Expression and activation of the membrane-cytoskeleton protein ezrin during the normal endometrial cycle.

Abstract:

OBJECTIVE:To examine total ezrin expression (ezrin and phospho-ezrin) through the normal endometrial cycle and to correlate ezrin activation and localization with cytologic changes. DESIGN:Experimental laboratory study. SETTING:University medical centers. PATIENT(S):Reproductive-age women. INTERVENTION(S):A total of 36 samples of normal early, mid-, and late proliferative- and secretory-phase endometrium were studied for immunoreactive total ezrin (ir-T-ezrin) and phospho-ezrin (ir-p-ezrin) expression by histology, immunohistochemistry, and Western blotting. MAIN OUTCOME MEASURE(S):Total ezrin and phospho-ezrin expressions through the normal endometrial cycle. RESULT(S):Throughout the cycle ir-T-ezrin is present in the epithelium. The intensity and localization of both ir-ezrin and ir-p-ezrin vary greatly throughout the cycle. The main findings include the following: lateral localization of ir-ezrin/ir-p-ezrin in association with membrane specializations; dense staining around secretory vacuoles (secretory phase); dense staining of the apical surfaces, including microvilli and pinopodes of epithelial cells, especially during the mid- to late secretory phases; and the presence of ezrin in the glandular secretions. Immunoreactive total ezrin and ir-p-ezrin were not expressed by stromal fibroblasts. CONCLUSION(S):Ezrin is a prominent protein in the cycling endometrium. The most striking findings were the gravitation of ir-ezrin/ir-p-ezrin to the periphery of secretory vacuoles, localization on apical surfaces of the luminal epithelium, dense ezrin staining in secretory-phase epithelial cell plumes, and the presence of ir-ezrin/ir-p-ezrin in secretory-phase luminal secretions. These findings may have functional implications, especially for implantation biology.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Tan O,Ornek T,Fadiel A,Carrick KS,Arici A,Doody K,Carr BR,Naftolin F

doi

10.1016/j.fertnstert.2011.10.039

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

192-9.e2

issue

1

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(11)02724-5

journal_volume

97

pub_type

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