Abstract:
:Discovery of new central nervous system (CNS) acting therapeutics has been slowed down by the lack of useful applicable biomarkers of disease or drug action often due to inaccessibility of relevant human CNS tissue and cell types. In recent years, non-neuronal cells, such as astrocytes, have been reported to play a highly significant role in neurodegenerative diseases, CNS trauma, as well as psychiatric disease and have become a target for small molecule and biologic therapies. We report the development of a method for measuring pharmacodynamic changes induced by potential CNS therapeutics using nasal olfactory neural tissue biopsy. We validated this approach using a potential astrocyte-targeted therapeutic, thiamphenicol, in a pre-clinical rodent study as well as a phase 1 human trial. In both settings, analysis of the olfactory epithelial tissue revealed biological activity of thiamphenicol at the drug target, the excitatory amino acid transporter 2 (EAAT2). Therefore, this biomarker approach may provide a reliable evaluation of CNS glial-directed therapies and hopefully improve throughput for nervous system drug discovery.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Sattler R,Ayukawa Y,Coddington L,Sawa A,Block D,Chipkin R,Rothstein JDdoi
10.1016/j.expneurol.2011.09.002subject
Has Abstractpub_date
2011-12-01 00:00:00pages
203-11issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(11)00309-8journal_volume
232pub_type
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journal_title:Experimental neurology
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