Abstract:
:cDNAs encoding a delayed-rectifier-type K+ channel were cloned from both neonatal rat heart and ovariectomized, diethylstilbestrol-primed rat uterus by using the polymerase chain reaction. Both clones have nucleotide sequences identical to that encoding the rat kidney IsK channel [Takumi, T., Ohkubo, H. & Nakanishi, S. (1988) Science 242, 1042-1045] and encode a putative protein of 130 amino acids. Injection of RNA transcripts of the cDNAs into Xenopus oocytes resulted in the expression of a slowly activating, voltage-dependent K+ current. An antisense oligonucleotide, derived from the sequence of the clone, specifically inhibited the expression of the slow, outward current observed in cells injected with mRNAs isolated from the parent tissues (i.e., kidney, heart, and uterus), indicating that the cloned gene underlies the major K+ current expressed from RNA isolated from these tissues.
journal_name
Proc Natl Acad Sci U S Aauthors
Folander K,Smith JS,Antanavage J,Bennett C,Stein RB,Swanson Rdoi
10.1073/pnas.87.8.2975subject
Has Abstractpub_date
1990-04-01 00:00:00pages
2975-9issue
8eissn
0027-8424issn
1091-6490journal_volume
87pub_type
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更新日期:2010-12-14 00:00:00