LC-ESI-MS characterisation of phytoalexins induced in chickpea and pea tissues in response to a biotic elicitor of Hypnea musciformis (red algae).

Abstract:

:A simple extraction procedure and HPLC method was developed to analyse the major and minor components of induced phytoalexins of elicited tissues (seeds) of chickpeas (Cicer arietinum L.) and peas (Pisum sativum L.) treated with a biotic elicitor (k-carrageenan) of Hypnea musciformis (red algae) from the Karachi coast. The level and timing of the induced phytoalexin production were estimated on the basis of various elicitor dilutions and as a function of time; the results are presented and discussed. A LC-ESI-MS/MS technique has been employed for the detection and characterisation of the induced phytochemical components (flavonoids and their glyco-conjugates). Nine flavonoids were identified from chickpeas: naringin, naringin malonate, liquiritigenin, naringenin, biochanin A, daidzein, formononetin, maackiain and medicarpin, while five flavonoids were identified from peas: afrormosin, anhydropisatin, pisatin, pseudobaptigenin and maackiain. These compounds play a vital role as phytoalexins because of their antimicrobial activity.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Arman M

doi

10.1080/14786419.2011.553952

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

1352-60

issue

14

eissn

1478-6419

issn

1478-6427

journal_volume

25

pub_type

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