Abstract:
:Yeast enolase is stabilized by its natural cofactor Mg(2+). This stabilization is ascribed to the reduced subunit dissociation of the holoprotein. Nevertheless, how Mg(2+) alters the unfolding mechanism has yet to be fully characterized. Here, we investigate the role of Mg(2+) in the denaturation mechanism and unfolding kinetics of yeast enolase. Apo-enolase unfolds through a three-state process (N(2)↔2I→2D). The intermediate species is described as a monomeric molten globule-like conformation that becomes noticeable in the presence of phosphate and is able to recover its native secondary structure when cooled down. Kinetic studies confirmed the presence of the intermediate species, even though it was not noticeable in the thermal scans. The cofactor increases the cooperativity of the unfolding transitions, while the intermediate species becomes less noticeable or nonexistent. Thus, holo-enolase follows a simple two-state mechanism (N(2)→2D). Our results indicate smaller unfolding rate-constants in the presence of Mg(2+), thus favoring the native state. The temperature dependence of the unfolding rates allowed us to calculate the activation enthalpies of denaturation. Interestingly, despite the different unfolding mechanisms of the apo and holo forms of enolase, they both have similar activation barriers of denaturation (185-190 kJ mol(-1)).
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Moreno-Vargas LM,Carrillo-Ibarra N,Arzeta-Pino L,Benítez-Cardoza CGdoi
10.1016/j.ijbiomac.2011.07.021subject
Has Abstractpub_date
2011-12-01 00:00:00pages
871-8issue
5eissn
0141-8130issn
1879-0003pii
S0141-8130(11)00299-6journal_volume
49pub_type
杂志文章abstract::Identifying protein-protein interfaces is crucial for structural biology. Because of the constraints in wet experiments, many computational methods have been proposed. Without knowing any information about the partner chains, a new method of predicting protein-protein interaction interface residues purely based on evo...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2006.02.024
更新日期:2006-05-30 00:00:00
abstract::We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro. The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjuga...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.054
更新日期:2018-08-01 00:00:00
abstract::Development of a gastroretentive sustained release tablet of metformin based on poly (acrylic acid)-grafted-gellan (PAAc-g-GG) is the main purpose of this study. At first, PAAc-g-GG was synthesized by microwave-promoted free radical initiation method using cerric (IV) ammonium nitrate (CAN) as redox initiator and char...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.12.022
更新日期:2017-03-01 00:00:00
abstract::Clinical application of curcumin for Alzheimer's disease treatment is severely limited with regard to its poor bioavailability, high rate of metabolism, and instability under neutral condition. In the current study, we designed three compounds in which the diketone moiety of curcumin was replaced by cyclohexanone. In ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.04.038
更新日期:2015-01-01 00:00:00
abstract::Opuntia is a traditional plant from China with medicinal applications. In this experiment, polysaccharides from Opuntia Milpa Alta (MAPs) were analyzed using gas chromatograph-mass spectrometer (GC-MS) method and result showed that MAPs contained mannose (6.37%), rhamnose (14.94%), xylose (1.99%), arabinose (24.07%), ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.06.031
更新日期:2011-11-01 00:00:00
abstract::Glycerol is an effective carbon source for the production of scl- and mcl-polyhydroxyalkanoates (PHAs) by Pseudomonas spp. P. mediterranea 9.1 (CFBP 5447) synthesizes an amorphous mcl-PHA when grown on crude glycerol, whereas on both reagent grade (RG) and partially refined (PR) glycerol, it produces two very similar ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.01.014
更新日期:2014-04-01 00:00:00
abstract::A bacterium capable of degrading poly(3-hydroxybutyrate) [P(3HB)] was isolated from laboratory media and identified as Pseudomonas pickettii. The strain P. pickettii excreted an extracellular poly(hydroxyalkanoate) (PHA) depolymerase and grew on P(3HB) as the sole carbon source. P. pickettii also grew on 3-hydroxybuty...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(93)90040-s
更新日期:1993-08-01 00:00:00
abstract::β-Lactoglobulin (β-LG) is the major constituent of whey food, which has been shown to interact with a wide range of aroma compounds. In the present work, a model aroma compound, β-ionone, is used to investigate the influence of aroma compounds on the urea-induced unfolding of β-LG at pH 7.0. β-Ionone is observed to en...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.12.003
更新日期:2014-03-01 00:00:00
abstract::Cordyceps cicadae is a traditional Chinese medicine with high nutritional value and biological activities. Previously, we reported on the antioxidant activity associated with the polysaccharides from Cordyceps cicadae (CP). To further explore which of the fraction of CP had the greatest potency, in here, the in vitro ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.04.163
更新日期:2020-08-15 00:00:00
abstract::Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.106
更新日期:2018-12-01 00:00:00
abstract::This study is the first to evaluate the effects of Iron oxides (FeOx) species and their decoration on graphene oxide/chitosan (GO/CS) composites for Cr(VI) removal and the possibility of Fe secondary pollution. Results show that Fe(III) is a better decoration material than Fe(II) and decoration through immersion-evapo...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2021.01.060
更新日期:2021-01-13 00:00:00
abstract::Coriolus versicolor is an edible medicinal mushroom in China. Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation. Their chemical structures were well elucidated with overall consideration of monosaccharide compo...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.11.018
更新日期:2021-01-01 00:00:00
abstract::Two diketone based oxovanadium complexes, viz., bis(4,4,4-trifluoro-1-phenylbutane-1,3-dionato)oxovanadium(IV) (1) and bis(1,1,1-trifluoropentane-2,4-dionato)oxovanadium(IV) (2), have been synthesized and characterized by spectroscopic and analytical techniques. The DNA binding and the cleaving ability of the complexe...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.02.027
更新日期:2015-05-01 00:00:00
abstract::A response surface methodology was used to optimize the parameters for extracting the polysaccharides from tobacco waste (TWPs) using hot water. The extraction process, carried out under the following optimized parameters: an extraction temperature of 90°C, a ratio of water to raw material of 54, and an extraction tim...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.05.069
更新日期:2016-10-01 00:00:00
abstract::Dilute solution properties of an unknown starch are important to understand its performance and applications in food and non-food industries. In this paper, rheological and molecular properties (intrinsic viscosity, molecular weight, shape factor, voluminosity, conformation and coil overlap parameters) of the starches...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.02.050
更新日期:2016-06-01 00:00:00
abstract::1,4-α-Glucan branching enzyme (GBE, EC. 2.4.1.18), which plays a key role in the synthesis of starch and glycogen, has been overexpressed in E. coli as an intracellular enzyme by many researchers. In this study, it was found that the GBEs from Geobacillus thermoglucosidans and Rhodothermus obamensis were secreted into...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.002
更新日期:2019-07-01 00:00:00
abstract::Effects of temperature and solvent condition on phase separation-induced molecular fractionation of gum arabic/hyaluronan (GA/HA) mixed solutions were investigated. Two gum arabic samples (EM10 and STD) with different molecular weights and polydispersity indices were used. Phase diagrams, including cloud and binodal c...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.05.073
更新日期:2018-09-01 00:00:00
abstract::The purpose of this investigation was to develop Poly (D,L Lactide-co-Glycolide) (PLGA)/poloxamer nanoparticles (NPs) of the hydrophilic drug Zolmitriptan using quality-by-design approach for brain targeting. Randomized 2(4) full factorial design was employed to achieve the critical quality attributes of minimized par...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.12.069
更新日期:2016-04-01 00:00:00
abstract::The essential amino acid l-tryptophan is naturally present in the body, and is also available as a water soluble dietary supplement. The feasibility of preparing enriched cellulose acetate (CA)-based fibres as a vehicle for therapeutic delivery of such biomolecules was investigated. A new ternary solvent system consis...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.11.124
更新日期:2018-03-01 00:00:00
abstract::Artemisinin, a natural anti-malarial agent, also possesses anti-proliferative and anti-angiogenic activity in cancer cells with very low toxicity to normal healthy cells. Drug loaded magnetic nanoparticles by using external magnetic field could selectively accumulate the drug at the target site and thereby reduce the ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.137
更新日期:2017-11-01 00:00:00
abstract::The so-called 'H-fragment' of insulin is an extremely amyloidogenic double chain peptide consisting of the N-terminal parts of A-chain and B-chain linked by a disulfide bond between Cys-7A and Cys-7B. Here, we conduct a detailed investigation of the self-association behavior of H-fragment monomers into amyloid-like fi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.02.153
更新日期:2020-05-01 00:00:00
abstract::We have reported the studies related to the fabrication of a nanocomposite, comprising of sol-gel derived inorganic zirconium oxide nanoparticles (ZrO2 NPs) and organic biopolymer gelatin-A (GA), deposited on indium-tin-oxide (ITO) coated glass substrate by drop casting method. The GA-ZrO2/ITO electrode was used for i...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.10.074
更新日期:2016-01-01 00:00:00
abstract::Glycation of biological macromolecule leads to the establishment of advanced glycation end products (AGEs) having implications in metabolic disorders. d‑ribose appears to be the most reactive among the naturally occurring sugars and contribute significantly to the glycation reactions in vivo, however, no report have b...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.01.161
更新日期:2018-06-01 00:00:00
abstract::The aim of the present work was to evaluate the performance of polypropylene (PP)/sisal fiber (SF)/banana fiber (BF) and chitosan-based hybrid (chitosan(CS)/SF)/BF) composite materials for the adsorptive removal of cadmium (Cd) ions from water waste. Polypropylene is harnessed for its importance in forming strong comp...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.07.252
更新日期:2020-12-01 00:00:00
abstract::Cellulose acetate nanofibrous mats were prepared by electrospunning technique. Positively charged and negatively charged composites were alternately deposited on negatively charged CA mats via layer-by-layer self-assembly technique. The morphology and inhibition rate of samples were investigated by regulating number o...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.10.024
更新日期:2013-02-01 00:00:00
abstract::Using high pressure microfluidization, we prepared micro-fibrillated soybean cellulose (MFSC) and analyzed its morphology and structure. MFSC was then incorporated into low-methoxyl pectin (PC) to coat lactic acid bacteria (LAB) by ionotropic gelation, and the effects of PC-MFSC microgel on LAB survival in a simulated...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.04.161
更新日期:2020-05-06 00:00:00
abstract::Serine protease inhibitors, inhibit serine proteases either partially or completely after forming complexes with their respective proteases. Protease actions are significant for many physiological pathways found in living forms and any anomalies may lead to numerous physiological complications. Each cell or organism h...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2017.09.115
更新日期:2018-02-01 00:00:00
abstract::Bicalutamide (BCT), a drug used in the treatment of prostate cancer, antagonises the actions of androgens, at the receptor level, thereby inhibiting the growth of prostate tumours. Alpha-2-macroglobulin (α2M), a pan-proteinase inhibitor, inhibits proteinase, regardless of specificity and catalytic mechanism. α2M is de...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.117
更新日期:2018-12-01 00:00:00
abstract::An unidentified Bacillus licheniformis trehalose-6-phosphate hydrolase (BlTreA) gene was cloned and heterologously expressed in Escherichia coli M15 cells. The over-expressed BlTreA was purified to apparent homogeneity by metal-affinity chromatography and its molecular mass was determined to be approximately 65.9 kDa....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.01.011
更新日期:2012-04-01 00:00:00
abstract::The present research investigates on task-specific deep eutectic solvents (TDES) based aqueous two-phase extraction and purification of immunoglobulins by chromatography from quail egg. The synthesis of TDES was accomplished with quaternary ammonium salt as hydrogen bond acceptor (HBA) and glycerol as hydrogen bond do...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.12.242
更新日期:2020-03-01 00:00:00