALB/c) mice and 16 nude (BALB/c nu/nu) mice (congenitally T-cell-deficient mice). The pulp chambers of maxillar first molars of all mice were opened, and the infiltrated immunocytes (anti-Thy1.2, -Lyt-...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80565-0
更新日期:1995-04-01 00:00:00
abstract:INTRODUCTION:The authors conducted a prospective, randomized, single-blind study to determine the degree of pulpal anesthesia obtained with a primary infiltration of 1 cartridge of articaine in the incisive/mental nerve region of the mandibular second premolar and to determine the anesthetic efficacy of a repeat artica...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.11.039
更新日期:2013-03-01 00:00:00
abstract::This report describes the case of a patient with mesenchymal chondrosarcoma in the region of tooth #18 that mimicked apical periodontitis. Forty-five days after endodontic treatment, gingival swelling and intense pain were observed. The endodontist suspected a furcation lesion, and the treatment option that the patien...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.016
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:The dental pulp is highly vascularized and innervated tissue that is uniquely designed, being highly biologically active, while being enclosed within the calcified structure of the tooth. It is well-established that the dental pulp vasculature is a key requirement for the functional performance of the toot...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.06.033
更新日期:2020-09-01 00:00:00
abstract::EDTA-T and 10% citric acid used as root canal irrigants lead to more visible dentinal tubules than 5% sodium hypochlorite associated with 3% hydrogen peroxide. However, these cleansing agents must be compatible with apical periodontal tissue. We analyzed the cytotoxicity of 10% citric acid and EDTA-T in cultured fibro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200112000-00007
更新日期:2001-12-01 00:00:00
abstract:INTRODUCTION:Squamous odontogenic tumor-like epithelial islands occurring in the walls of odontogenic cysts are histologically identical to the squamous odontogenic tumor (SOT). Microscopically, the squamous odontogenic tumor-like proliferations (SOTLPs) share certain histologic features with SOT, acanthomatous and des...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.02.010
更新日期:2011-05-01 00:00:00
abstract::The purpose of this survey was to investigate the frequency and characteristics of surgery among active endodontists in the United States. The survey also inquired about residency and postresidency training in endodontic surgery. A Web-based questionnaire regarding endodontic surgery was distributed via e-mail to 2,53...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.10.008
更新日期:2009-01-01 00:00:00
abstract::The present study evaluates the morphological changes in root canal walls and temperature changes at root surfaces as a result of intracanal irradiation by erbium,chromium:YSGG laser under various conditions in vitro. Sixty single-rooted human teeth were examined. Root canals were prepared, and laser irradiation was p...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004770-200101000-00003
更新日期:2001-01-01 00:00:00
abstract::The purposes of this study were to determine quantitatively the amount of debris and irrigant forced in an apical direction, the frequency of apical plug development, and the time required to prepare canals when a step-back technique using K-files was compared with the .04 Taper system. Sixty-nine extracted teeth with...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80206-9
更新日期:1998-01-01 00:00:00
abstract:INTRODUCTION:Dens invaginatus is a critical condition for endodontic treatment once it frequently presents a complex internal anatomy and might be associated with incomplete root and apical development. METHODS:Three cases of dens invaginatus with open apex in maxillary lateral incisors are presented. In the first cas...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2010.02.002
更新日期:2010-06-01 00:00:00
abstract::K-type files, Sureflex NiTi files, and FlexoFiles were evaluated morphometrically as they come from the manufacturer, and after being used to instrument root canals 1, 3, and 5 times in maxillary premolars. Evaluation was made using a stereomicroscope at x40 magnification and photomicrographs. The abnormalities found ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(98)80046-0
更新日期:1998-07-01 00:00:00
abstract:INTRODUCTION:The choice of single- versus multiple-visit root canal treatment for infected teeth is in dispute. The purpose of this systematic review was to compare the healing rate and post-obturation pain of single- versus multiple-visit root canal treatment for teeth with infected root canals. METHODS:An exhaustive...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2010.09.005
更新日期:2011-02-01 00:00:00
abstract:INTRODUCTION:Dentin regeneration could be an ideal treatment option to restore tissue function. This study was conducted to evaluate the ability of dental pulp stem cells (DPSCs) and dentin matrix protein 1 (DMP1) impregnated within a collagen scaffold to regenerate dentin. METHODS:Simulated perforations were created ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.05.019
更新日期:2011-08-01 00:00:00
abstract:INTRODUCTION:This study evaluated the success rate of and tooth substance removal required for computer-guided preparation of endodontic access cavities. METHODS:Thirty acrylic typodont teeth with root canals (10 each of tooth numbers 11, 14, and 17) were randomly allocated to a study or control group (15 teeth per gr...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.10.025
更新日期:2021-02-01 00:00:00
abstract:INTRODUCTION:This study aimed to compare the success rates of direct pulp capping (DPC) by using either ProRoot Mineral Trioxide Aggregate (MTA) or Biodentine in the cariously exposed permanent teeth of 6- to 18-year-old patients. Gray discoloration was also evaluated. METHODS:Fifty-nine cariously exposed permanent te...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2017.10.012
更新日期:2018-03-01 00:00:00
abstract::Inflammation of pulp tissue appears as a consequence of caries progression. Its main characteristic is inflamed infiltrate whose cells contain lymphocytes. CD44 is a widely expressed adhesion molecule present in several body cells such as leukocytes and parenchymatous cells, including endothelial cells, epithelial cel...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2007.05.022
更新日期:2007-10-01 00:00:00
abstract::The aim of this study was to investigate the extent of calcium ion (Ca(2+)) removal on root dentin after treatment with 1.25% NaOCl, 2.5% NaOCl, 5.25% NaOCl, 2% chlorhexidine (CHX), 2% iodine potassium iodide (IKI), and MTAD in 3 immersion time periods. Extracted human mandibular premolars were bisected longitudinally...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.11.016
更新日期:2009-02-01 00:00:00
abstract::In this retrospective study with limited cone-beam computed tomography (limited CBCT), we investigated the anatomic characteristics of the accessory mental foramina and accessory branches of the mandibular canal. The CBCT records of approximately 150 patients were evaluated, and 17 accessory mental foramina were found...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.08.033
更新日期:2008-12-01 00:00:00
abstract::Vascular casts of cat premolar pulps in various stages of growth were made by injection of low-viscosity resin into the pulpal blood vessels. Examination under a scanning electron microscope revealed that, in the young pulp, the main arterioles ascend in the center of the pulp and venules run along the side of the roo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(89)80028-7
更新日期:1989-10-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to assess the ex vivo torsional performance of a novel rotary system in small root canals after 2 different glide path preparations. METHODS:Each independent canal of 8 mesial roots of mandibular molars was randomly assigned to achieve a reproducible glide path with a new set...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.034
更新日期:2017-03-01 00:00:00
abstract::Ultrasonic root-end preparation techniques have recently been introduced and revolutionized the field of endodontic surgery. The purpose of this study was to compare quantitatively the quality of root-end preparation techniques prepared by a specially designed ultrasonic retrotip with those prepared in a traditional m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80152-0
更新日期:1998-10-01 00:00:00
abstract::In the present study, we investigated the effect of the addition of different grades of gum rosins and hydrogenated resins to Grossman cement on dimensional stability, solubility and disintegration. pH and conductivity, which may affect these properties, were also determined. The experiments were performed according t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80284-2
更新日期:1999-07-01 00:00:00
abstract::Patients with odontogenic cutaneous sinus tracts usually seek treatment from a physician instead of a dentist and the etiology is frequently misdiagnosed. This case report describes a patient who sought treatment from a dentist because of a history of a previous cutaneous swelling on the ipsilateral side of his cheek....
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(06)80959-3
更新日期:1992-06-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to investigate the number of roots and the morphology of the root canals of maxillary and mandibular premolars in a selected German population. METHODS:Randomly selected full-size cone-beam computed tomographic images were collected from 700 patients (45% men and 55% women, avera...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.03.044
更新日期:2017-09-01 00:00:00
abstract::Inflammatory myofibroblastic tumors (IMTs) are rare. IMTs of the head and neck occur in all age groups, from neonates to old age, with the highest incidence occurring in childhood and early adulthood. An IMT has been defined as a histologically distinctive lesion of uncertain behavior. This article describes an unusua...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.09.006
更新日期:2015-12-01 00:00:00
abstract:INTRODUCTION:Two groups of patients with orofacial pains that are clinically important to distinguish from each other are patients with odontogenic pain and temporomandibular disorder (TMD) pain. The aim of this study was to determine the sensitivity and specificity of 2 screening instruments in distinguishing between ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.001
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:The treatment of immature necrotic permanent teeth presents several clinical challenges in endodontics. Regenerative endodontic procedures (REPs) permit root development, increased canal wall thickness, and apical closure. This longitudinal cohort study aimed to evaluate the long-term clinical and radiogra...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.10.035
更新日期:2017-03-01 00:00:00
abstract:INTRODUCTION:Calcium hydroxide has been used as a traditional pulpotomy agent for a long time but has some disadvantages. iRoot BP Plus (Innovative Bioceramix Inc, Vancouver, Canada) is a newly developed, ready-to-use calcium silicate-based bioactive ceramic with excellent bioactivity and sealing ability. However, whet...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.12.010
更新日期:2020-03-01 00:00:00
abstract::Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200306000-00011
更新日期:2003-06-01 00:00:00
abstract::A new endodontic obturation and retrofilling material has been invented to satisfy Grossman's requirements for an ideal material. Methylene blue dye leakage studies were carried out to compare the new material with conventional products, for both obturation and retrofilling. The experimental material when used in root...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200207000-00008
更新日期:2002-07-01 00:00:00