Abstract:
BACKGROUND:Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. OBJECTIVE:To assess the value of multiplex PCR in identification of dermatophytes. METHODS:Using three specially-designed primers which contained the ITS1-2, 18S rRNA, and 28S rRNA regions, three cycles of PCR were performed on 11 standard strains and scales were collected from 73 patients with fungal infection. RESULTS:The 11 standard strains were successfully identified with analysis of band patterns of ITS1-2, 18S rRNA, and 28S rRNA, obtained from PCR. Based on this information, the causative organisms in 73 patients with fungal infection were revealed to be T. rubrum in 69 cases, T. menta in 1 case, T. tonsurans in 2 cases, and M. gypseum in one case. CONCLUSION:With three cycles of PCR using three sets of primers, 11 standard strains and the clinical strains from 73 patients with fungal infection were successfully identified.
journal_name
Ann Dermatoljournal_title
Annals of dermatologyauthors
Kim JY,Choe YB,Ahn KJ,Lee YWdoi
10.5021/ad.2011.23.3.304subject
Has Abstractpub_date
2011-08-01 00:00:00pages
304-12issue
3eissn
1013-9087issn
2005-3894journal_volume
23pub_type
杂志文章abstract::Entecavir (Baraclude®, Bristol-Myers Squibb) is a potent and selective antiviral agent that has demonstrated efficacy in patients with chronic hepatitis B. The most frequent adverse events attributed to entecavir include increased alanine aminotransferase, upper respiratory tract infection, headache, abdominal pain, c...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2013.25.4.493
更新日期:2013-11-01 00:00:00
abstract::Sweet's syndrome is a reactive dermatosis characterized clinically by fever, leukocytosis, and multiple, erythematous, painful plaques. Histopathologic examination reveals a band-like dense dermal inflammatory infiltrate composed mainly of neutrophils with papillary dermal edema, and no features of vasculitis. We repo...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2009.21.3.300
更新日期:2009-08-01 00:00:00
abstract::Basal cell carcinoma (BCC), which frequently occurs in sun-exposed areas of the head and neck region, is the most common cutaneous malignancy. The nipple-areola complex (NAC) is an uncommon site for BCC to develop. BCCs in this region display more aggressive behavior and a greater potential to spread than when found i...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2011.23.S2.S201
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:The use of growth factors in skin rejuvenation is emerging as a novel anti-aging treatment. While the role of growth factors in wound healing is well established, their use in skin rejuvenation has only recently been to be studied and no controlled trials have been performed. OBJECTIVE:We evaluated the anti...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2014.26.5.584
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Psoriasis is a common chronic inflammatory skin disease with a strong genetic basis. Cytokines such as tumor necrosis factor alpha (TNF-α), interleukins (ILs) such are IL-12 and IL-23, and interferon gamma (IFN-γ) are released from various inflammatory and resident cells, and have been implicated in the init...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2015.27.2.128
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:The treatment of post-inflammatory hyperpigmentation (PIH) remains challenging. Tranexamic acid, a well-known anti-fibrinolytic drug, has recently demonstrated a curative effect towards melasma and ultraviolet-induced PIH in Asian countries. However, the precise mechanism of its inhibitory effect on melanoge...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2015.27.3.250
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:Gray hair naturally develops in the process of human aging. Many people with gray hair periodically dye their hair. Hair dyeing products are widely used and they can cause adverse effects. Therefore, the user's knowledge and recognition about hair dyeing and related side effects are important. OBJECTIVE:The...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2012.24.3.274
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:Several workers have found that Malassezia are capable of suppressing cytokine release and downregulating the phagocytic function of monocytes. But lipid-depleted Malassezia furfur (M. furfur) extracts have also been shown to induce increased production of TNF-alpha, IL-6 and IL-1beta in monocytes. We though...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2009.21.2.130
更新日期:2009-05-01 00:00:00
abstract::Non-tubecrulosis mycobacterium infections were increasingly reported either pulmonary or extrapulmonary in the past decades. In Taiwan, we noticed several reports about the soft tissue infections caused by rapid growing mycobacterium such as Mycobacterium abscessus, Mycobacterium chelonae, on newspaper, magazines, or ...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2014.26.2.236
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Angiogenesis is crucial for wound healing and exogenous supplements of the angiogenic growth factors have been known to promote cutaneous wound healing. Angiopoietin (Ang) 1 is a recently discovered angiogenic factor and there have been few studies of its effect on cutaneous wound healing. OBJECTIVE:We exam...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2008.20.2.49
更新日期:2008-06-01 00:00:00
abstract::Multiple autoimmune syndrome (MAS) is a rare condition, in which at least three well-defined autoimmune diseases co-occur in a single patient. Although the pathogenesis of MAS remains poorly understood, genetic, immunological, hormonal, and environmental factors may all play a role. Although three classic subtypes of ...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2017.29.6.782
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Seborrheic keratosis (SK) is one of the most common epidermal tumors of the skin. However, only a few large-scale clinicohistopathological investigations have been conducted on SK or on the possible correlation between histopathological SK subtype and location. OBJECTIVE:The aim of this study was to analyze...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2016.28.2.152
更新日期:2016-04-01 00:00:00
abstract::We report here on an 11-year-old child with a pustulovesicular eruption on her whole body, including her palms, soles and oral mucosa, and this was accompanied with fever. A serologic test was positive for IgM varicella zoster antibody and coxsackievirus A16 antibody. The histopathologic examination from the palm reve...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2009.21.1.98
更新日期:2009-02-01 00:00:00
abstract::We report on a rare case of fluconazole induced fixed drug eruption in a 62-year old female patient. She was referred to our department for multiple erythematous itchy maculopatches on the face, neck, both upper arms, and trunk area, which had occurred over the previous 6 months. Her attending physician prescribed flu...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2011.23.S1.S1
更新日期:2011-09-01 00:00:00
abstract::Fexofenadine (Allegra® 180) is a second-generation antihistamine. It is widely used as anti-allergic drug, which suppresses various allergic reactions mediated by histamines. A few cases of H1-antihistamine-induced urticaria have been reported. Herein, we report a rare case of fexofenadine-induced urticaria which was ...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2011.23.S3.S329
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:The expression of c-Met is substantially elevated in most malignant human cancers. We therefore searched for c-Met expression and compared the expression level among malignant skin cancers. OBJECTIVE:The aim of this study was to determine the c-Met expression pattern and the protein expression level in sele...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2011.23.1.33
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:Several previous studies have suggested the improvement of atopic dermatitis (AD) in response to special fabrics. In particular, beneficial effects have been reported, following the use of anion textiles. OBJECTIVE:The purpose of this study is to evaluate the effectiveness and safety of an anion textile in ...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2012.24.4.438
更新日期:2012-11-01 00:00:00
abstract::Bowenoid papulosis (BP) is a disease that mainly occurs in sexually active young adults. The patients present with multiple pigmented papular lesions with a verrucous surface on the anogenital area. Although extragenital BP is usually associated with concomitant genital involvement, a few cases of isolated extragenita...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2014.26.3.381
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:The atopic dermatitis (AD) can limit a patient's physical and psychosocial development as well as lower their overall quality of life (QOL), including sleep quality. OBJECTIVE:The purpose of this study was to evaluate the relationships between clinical disease severity, QOL and sleep quality in children and...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2016.28.3.321
更新日期:2016-06-01 00:00:00
abstract::Angiolymphoid hyperplasia with eosinophilia (ALHE) is an uncommon dermal angioproliferating tumor, characterized by red to brown papules or nodules on the head and neck, though also occurring in the mouth, trunk, extremities and inguinal area. The palm is a very unusual site for ALHE, and there have been very few case...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2010.22.3.358
更新日期:2010-08-01 00:00:00
abstract::Epidermolysis bullosa (EB) is a rare hereditary disorder characterized by formation of blisters following minor trauma. It has been traditionally categorized by the level of basement membrane zone separation into EB simplex (EBS), junctional EB (JEB), and dystrophic EB (DEB). Recently, hemidesmosomal EB has been propo...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2011.23.S1.S41
更新日期:2011-09-01 00:00:00
abstract::Lymphomatoid keratosis (LK) is considered to be a rare variant of cutaneous lymphoid hyperplasia, with epidermotropism. We herein report a case of LK which developed on the abdomen of an elderly Korean woman. A 60-year-old woman presented with a 10-year history of a pruritic, solitary, brown to black plaque on the abd...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2010.22.2.219
更新日期:2010-05-01 00:00:00
abstract::The development of cutaneous sarcoidosis as a paradoxical adverse event of tumor necrosis factor alpha (TNF-α) blockers has been reported in the literature; however, an erythrodermic form of cutaneous sarcoidosis during anti-TNF-α therapy has not yet been reported. Herein, we report the first case of an erythrodermic ...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2017.29.1.74
更新日期:2017-02-01 00:00:00
abstract::[This corrects the article on p. 211 in vol. 30, PMID: 29606819.]. ...
journal_title:Annals of dermatology
pub_type: 已发布勘误
doi:10.5021/ad.2018.30.4.511
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:The extracellular matrix (ECM) produced by dermal fibroblasts supports skin structure, and degradation and/or reduced production of ECM are the main causes of wrinkle formation. OBJECTIVE:The aim of this study was to identify the active ingredient that enhances ECM production in dermal fibroblasts. METHODS...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2012.24.1.16
更新日期:2012-02-01 00:00:00
abstract::Touraine-Soulente-Golé Syndrome (TSG) or pachydermoperiostosis is a rare disorder characterized by pachydermia, periostosis & digital clubbing. Herein, we report a case of a 27 year old male, with the looks of a 47 year old. He presented with excessive wrinkling on his face since past 8 years. TSG syndrome was suspect...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2013.25.3.352
更新日期:2013-08-01 00:00:00
abstract::Patients with chronic lymphocytic leukemia (CLL) rarely exhibit an exaggerated insect bite-like reaction without a history of an arthropod bite. We report a case of an insect bite-like reaction in a 74-year old man with CLL. The patient presented with a 2-year history of recurrent itchy erythematous patches and bliste...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2018.30.4.468
更新日期:2018-08-01 00:00:00
abstract::Pityriasis versicolor is a superficial infection of the stratum corneum, which is caused by the Malassezia species. Tge Malassezia species consist of 12 subspecies, including M. furfur, M. pachydermatis, M. symphodialis and M. globasa. The Malassezia species are classified as a normal flora, particularly in the sebum ...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2012.24.3.345
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:Stress is a known cause of hair loss in many species. OBJECTIVE:In this study, we investigated the role of acute stress on hair growth using a rat model. METHODS:Rats were immobilized for 24 hours and blood samples, and skin biopsies were taken. The effect of stress-serum on the in vitro proliferation of r...
journal_title:Annals of dermatology
pub_type: 杂志文章
doi:10.5021/ad.2016.28.5.600
更新日期:2016-10-01 00:00:00
abstract::An atypical clinical form of pityriasis versicolor has been infrequently reported, in which cutaneous atrophy is associated with individual pityriasis versicolor lesions. The pathogenesis of this atrophy remains unclear, but is believed to be a delayed-type hypersensitivity reaction to antigens derived from the Malass...
journal_title:Annals of dermatology
pub_type:
doi:10.5021/ad.2010.22.4.456
更新日期:2010-11-01 00:00:00