Abstract:
:Techniques are described for visualizing intracellular tropoelastin at the light level using immunofluorescence and immunogold techniques. Best results were obtained with B5 fixative on cells permeabilized with acetone. Using either formaldehyde or paraformaldehyde for fixation (instead of B5) resulted in both less reproducible and less intense intracellular staining, and permeabilization of the cells with ethanol resulted in relatively high background staining compared with that obtained with cold (-20 degrees C) acetone. Intracellular tropoelastin was seen most prominently in the perinuclear region, and the intensity of staining agreed with the reported rate of tropoelastin synthesis as assayed by enzyme-linked immunosorbent assay (ELISA) and RNA hybridization studies. The applicability of the intracellular staining technique for studying the elastin phenotype was tested by demonstrating increases in both the number of positive cells and in the intensity of elastin staining in cells treated with smooth muscle elastogenic factor (SMEF), an elastogenic factor known to stimulate elastin production.
journal_name
Am J Respir Cell Mol Biolauthors
Grosso LE,Whitehouse LA,Mecham RPdoi
10.1165/ajrcmb/3.1.45subject
Has Abstractpub_date
1990-07-01 00:00:00pages
45-9issue
1eissn
1044-1549issn
1535-4989journal_volume
3pub_type
杂志文章abstract::Hyperplasia of airway smooth muscle cells is present in the airways of asthmatic patients and may contribute to the development of the bronchial hyperresponsiveness that occurs in these patients. Because tryptase is an abundant component of mast cell granules and has demonstrated growth-stimulatory effects in other me...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.13.2.7626290
更新日期:1995-08-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章,评审
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章,评审
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更新日期:2015-02-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2006-0040OC
更新日期:2006-12-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2012-0187OC
更新日期:2013-01-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1165/rcmb.2007-0004RC
更新日期:2007-07-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb/4.4.379
更新日期:1991-04-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.20.4.3481
更新日期:1999-04-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2017-0382OC
更新日期:2018-11-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2016-0077OC
更新日期:2016-10-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/rcmb.2015-0119OC
更新日期:2016-06-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.21.2.3605
更新日期:1999-08-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章,评审
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journal_title:American journal of respiratory cell and molecular biology
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更新日期:2010-08-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
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更新日期:2019-05-01 00:00:00
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journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb.14.4.8600940
更新日期:1996-04-01 00:00:00
abstract::T lymphocytes and alveolar macrophages accumulating in the lower respiratory tract of patients with pulmonary sarcoidosis are known to be activated to produce several cytokines, presumably leading to granuloma formation within the lung. We hypothesized that these cells produce colony-stimulating factors (CSFs), which ...
journal_title:American journal of respiratory cell and molecular biology
pub_type: 杂志文章
doi:10.1165/ajrcmb/3.3.245
更新日期:1990-09-01 00:00:00