Abstract:
OBJECTIVE:To examine total ezrin expression (ezrin and phospho-ezrin) through the normal endometrial cycle and to correlate ezrin activation and localization with cytologic changes. DESIGN:Experimental laboratory study. SETTING:University medical centers. PATIENT(S):Reproductive-age women. INTERVENTION(S):A total of 36 samples of normal early, mid-, and late proliferative- and secretory-phase endometrium were studied for immunoreactive total ezrin (ir-T-ezrin) and phospho-ezrin (ir-p-ezrin) expression by histology, immunohistochemistry, and Western blotting. MAIN OUTCOME MEASURE(S):Total ezrin and phospho-ezrin expressions through the normal endometrial cycle. RESULT(S):Throughout the cycle ir-T-ezrin is present in the epithelium. The intensity and localization of both ir-ezrin and ir-p-ezrin vary greatly throughout the cycle. The main findings include the following: lateral localization of ir-ezrin/ir-p-ezrin in association with membrane specializations; dense staining around secretory vacuoles (secretory phase); dense staining of the apical surfaces, including microvilli and pinopodes of epithelial cells, especially during the mid- to late secretory phases; and the presence of ezrin in the glandular secretions. Immunoreactive total ezrin and ir-p-ezrin were not expressed by stromal fibroblasts. CONCLUSION(S):Ezrin is a prominent protein in the cycling endometrium. The most striking findings were the gravitation of ir-ezrin/ir-p-ezrin to the periphery of secretory vacuoles, localization on apical surfaces of the luminal epithelium, dense ezrin staining in secretory-phase epithelial cell plumes, and the presence of ir-ezrin/ir-p-ezrin in secretory-phase luminal secretions. These findings may have functional implications, especially for implantation biology.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Tan O,Ornek T,Fadiel A,Carrick KS,Arici A,Doody K,Carr BR,Naftolin Fdoi
10.1016/j.fertnstert.2011.10.039subject
Has Abstractpub_date
2012-01-01 00:00:00pages
192-9.e2issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(11)02724-5journal_volume
97pub_type
杂志文章abstract::Sequential treatment with clomiphene citrate, human menopausal gonadotropin, and human chorionic gonadotropin was successfully applied to 149 women in the framework of an in vitro fertilization and embryo transfer program and led to the birth of normal children. An average of 3.5 follicles was promoted at each cycle. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48382-2
更新日期:1985-02-01 00:00:00
abstract:OBJECTIVE:To perform a highly detailed semen analysis in a man whose wife had a partial mole. DESIGN:Case report. SETTING:Gynecology departments of two university hospitals and a laboratory of histology/embryology. PATIENT(S):A 32-year-old man whose wife had a partial mole. INTERVENTION(S):Sperm characteristics wer...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.01.161
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVE:The response to subcutaneous (SC) gonadotropin replacement therapy, using human chorionic gonadotropin (hCG) and human menopausal gonadotropin (hMG) or hCG alone, was evaluated in male hypothalamic hypogonadism. DESIGN:Sixteen patients with hypothalamic hypogonadism were treated with gonadotropins for induct...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54493-8
更新日期:1991-08-01 00:00:00
abstract::Of 299 patients evaluated for infertility between July 1, 1974, and December 31, 1976, 21 (7.2%) became pregnant during the initial evaluation prior to any recognized therapy. A comparative analysis was made between these 21 couples and the 223 completely evaluated infertility couples. No significant difference in sex...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1978-07-01 00:00:00
abstract::In a rat model, sodium carboxymethylcellulose (SCMC) was compared with normal saline and 32% dextran 70 in the prevention of surgically induced intraperitoneal adhesions. The 32% dextran 70 was similar to normal saline in the initial prevention of adhesions, and both appeared less effective than SCMC. After lysis of t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47910-0
更新日期:1984-06-01 00:00:00
abstract::A human embryo consisting of two cells, each containing two pronuclei, was found in the IVF laboratory. The most likely explanation for this phenomenon is a parthenogenetic activation of an oocyte leading to immediate cleavage and followed by double fertilization. Human chimerism may originate in this way. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53780-7
更新日期:1990-09-01 00:00:00
abstract::The effect of hyperprolactinemia during the midfollicular through the late follicular phase of the menstrual cycle on follicular development and follicular fluid hormone concentrations was studied. Fourteen women underwent laparotomy in the late follicular phase for gynecologic reasons; 6 were treated with sulpiride d...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49876-6
更新日期:1986-12-01 00:00:00
abstract::The Hammen semen quality classification system is a fertility index with six qualities based on sperm count, assessment of sperm motility, number of immotile spermatozoa, number of abnormal sperm heads, and sperm viability. It was developed by Hammen during the 1940s. In the present study, the fertility of 1077 men cl...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45624-4
更新日期:1981-07-01 00:00:00
abstract::Under guidance of an external pulsed magnetic field the Cuproferrogel iron oxide-copper-styrene maleic anhydride-dimethyl sulphoxide delivered into the rat/rabbit oviduct resulted in oocytes with granulated cytoplasm, zona enlargement, membrane disintegration, and finally loss of viability in 72 hours. Also, the perce...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.06.032
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To determine the efficiency of oocyte retrieval (OR) timing based on the occurrence of spontaneous LH surge during natural cycle IVF (ncIVF) treatment. DESIGN:Retrospective cohort study. The cohort was divided into five subgroups according to the presumed stage of spontaneous LH surge on scheduling day (1A: ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.01.016
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVE:To improve the maturation rate of murine and human germinal vesicle (GV) oocytes using human tubal epithelial cells (hTECs). DESIGN:Murine and human GV oocytes were randomized to human tubal fluid (HTF) media alone or cocultured with mouse embryonic fibroblasts (MEFs) or primary hTECs or immortalized hTECS (...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.08.034
更新日期:2011-03-15 00:00:00
abstract:OBJECTIVE:To predict pregnancy outcome and multiple gestation using a common parameter by which hCG values are made comparable independently of the day of blood sampling. DESIGN:Retrospective study. SETTING:University-based IVF program. PATIENTS:One hundred twenty IVF pregnancies conceived between November, 1995 and...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03278-8
更新日期:2002-09-01 00:00:00
abstract:OBJECTIVE:To examine the usefulness of a trial cycle of hormone replacement therapy (HRT) and endometrial biopsy before the actual ET cycle in recipients of donated oocytes. DESIGN:Retrospective review. SETTING:Clinical practice at the South Texas Fertility Center, San Antonio, Texas. PATIENT(S):Thirty-six concurren...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00165-4
更新日期:1998-08-01 00:00:00
abstract:OBJECTIVE:To create, validate, and use a fertility awareness survey based on current U.S. DATA: DESIGN:Cross-sectional study. SETTING:Not applicable. PATIENT(S):Phase 1 included U.S. women ages 18-45; phase 2 included female medical students and obstetrics and gynecology trainees at two urban academic programs. IN...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2017.07.1158
更新日期:2017-10-01 00:00:00
abstract::The administration of five subcutaneous 250-microgram doses of lutienizing hormone (LH)-releasing hormone (LHRH) at 4-hour intervals, the first injection being given at 8 A.M. on 1 or 2 consecutive days between days 1 and 9 following the LH surge in normal women, shortened the luteal phase from 1 to 4 days in 16 of 17...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)43755-6
更新日期:1979-01-01 00:00:00
abstract:OBJECTIVE:To describe a woman with a nonmosaic (45,X) form of Turner's syndrome who gave birth to a girl with 45,X Turner syndrome. DESIGN:Patient report. SETTING:Outpatient clinic of a university hospital. PATIENT(S):A woman with typical phenotypic features of Turner syndrome and a 45,X karyotype and her daughter w...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.02.129
更新日期:2004-10-01 00:00:00
abstract::For determination of how exposure of the monkey follicle to the preovulatory luteinizing hormone (LH)/follicle-stimulating hormone (FSH) surge alters its responsiveness to FSH in terms of estrogen and progesterone secretory ability, monkey thecal tissue and granulosa cells were harvested prior to and during the midcyc...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48087-8
更新日期:1984-09-01 00:00:00
abstract::Testosterone and 19-nortestosterone derivatives were evaluated in a developmental scheme designed to identify competitive progesterone antagonists having abortifacient activity. Compounds that displayed significant binding to the rabbit uterine progesterone receptor were followed in biologic tests for progestational, ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44003-3
更新日期:1979-05-01 00:00:00
abstract:OBJECTIVE:To assess racial differences in attitudes and knowledge about sterilization. DESIGN:Cross-sectional survey. SETTING:Questionnaires were mailed to participants' home addresses. PATIENT(S):One hundred ninety-three women, aged 18-45, who had undergone tubal sterilization. INTERVENTION(S):None. MAIN OUTCOME ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.05.031
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To determine the influence of estrogens on male fertility. DESIGN:A 36-year-old eugonadal male was subjected to two different regimens of treatment with ethinyl estradiol (EE2). Sperm quality, immunoreactive luteinizing hormone (LH) and follicle-stimulating Hormone (FSH), testosterone (T), estrone (E1), estr...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55271-6
更新日期:1992-09-01 00:00:00
abstract:OBJECTIVE:To determine the effect of P on nitric oxide synthase (NOS) expression in human endometrial epithelial cells. DESIGN:Laboratory-based study. SETTING:University-based research institute. PATIENT(S):None. INTERVENTION(S):The effect of P on the expression of NOS protein isoforms was examined in an in vitro p...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.05.019
更新日期:2009-05-01 00:00:00
abstract::Ham's F-10 medium (Gibco, Grand Island, NY) and medium T6 with and without 15% fetal calf serum (FCS) were compared for their ability to support development of murine blastocysts with the capacity to implant and produce normal fetuses when transferred to pseudopregnant females. All media supported equal rates of blast...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59545-4
更新日期:1987-11-01 00:00:00
abstract:OBJECTIVE(S):To investigate the pattern of follicular development in transplanted fetal and neonatal mouse ovaries in the presence or absence of the recipient's own ovaries. DESIGN:Controlled experiment. SETTING:Academic research laboratory, Department of Physiology, Monash University, Clayton, Australia. INTERVENTI...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00635-x
更新日期:2000-08-01 00:00:00
abstract:OBJECTIVE:To study the chromosomal content of spermatozoa selected by intracytoplasmic morphologically selected sperm injection (IMSI) in cases of macrocephalic sperm head syndrome. DESIGN:Case report. SETTING:Obstetrics, gynecology, urology, and reproductive biology departments. PATIENT(S):Two infertile patients wi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.10.059
更新日期:2010-03-01 00:00:00
abstract::Of 5,341 infertile couples seen over an 8-year period, 76 (1.4%) had primary vaginismus, of whom 63 were treated with the use of a simplified sensate focus technique. There was complete symptomatic resolution of vaginismus in 60 women, and pregnancy was achieved in 33. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.071
更新日期:2010-11-01 00:00:00
abstract::Embryo transfer is arguably the most critical process in the sequential events that encompass an IVF cycle. Several variables play a role in the success of a transfer, including catheter type, atraumatic technique, and the use of ultrasound guidance. The inclusion of hyaluronan in the ET media also has a benefit for i...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2016.02.022
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVE:Racial disparity in assisted reproductive technology (ART) outcomes has been reported but remains controversial. Reasons for the disparity are unclear, and access to care has been suggested as a causative factor. In this study, we sought to examine minority utilization of ART in the Department of Defense (DoD...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.10.028
更新日期:2006-04-01 00:00:00
abstract:OBJECTIVE:To study the safety and efficacy of ovarian stimulation and oocyte cryopreservation as a method of fertility preservation in women with Turner syndrome (TS). DESIGN:Retrospective cohort study. SETTING:Reproductive medicine clinic. PATIENT(S):Seven women with TS who attended the clinic between 2011 and 2017...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.11.010
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:To investigate c-fos and matrix metalloproteinase-9 (MMP-9) expression in the endometrium from women with or without endometriosis throughout the menstrual cycle, and to explore the correlation of c-fos expression with MMP-9 expression and 17beta-E(2) levels in serum. DESIGN:Molecular studies in human tissue...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.07.1386
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVE:To evaluate the association between follicular and serum nitric oxide (NO) levels and embryo development and outcome in IVF. DESIGN:Prospective, case-control study. SETTING:University hospital, tertiary medical center. PATIENT(S):Eighteen patients with tubal or peritoneal factor infertility and 18 female p...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.02.097
更新日期:2004-07-01 00:00:00