Comparison of the cytogenotoxicity induced by five different dental alloys using four in vitro assays.

Abstract:

:The purpose of present investigation was to compare the cyto-genotoxicity induced by five dental alloys in vitro. The cyto-genotoxicity induced by five dental alloy extracts on human B lymphoblast cells was assessed with neutral red uptake (NRU) , CCK-8, comet and micronucleus (CBMN) assays in vitro, respectively. The results of in vitro comet and CBMN assays indicated that DNA damage (% tail DNA) and micronucleus frequencies (MNFs) in all exposure groups did not significantly increase, as compared with the control group. However, the results of NRU and CCK-8 assays demonstrated that there were to some extent differences in the cytotoxicity among 5 dental alloy extracts in vitro. The cytotoxicity may be relevant to the Ni and Be ions released in the alloy extract.

journal_name

Dent Mater J

journal_title

Dental materials journal

authors

Zhihong C,Yezhen L,Zhiyuan G,Zhongqiao Y,Xiaoxue L,Yifeng R,Weikun F,Jiliang H

doi

10.4012/dmj.2011-127

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

861-8

issue

6

eissn

0287-4547

issn

1881-1361

pii

JST.JSTAGE/dmj/2011-127

journal_volume

30

pub_type

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