Abstract:
PURPOSE:To determine whether gene expression changes occur in the trabecular meshwork (TM) of cow eyes with steroid-induced intraocular pressure (IOP) elevation. METHODS:Adult female Braford cows (n = 4) were subjected to uniocular prednisolone acetate treatment for 6 weeks. IOP was monitored with an applanation tonometer. At the conclusion of the experiment, animals were euthanized, eyes were enucleated, and the TM was dissected and stored in an aqueous nontoxic tissue storage reagent. RNA was extracted and subjected to microarray analysis using commercial oligonucleotide bovine arrays. Some of the genes differentially expressed between control and experimental eyes were confirmed by quantitative RT-PCR and some of the respective proteins were studied by immunoblotting. RESULTS:IOP began to increase after 3 weeks of treatment, reaching a peak 2 weeks later. IOP differences between corticosteroid-treated and fellow control eyes were 6 ± 1 mm Hg (mean ± SD) at the conclusion of the study. Microarray analysis revealed that expression of 258 genes was upregulated, whereas expression of 187 genes was downregulated in the TM of eyes with steroid-induced IOP elevation. Genes identified to be differentially expressed include genes coding for cytoskeletal proteins, enzymes, growth and transcription factors, as well as extracellular matrix proteins and immune response proteins. A number of relevant gene networks were detected by bioinformatic analysis. CONCLUSIONS:Steroid-induced IOP elevation alters gene expression in the bovine TM. Identification of genes with changing expression in this model of open-angle glaucoma may help elucidate the primary changes occurring at the molecular level in this condition.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Danias J,Gerometta R,Ge Y,Ren L,Panagis L,Mittag TW,Candia OA,Podos SMdoi
10.1167/iovs.11-7563subject
Has Abstractpub_date
2011-11-07 00:00:00pages
8636-45issue
12eissn
0146-0404issn
1552-5783pii
iovs.11-7563journal_volume
52pub_type
杂志文章abstract:PURPOSE:Rabbits are commonly used for the evaluation of drugs and surgery to lower intraocular pressure (IOP). The accuracy of intraocular pressure measurement is therefore critical in the analysis of data and subsequent extrapolation to humans. The purpose of this study was to establish the most reliable technique, fr...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0610
更新日期:2005-07-01 00:00:00
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更新日期:2010-08-01 00:00:00
abstract::Vitreous and macrophage samples were tested for the ability to stimulate proliferation and cell migration in cultured rabbit retinal pigment epithelium (RPE). A macrophage invasion was elicited by the intravitreal injection of latex particles in rabbits and after 3 days the vitreal macrophages were collected. The macr...
journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1987-07-01 00:00:00
abstract:PURPOSE:To evaluate the serum fatty acid changes in Chinese patients with Bietti crystalline dystrophy (BCD) in association with CYP4V2 mutation. METHODS:Sixteen Chinese patients with BCD confirmed with CYP4V2 mutation were recruited. Peripheral venous blood was obtained after fasting and serum fatty acid concentratio...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,评审
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1982-03-01 00:00:00
abstract:PURPOSE:To develop a sensitive and reproducible technique for measuring the adherence of blood lymphocytes to vessel walls exposed in sections of human retina and for examining the role of lymphocyte and vascular adhesion molecules in these events. METHODS:Cryostat sections of human retina were overlaid with blood lym...
journal_title:Investigative ophthalmology & visual science
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更新日期:1997-11-01 00:00:00
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更新日期:2009-04-01 00:00:00
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更新日期:2011-08-09 00:00:00
abstract::The monoclonal antibody GRC1 was obtained by immunizing BALB/c mice with human cornea. Screening was performed by indirect immunofluorescence in cryostatic sections of several tissues: cornea, skin, placenta, hyaline cartilage, blood vessels, and nerves. GRC1 was seen to recognize fibrillar structures in all of these ...
journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1993-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2010-05-01 00:00:00
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更新日期:2010-07-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2016-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Epidermal growth factor (EGF), which has been shown to stimulate epidermal proliferation and keratinization and to induce regeneration of rabbit corneal epithelium, was studied for its immunogenic potential in rabbits. Mouse-derived EGF was administered topically, subconjunctivally, intrastromally, and systemically. S...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
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更新日期:2003-01-01 00:00:00
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更新日期:2009-12-01 00:00:00
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更新日期:2017-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2000-07-01 00:00:00
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更新日期:2013-02-28 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2011-10-31 00:00:00
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更新日期:2017-01-01 00:00:00
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更新日期:2015-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
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更新日期:2011-07-23 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0677
更新日期:2005-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00