Abstract：
:The NF-κB family of transcription factors is activated in response to many environmental and biological stresses, and plays a key role in innate immunity across a broad evolutionary expanse of animals. A simple NF-κB pathway is present in the sea anemone Nematostella vectensis, an important model organism in the phylum Cnidaria. Nematostella has previously been shown to have two naturally occurring NF-κB alleles (Nv-NF-κB-C and Nv-NF-κB-S) that encode proteins with different DNA-binding and transactivation abilities. We show here that polymorphic residues 67 (Cys vs. Ser) and 269 (Ala vs. Glu) play complementary roles in determining the DNA-binding activity of the NF-κB proteins encoded by these two alleles and that residue 67 is primarily responsible for the difference in their transactivation ability. Phylogenetic analysis indicates that Nv-NF-κB-S is the derived allele, consistent with its restricted geographic distribution. These results define polymorphic residues that are important for the DNA-binding and transactivating activities of two naturally occurring variants of Nv-NF-κB. The implications for the appearance of the two Nv-NF-κB alleles in natural populations of sea anemones are discussed.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Wolenski FS,Chandani S,Stefanik DJ,Jiang N,Chu E,Finnerty JR,Gilmore TDdoi
10.1007/s00239-011-9479-7subject
Has Abstractpub_date
2011-12-01 00:00:00pages
325-36issue
5-6eissn
0022-2844issn
1432-1432journal_volume
73pub_type
杂志文章abstract：:Two types of gene encoding small subunits (SSU) of ADP-glucose pyrophosphorylase, a starch-biosynthetic enzyme, have been found in cereals and other grasses. One of these genes encodes two SSU proteins. These are targeted to different subcellular compartments and expressed in different organs of the plant: the endospe...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-007-9013-0
更新日期：2007-09-01 00:00:00
abstract：:Explicit evaluation of the accuracy and power of maximum likelihood and Bayesian methods for detecting site-specific positive Darwinian selection presents a challenge because selective consequences of single amino acid changes are generally unknown. We exploited extensive molecular and functional characterization of a...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-008-9172-7
更新日期：2008-11-01 00:00:00
abstract：:Intron-encoded U17 RNA is a member of the H/ACA box class of small nucleolar RNAs (snoRNAs) involved in ribosomal RNA (rRNA) maturation. U17 snoRNA shows typical characteristics of guide RNAs, which specify sites of pseudouridylation on the precursor rRNA (pre-rRNA). However, in spite of the presence of H and ACA boxe...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-001-0065-2
更新日期：2002-02-01 00:00:00
abstract：:The possibility of surface catalysed condensation of unsubstituted amino acids on kaolinite in aqueous systems at elevated temperatures was investigated; no evidence of clay catalysed polycondensation has been found. The thermodynamic feasibility of the hypothetical lysine/dilysine condensation reaction in the tempera...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01733261
更新日期：1978-12-29 00:00:00
abstract：:Long amino acid repeats are often observed in eukaryotic proteins. In humans, several neurological disorders are caused by proteins containing abnormally long polyglutamines. However, no systematic analysis has attempted to investigate the relationship between reiterations of particular amino acids and protein functio...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006601
更新日期：1999-12-01 00:00:00
abstract：:Liverworts are well supported as the sister group to all other land plants (embryophytes) by molecular data. Observations strongly supporting this earliest dichotomy in embryophyte evolution are the strikingly different introns occurring in the mitochondrial DNAs of liverworts versus non-liverwort embryophytes (NLE), ...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-010-9348-9
更新日期：2010-05-01 00:00:00
abstract：:Calmodulin is a calcium-binding EF-hand protein that is an activator of many enzymes as well as ion pumps and channels. Due to its multiple targets and its central role in the cell, understanding the evolutionary history of calmodulin genes should provide insights into the origin of genetic complexity in eukaryotes. W...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002390010074
更新日期：2000-08-01 00:00:00
abstract：:Computer analyses of various genome sequences revealed the existence of certain periodical patterns of adenine-adenine dinucleotides (ApA). For each genome sequence of 13 eubacteria, 3 archaebacteria, 10 eukaryotes, 60 mitochondria, and 9 chloroplasts, we counted frequencies of ApA dinucleotides at each downstream pos...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006541
更新日期：1999-08-01 00:00:00
abstract：:Fox and Woese (1975a) have shown that a model of 5S RNA secondary structure similar to the one originally derived for Chlorella 5S RNA can be generalized with relatively minor variations to all sequenced 5S RNA molecules, i.e. that corresponding base paired regions can be formed at approximately the same positions. We...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01796136
更新日期：1977-09-20 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02337518
更新日期：1996-11-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01732868
更新日期：1979-07-18 00:00:00
abstract：:Three measures of sequence dissimilarity have been compared on a computer-generated model system in which substitutions in random sequences were made at randomly selected sites and the replacement character was chosen at random from the set of characters different from the original occupant of the site. The three meas...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02602925
更新日期：1989-12-01 00:00:00
abstract：:The metabolic cycle of Saccharomyces cerevisiae consists of alternating oxidative (respiration) and reductive (glycolysis) energy-yielding reactions. The intracellular concentrations of amino acid precursors generated by these reactions oscillate accordingly, attaining maximal concentration during the middle of their ...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-009-9218-5
更新日期：2009-05-01 00:00:00
abstract：:Multiple genes of the hoatzin encoding stomach lysozyme c and closely related members of this calcium-binding lysozyme c group were cloned from a genomic DNA library and sequenced. There are a minimum of five genes represented among these sequences that encode two distinct groups of protein sequences. One group of thr...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02338801
更新日期：1996-06-01 00:00:00
abstract：:LINE-1 (L1) elements constitute the major family of retrotransposons in mammalian genomes. Here we report the first investigation of L1 evolution in New World monkeys (NWM). Two regions of the second open-reading frame were analyzed by two methods in three NWM species, the squirrel monkey (Saimiri sciureus), the tamar...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-003-2539-x
更新日期：2004-01-01 00:00:00
abstract：:The frequencies of ATG triplets in the genomes of various species were systematically analyzed, and the frequency of ATG triplets was significantly low around start codons in both prokaryotic and eukaryotic genomes. In eukaryotes, however, the frequency decrease before the start codon is much more evident than that af...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006460
更新日期：1999-02-01 00:00:00
abstract：:We describe the complete mitochondrial genome of the house centipede Scutigera coleoptrata. Its gene order is unique among characterized arthropod mitochondrial genomes. Comparison to the gene order in the horseshoe crab mtDNA implies 10 or more translocations. By extending comparisons to 30 arthropod mitochondrial ge...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-003-2563-x
更新日期：2004-04-01 00:00:00
abstract：:The alcohol dehydrogenase (Adh) gene has been isolated from Drosophila simulans and D. mauritiana by screening lambda clone libraries of each with a previously cloned Adh gene from D. melanogaster. The isolated lambda clones were subcloned and partially sequenced to determine the relatedness of these species and to ex...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02101983
更新日期：1984-01-01 00:00:00
abstract：:A comparison of mitochondrial DNA sequences was undertaken for two genes among orangutans from Borneo and Sumatra. The distribution of haplotypes among 42 individuals for NADH dehydrogenease subunit 3 and 39 individuals for cytochrome B was used to infer population structure. The haplotypes among all Bornean orangutan...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002390010110
更新日期：2000-11-01 00:00:00
abstract：:Wild-type Escherichia coli utilizes glycerol aerobically through an inducible pathway mediated by an ATP-dependent kinase and a glycerol 3-phosphate dehydrogenase which is a flavoprotein. A mutant, strain ECL424, employing a novel pathway for glycerol utilization was isolated. The novel pathway is mediated by an NAD-l...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02102318
更新日期：1983-01-01 00:00:00
abstract：:The weight-averaged observed/expected dinucleotide frequencies for the sum total of the coding regions of five Toxoplasma genes were compared with the same parameters previously determined for the coding regions of 21 Plasmodium genes. In addition, codon usage in the five Toxoplasma genes was compared with that in the...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02101892
更新日期：1990-04-01 00:00:00
abstract：:There has been considerable discussion in recent years on the evolution of the tandemly repeated multigene families, since some organisms show a concerted model whereas others show a birth-and-death model. This controversial subject extends to several species of fish. In this study, three species of the Sparidae famil...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-013-9541-8
更新日期：2013-03-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-007-9062-4
更新日期：2008-01-01 00:00:00
abstract：:Short interspersed DNA elements (SINEs) amplify by retroposition either by (i) successive waves of amplification from one or a few evolving master genes or by (ii) the generation of new master genes that coexist with their progenitors. Individual, highly conserved, elements of the B1 SINE family were identified from t...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002390010087
更新日期：2000-09-01 00:00:00
abstract：:Sequences were obtained from five species of rodents that are orthologous to an H2a histone pseudogene from Mus musculus. The pseudogene is part of the cluster of replication-dependent histone genes found on Mus musculus chromosome 13. Comparative analysis of these five sequences together with the previously published...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006312
更新日期：1998-03-01 00:00:00
abstract：:Analysis of cDNAs from Macrovipera lebetina transmediterranea (Mlt) and Echis ocellatus (Eo) venom gland libraries encoding disintegrins argued strongly for a common ancestry of the messengers of short disintegrins and those for precursors of dimeric disintegrin chains. We now report the sequence analysis of disintegr...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-006-0161-4
更新日期：2007-02-01 00:00:00
abstract：:We have performed DNA-DNA hybridization experiments among several species of Drosophila using the evolutionarily conserved portion of the genome representing sequences coding for amino acids of proteins. This was done by using as tracer, radioactively labeled complementary DNA that was reverse transcribed from adult m...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF00182390
更新日期：1992-02-01 00:00:00
abstract：:Synonymous codon usage in yeast appears to be influenced by natural selection on gene expression, as well as regional variation in compositional bias. Because of the large number of potential targets of selection (i.e., most of the codons in the genome) and presumed small selection coefficients, codon usage is an exce...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-003-2459-9
更新日期：2003-07-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01751807
更新日期：1977-11-25 00:00:00
abstract：:The activated dimonophosphate of 3'-deoxyadenosine (cordycepin) undergoes oligomerization to produce a new family of pyrophosphate-linked oligomers in which the average repeating unit involves a nine-atom structural group. The presence of a poly(U) template increases the relative yields of higher oligomers, although t...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02101146
更新日期：1987-01-01 00:00:00
