Abstract:
:While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm.
journal_name
PLoS Pathogjournal_title
PLoS pathogensauthors
Absalon C,Van Dellen K,Watnick PIdoi
10.1371/journal.ppat.1002210subject
Has Abstractpub_date
2011-08-01 00:00:00pages
e1002210issue
8eissn
1553-7366issn
1553-7374pii
PPATHOGENS-D-11-00127journal_volume
7pub_type
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