Abstract:
:In this study, the complementary DNA (cDNA) of Japanese pufferfish Takifugu rubripes tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) was cloned by reverse-transcription PCR. The open reading frame of the TRAIL consisted of 870 bases. The deduced amino-acid sequence of the TRAIL showed a high homology with the sequences of other teleosts. Recombinant soluble TRAIL was fused with a small ubiquitin-related modifier gene to enhance the soluble expression level in Escherichia coli BL21 (DE3). In vitro, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide (MTT) assay indicated that the purified soluble TRAIL was able to induce apoptosis of Jurkat and HeLa cells in a dose-dependent manner.
journal_name
J Fish Bioljournal_title
Journal of fish biologyauthors
Li JF,Ai HX,Zhang J,Du MX,Zhang Z,Zhang JX,Zhang SQdoi
10.1111/j.1095-8649.2011.03058.xsubject
Has Abstractpub_date
2011-09-01 00:00:00pages
747-60issue
3eissn
0022-1112issn
1095-8649journal_volume
79pub_type
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