Dimethylformamide is not better than glycerol for cryopreservation of boar semen.

Abstract:

:To improve the boar sperm cryopreservation process, the influence of the sugar (lactose, trehalose) source and the cryoprotectant [glycerol, dimethylformamide (DMF)] on the success of freezing was investigated. Sperm samples were frozen in one of six extenders: lactose plus 3% glycerol (LG); lactose plus 1.5% glycerol and 1.5% DMF (LGD); lactose plus 3% DMF (LD); trehalose plus 3% glycerol (TG); trehalose plus 1.5% glycerol and 1.5% DMF (TGD); trehalose plus 3% DMF (TD). Effects on motility, viability, acrosome integrity and hypoosmotic test (HOST) were measured. The results showed that extender containing 3% glycerol retained the highest motility percentages. In regard to viability and acrosome integrity, all extenders yielded similar rates except for the decreasing values of TD. Endosmosis was diminished in TD and LD at 2 h (P = 0.0018), as compared with the others. The results of the study demonstrated that the use of DMF as a cryoprotectant adversely affected boar sperm quality after cryopreservation.

journal_name

Andrologia

journal_title

Andrologia

authors

Malo C,Gil L,Cano R,Martínez F,García A,Jerez RA

doi

10.1111/j.1439-0272.2011.01237.x

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

605-10

eissn

0303-4569

issn

1439-0272

journal_volume

44 Suppl 1

pub_type

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