Pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal polypeptide promote the genesis of calcium currents in differentiating mouse embryonic stem cells.

Abstract:

:Identification of novel molecules that can induce neuronal differentiation of embryonic stem (ES) cells is essential for deciphering the molecular mechanisms of early development and for exploring cell therapy approaches. Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) are known to be implicated early during ontogenesis in cell proliferation and neuronal differentiation. The aim of the present study was to determine the effects of VIP and PACAP on functional differentiation of ES cells. Quantitative-reverse transcription-polymerase chain reaction analysis showed an inversion of the expression pattern of PAC1 and VPAC1 receptors with time. ES cells expressed genes encoding extracellular signal-regulated kinase 1 and 2 and c-jun amino terminal kinase1. ES cells also expressed T-type α1I and α1G, L-type α1C and α1D, and N-type α1B calcium channel subunit mRNAs. Both peptides modified the shape of undifferentiated ES cells into bipolar cells expressing the neuronal marker neuron-specific enolase (NSE). Immunostaining indicated that PACAP intensified T-type α1I subunit immunoreactivity, whereas VIP increased L-types α1C and α1D, as well as N-type α1B subunit. Electrophysiological recording showed that VIP and PACAP enhanced transient calcium current. Moreover, VIP generated sustained calcium current. These findings demonstrate that PACAP and VIP induce morphological and functional differentiation of ES cells into a neuronal phenotype. Both peptides promote functional maturation of calcium channel subunits, suggesting that they can facilitate the genesis of cellular excitability.

journal_name

Neuroscience

journal_title

Neuroscience

authors

Chafai M,Basille M,Galas L,Rostene W,Gressens P,Vaudry H,Gonzalez BJ,Louiset E

doi

10.1016/j.neuroscience.2011.09.062

subject

Has Abstract

pub_date

2011-12-29 00:00:00

pages

103-15

eissn

0306-4522

issn

1873-7544

pii

S0306-4522(11)01148-1

journal_volume

199

pub_type

杂志文章
  • Kainate-glutamate interactions in rat cerebellar slices.

    abstract::The effects of L-glutamate on the potency of kainate for stimulating guanosine 3',5'-cyclic monophosphate (cyclic GMP) accumulation and for killing neurones in incubated slices of immature (8-day) and adult rat cerebellum were investigated. L-glutamate did not potentiate the cyclic GMP responses to kainate in either t...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/0306-4522(84)90218-5

    authors: Garthwaite J,Gilligan GJ

    更新日期:1984-01-01 00:00:00

  • Cocaine mechanisms: enhanced cocaine, fluoxetine and nisoxetine place preferences following monoamine transporter deletions.

    abstract::Cocaine blocks uptake by neuronal plasma membrane transporters for dopamine, serotonin and norepinephrine, producing subjective effects in humans that are both euphoric/rewarding and also fearful, jittery and aversive. Mice with gene knockouts of each of these transporters display cocaine reward, manifest by cocaine p...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(02)00379-2

    authors: Hall FS,Li XF,Sora I,Xu F,Caron M,Lesch KP,Murphy DL,Uhl GR

    更新日期:2002-01-01 00:00:00

  • Selective gastric projections of nitric oxide synthase-containing vagal brainstem neurons.

    abstract::Nitric oxide has been proposed to act as an intercellular messenger in central brainstem circuits controlling gastrointestinal motility. In particular, a subpopulation of preganglionic vagal neurons of the dorsal motor nucleus of the vagus have been shown to be reduced nicotinamide adenine dinucleotide phosphate(NADPH...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(98)00586-7

    authors: Zheng ZL,Rogers RC,Travagli RA

    更新日期:1999-05-01 00:00:00

  • The interaction of emotion and pain in the insula and secondary somatosensory cortex.

    abstract::Pain is processed in a large neural network that partially overlaps structures involved in emotion processing. Despite the fact that pain and emotion are known to share neural regions and interact in numerous clinical conditions, relatively little is known about the interaction of pain and emotion at the neural level....

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2017.02.047

    authors: Orenius TI,Raij TT,Nuortimo A,Näätänen P,Lipsanen J,Karlsson H

    更新日期:2017-05-04 00:00:00

  • Ethanol modulates mammalian circadian clock phase resetting through extrasynaptic GABA receptor activation.

    abstract::Ethanol modulates the actions of multiple neurotransmitter systems, including GABA. However, its enhancing effects on GABA signaling typically are seen only at high concentrations. In contrast, although GABA is a prominent neurotransmitter in the circadian clock of the suprachiasmatic nucleus (SCN), we see ethanol mod...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2009.08.020

    authors: McElroy B,Zakaria A,Glass JD,Prosser RA

    更新日期:2009-12-01 00:00:00

  • Glial cell line-derived neurotrophic factor is crucial for long-term maintenance of the nigrostriatal system.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) is a potent factor for the ventral mesencephalic dopamine neurons. However, studies on the Gdnf gene deleted (Gdnf(-/-)) mouse have been limited to fetal tissue since these mice die prematurely. To evaluate long-term effects of Gdnf gene deletion, this study involves ...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2010.10.010

    authors: Nevalainen N,Chermenina M,Rehnmark A,Berglöf E,Marschinke F,Strömberg I

    更新日期:2010-12-29 00:00:00

  • Glycogen synthase kinase-3 reduces acetylcholine level in striatum via disturbing cellular distribution of choline acetyltransferase in cholinergic interneurons in rats.

    abstract::Cholinergic interneurons, which provide the main source of acetylcholine (ACh) in the striatum, control the striatal local circuits and deeply involve in the pathogenesis of neurodegenerative diseases. Glycogen synthase kinase-3 (GSK-3) is a crucial kinase with diverse fundamental functions and accepted that deregulat...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2013.10.001

    authors: Zhao L,Chu CB,Li JF,Yang YT,Niu SQ,Qin W,Hao YG,Dong Q,Guan R,Hu WL,Wang Y

    更新日期:2013-01-01 00:00:00

  • Behavioral analysis of opiate-mediated inhibition in the early chick embryo.

    abstract::Morphine (opiate agonist) produced a dose-dependent decrease in the spontaneous motility of 5- and 9-day chick embryos. Naloxone (opiate antagonist) appeared to reverse competitively the inhibition of motility caused by morphine. The effects of morphine on spontaneous motility in 5-day embryos were also reversed stere...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/0306-4522(85)90012-0

    authors: Maderdrut JL,Reitzel JL,Okado N,Oppenheim RW

    更新日期:1985-10-01 00:00:00

  • A closer look at mechanisms underlying perceptual differences in Parkinson's freezers and non-freezers.

    abstract::Parkinson's disease patients who suffer from freezing of gait (PD-FOG) may have sensory and/or perceptual deficits, although they are difficult to disentangle. This study evaluated whether visuospatial perception or self-motion perception were more impaired in PD-FOG, and whether distance estimation errors might be re...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2014.05.022

    authors: Ehgoetz Martens KA,Ellard CG,Almeida QJ

    更新日期:2014-08-22 00:00:00

  • Alterations in the ryanodine receptor calcium release channel correlate with Alzheimer's disease neurofibrillary and beta-amyloid pathologies.

    abstract::Investigation of the integrity of the ryanodine receptor in Alzheimer's disease is important because it plays a critical role in the regulation of calcium release from the endoplasmic reticulum in brain, impairment of which is believed to contribute to the pathogenesis of Alzheimer's disease. The present study compare...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(99)00042-1

    authors: Kelliher M,Fastbom J,Cowburn RF,Bonkale W,Ohm TG,Ravid R,Sorrentino V,O'Neill C

    更新日期:1999-01-01 00:00:00

  • Time-course of hippocampal granule cell degeneration and changes in adult neurogenesis after adrenalectomy in rats.

    abstract::The hippocampus maintains the remarkable ability to generate new neurons throughout the lifespan. Progenitor cells in the subgranular zone give rise mainly to granule cells that migrate to the granule cell layer and become mature, functionally integrated neurons. Numerous factors are capable of regulating the prolifer...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2011.06.023

    authors: Spanswick SC,Epp JR,Sutherland RJ

    更新日期:2011-09-08 00:00:00

  • Comparative immunohistochemical localisation of GABA(B1a), GABA(B1b) and GABA(B2) subunits in rat brain, spinal cord and dorsal root ganglion.

    abstract::GABA(B) receptors are G-protein-coupled receptors mediating the slow onset and prolonged synaptic actions of GABA in the CNS. The recent cloning of two genes, GABA(B1) and GABA(B2), has revealed a novel requirement for GABA(B) receptor signalling. Studies have demonstrated that the two receptor subunits associate as a...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(01)00296-2

    authors: Charles KJ,Evans ML,Robbins MJ,Calver AR,Leslie RA,Pangalos MN

    更新日期:2001-01-01 00:00:00

  • Expression of angiotensin II and its receptors in the normal and hypoxic amoeboid microglial cells and murine BV-2 cells.

    abstract::Involvement of the local angiotensin receptor system in the central nervous system is well documented, yet its cellular localization and role in the glial cells have remained elusive. This study reports expression of angiotensin II and its receptors namely, angiotensin II receptor type 1 (AT(1)) and angiotensin II rec...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2008.11.046

    authors: Li JJ,Lu J,Kaur C,Sivakumar V,Wu CY,Ling EA

    更新日期:2009-02-18 00:00:00

  • Calcium-dependent inactivation of neuronal calcium channel currents is independent of calcineurin.

    abstract::Dephosphorylation by the Ca2+/calmodulin-dependent phosphatase calcineurin has been suggested as an important mechanism of Ca2+-dependent inactivation of voltage-gated Ca2+ channels. We have tested whether calcineurin plays a role in the inactivation process of two types of high-voltage-activated Ca2+ channels (L and ...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(99)00434-0

    authors: Zeilhofer HU,Blank NM,Neuhuber WL,Swandulla D

    更新日期:2000-01-01 00:00:00

  • Ultrastructural studies on peptides in the dorsal horn of the rat spinal cord--III. Effects of peripheral axotomy with special reference to galanin.

    abstract::In this study co-localization of galanin- with calcitonin gene-related peptide (CGRP)-like immunoreactivity was examined in dorsal root ganglion neurons 14 days after sciatic nerve cut using a laser scanning confocal microscope. CGRP- and galanin-like immunoreactivities were also analysed in the dorsal horn of the spi...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/0306-4522(94)00452-b

    authors: Zhang X,Bean AJ,Wiesenfeld-Hallin Z,Xu XJ,Hökfelt T

    更新日期:1995-02-01 00:00:00

  • Adolescent social defeat increases adult amphetamine conditioned place preference and alters D2 dopamine receptor expression.

    abstract::Components of the brain's dopaminergic system, such as dopamine receptors, undergo final maturation in adolescence. Exposure to social stress during human adolescence contributes to substance abuse behaviors. We utilized a rat model of adolescent social stress to investigate the neural mechanisms underlying this corre...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2011.09.008

    authors: Burke AR,Watt MJ,Forster GL

    更新日期:2011-12-01 00:00:00

  • The pH dependence of late sodium current in large sensory neurons.

    abstract::The effects of altering extracellular pH on late Na+ currents were investigated in large dorsal root ganglion neurons from rats (100-300 g), using patch-clamp techniques. The late current amplitude was steeply dependent upon pH over a range which included normal physiological values: raising the pH from 7.3 to 8.3 app...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(99)00058-5

    authors: Baker MD,Bostock H

    更新日期:1999-01-01 00:00:00

  • Expression of PAC 1, an epitope associated with two synapse-enriched glycoproteins and a neuronal cytoskeleton-associated polypeptide in developing forebrain neurons.

    abstract::The monoclonal antibody PAC 1 (postsynaptic density and cytoskeleton enriched) recognizes an epitope present on two postsynaptic density-enriched glycoproteins of 130,000 (postsynaptic density-enriched glycoprotein 130) and 117,000 mol. wt (postsynaptic density-enriched glycoprotein 117), and a cytoskeleton-enriched p...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/0306-4522(94)90159-7

    authors: Willmott T,Williamson TL,Mummery R,Hawkes RB,Can A,Gurd JW,Gordon-Weeks PR,Beesley PW

    更新日期:1994-01-01 00:00:00

  • The amnesic effect of intra-central amygdala administration of a cannabinoid CB1 receptor agonist, WIN55,212-2, is mediated by a β-1 noradrenergic system in rat.

    abstract::In this study, we investigated effects of intra-central amygdala (intra-CeA) administrations of a cannabinoid agonist, WIN55,212-2 by itself and its interaction with β1-adrenoceptor agents on memory consolidation. We used a step-through inhibitory avoidance (IA) task to assess memory in male Wistar rats. The results s...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2012.04.008

    authors: Zarrindast MR,Ghiasvand M,Rezayof A,Ahmadi S

    更新日期:2012-06-14 00:00:00

  • Metabotropic glutamate receptor 5 modulates calcium oscillation and innate immune response induced by lipopolysaccharide in microglial cell.

    abstract::Microglia, the primary immune cells in the brain, have been implicated as the predominant cells governing inflammation-mediated neuronal damage. In response to immunological challenges such as lipopolysaccharide (LPS), microglia are activated and subsequently inflammatory process is initiated as evidenced by the relea...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2014.09.034

    authors: Liu F,Zhou R,Yan H,Yin H,Wu X,Tan Y,Li L

    更新日期:2014-12-05 00:00:00

  • Two receptors are involved in the central functions of kynurenic acid under an acute stress in neonatal chicks.

    abstract::Intracerebroventricular (i.c.v.) injection of kynurenic acid (KYNA) had sedative and hypnotic effects during stress in neonatal chicks. However, its mechanism has not been clarified. KYNA is an endogenous antagonist of the α7 nicotinic acetylcholine (α7nACh) receptor and N-methyl-d-aspartate (NMDA) receptor. Therefore...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2013.06.005

    authors: Yoshida J,Shigemura A,Ogino Y,Denbow DM,Furuse M

    更新日期:2013-09-17 00:00:00

  • Neurotensin-containing projections from the retrosplenial cortex to the anterior ventral thalamic nucleus in the rat.

    abstract::The neurotensin-containing projections from the retrosplenial cortex to the anterior ventral thalamus were demonstrated by electrolytic lesion studies and fluorescent retrograde tracing combined with immunocytochemistry. Three-to-five-day-old rats were used, because the immunoreactivity of neurotensin fibers in anteri...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/0306-4522(88)90102-9

    authors: Fukami K,Kiyama H,Shiotani Y,Tohyama M

    更新日期:1988-09-01 00:00:00

  • l-DOPA-induced dyskinesia is associated with a deficient numerical downregulation of striatal tyrosine hydroxylase mRNA-expressing neurons.

    abstract::l-3,4-Dihydroxyphenylalanine (l-DOPA) is the therapeutic gold standard in Parkinson's disease. However, most patients develop debilitating abnormal involuntary movements termed l-DOPA-induced dyskinesia (LID) as therapy-complicating side effects. The underlying mechanisms of LID pathogenesis are still not fully unders...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2016.06.017

    authors: Klietz M,Keber U,Carlsson T,Chiu WH,Höglinger GU,Weihe E,Schäfer MK,Depboylu C

    更新日期:2016-09-07 00:00:00

  • Peripherally administered ghrelin induces bimodal effects on the mesolimbic dopamine system depending on food-consumptive states.

    abstract::Ghrelin induces orexigenic behavior by activation of growth hormone secretagogue 1 receptors (GHSRs) in the ventral tegmental area (VTA) as well as hypothalamus, suggesting the involvement of mesolimbic dopamine system in the action of ghrelin. The present study aimed to identify neuronal mechanisms by which periphera...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2009.03.086

    authors: Kawahara Y,Kawahara H,Kaneko F,Yamada M,Nishi Y,Tanaka E,Nishi A

    更新日期:2009-07-07 00:00:00

  • Tract-based analysis of white matter degeneration in Alzheimer's disease.

    abstract::Although much prior work has focused on the known cortical pathology that defines Alzheimer's disease (AD) histologically, recent work has additionally demonstrated substantial damage to the cerebral white matter in this condition. While there is large evidence of diffuse damage to the white matter in AD, it is unclea...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2015.05.049

    authors: Lee SH,Coutu JP,Wilkens P,Yendiki A,Rosas HD,Salat DH,Alzheimer’s disease Neuroimaging Initiative (ADNI).

    更新日期:2015-08-20 00:00:00

  • Expression of X-chromosome linked inhibitor of apoptosis protein in mature Purkinje cells and in retinal bipolar cells in transgenic mice induces neurodegeneration.

    abstract::Transgenic mice with overexpression of the caspase-inhibitor, X-chromosome-linked inhibitor of apoptosis protein (XIAP) in Purkinje cell (PC) and in retinal bipolar cells (RBCs) were produced to study the regulation of cell death. Unexpectedly, an increased neurodegeneration was observed in the PCs in these L7-XIAP mi...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2008.08.005

    authors: Korhonen L,Hansson I,Maugras C,Wehrle R,Kairisalo M,Borgkvist A,Jokitalo E,Sotelo C,Fisone G,Dusart I,Lindholm D

    更新日期:2008-10-15 00:00:00

  • Induction of heat-shock protein-70 messenger RNA and protein following systemic kainate injection in the rat: evidence of protein axonal transport.

    abstract::Induction of heat-shock protein-70 was studied using in situ hybridization and immunohistochemistry in the adult rat at different times following intraperitoneal injection of kainate. Marked expression of heat-shock protein-70 messenger RNA was observed during the first 24 h, followed by residual signal at 48 h. Induc...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/0306-4522(95)00313-8

    authors: Ferrer I,Planas AM

    更新日期:1995-12-01 00:00:00

  • Involvement of Na+-Ca2+ exchanger on metabotropic glutamate receptor 1-mediated [Ca2+]i transients in rat cerebellar Purkinje neurons.

    abstract::Cerebellar Purkinje neurons have intracellular regulatory systems including Ca2+-binding proteins, intracellular Ca2+ stores, Ca2+-ATPase and Na+-Ca2+ exchanger (NCX) that keep intracellular Ca2+ concentration ([Ca2+]i) in physiological range. Among these, NCX interacts with AMPA receptors, activation of which induces...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/j.neuroscience.2007.01.040

    authors: Kim YT,Namkung YL,Kwak J,Suh CK

    更新日期:2007-04-25 00:00:00

  • Facial motor neuron regeneration induces a unique spatial and temporal pattern of myristoylated alanine-rich C kinase substrate expression.

    abstract::We have previously shown that the myristoylated alanine-rich C kinase substrate, a primary protein kinase C substrate in brain that binds and cross-links filamentous actin, is enriched in neuronal growth cones and is developmentally regulated in brain. Here we examined myristoylated alanine-rich C kinase substrate exp...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(00)00039-7

    authors: McNamara RK,Jiang Y,Streit WJ,Lenox RH

    更新日期:2000-01-01 00:00:00

  • Subthreshold membrane potential oscillations in neurons of deep layers of the entorhinal cortex.

    abstract::Neuronal oscillations are important for information processing. The entorhinal cortex is one of the structures which is involved in generation of theta rhythm. The major role of the entorhinal cortex is to feed diverse sources of information both to and from the hippocampus. Far from simply being a funnel for this inf...

    journal_title:Neuroscience

    pub_type: 杂志文章

    doi:10.1016/s0306-4522(98)00113-4

    authors: Schmitz D,Gloveli T,Behr J,Dugladze T,Heinemann U

    更新日期:1998-08-01 00:00:00