Wild-type p53 controls cell motility and invasion by dual regulation of MET expression.

abstract：:Proteins, nucleic acids, and fluorescein-conjugated antibody are shown to be identifidable in situ via the fluorescence excited by the focused electron beam of a canning electron microscope. A molecular species is identified by its characteristic fluorescence spectrum and by a characteristic alteration of the spectrum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hough PV,McKinney WR,Ledbeter MC,Pollack RE,Moos HW

更新日期：1976-02-01 00:00:00

abstract：:Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schuster H,Mikolajczyk M,Rohrschneider J,Geschke B

更新日期：1975-10-01 00:00:00

abstract：:Some dinosaurs reached masses that were approximately 8 times those of the largest, ecologically equivalent terrestrial mammals. The factors most responsible for setting the maximal body size of vertebrates are resource quality and quantity, as modified by the mobility of the consumer, and the vertebrate's rate of ene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McNab BK

更新日期：2009-07-21 00:00:00

abstract：:Osmotic homeostasis in the brain involves movement of water through aquaporin-4 (AQP4) membrane channels. Perivascular astrocyte end-feet contain distinctive orthogonal lattices (square arrays) assembled from 4- to 6-nm intramembrane particles (IMPs) corresponding to individual AQP4 tetramers. Two isoforms of AQP4 res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furman CS,Gorelick-Feldman DA,Davidson KG,Yasumura T,Neely JD,Agre P,Rash JE

更新日期：2003-11-11 00:00:00

abstract：:Class Ib Qa-2 molecules are expressed in tissue culture cells as approximately 40-kDa membrane-bound, glycophosphatidylinositol-linked antigens and as approximately 39-kDa soluble polypeptides. Recently, alternative splicing events which delete exon 5 from a portion of Qa-2 transcripts were demonstrated to give rise t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tabaczewski P,Shirwan H,Lewis K,Stroynowski I

更新日期：1994-03-01 00:00:00

abstract：:Type 1 diabetes (T1D) is an autoimmune disease characterized by insulitis and islet β-cell loss. Thus, an effective therapy may require β-cell restoration and immune suppression. Currently, there is no treatment that can achieve both goals efficiently. We report here that GABA exerts antidiabetic effects by acting on ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soltani N,Qiu H,Aleksic M,Glinka Y,Zhao F,Liu R,Li Y,Zhang N,Chakrabarti R,Ng T,Jin T,Zhang H,Lu WY,Feng ZP,Prud'homme GJ,Wang Q

更新日期：2011-07-12 00:00:00

abstract：:To determine the importance of mitochondrial reactive oxygen species toxicity in aging and senescence, we analyzed changes in mitochondrial function with age in mice with partial or complete deficiencies in the mitochondrial antioxidant enzyme manganese superoxide dismutase (MnSOD). Liver mitochondria from homozygous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kokoszka JE,Coskun P,Esposito LA,Wallace DC

更新日期：2001-02-27 00:00:00

abstract：:We describe a synthetic ligand, "DAKLI" (Dynorphin A-analogue Kappa LIgand), related to the opioid peptide dynorphin A. A single reactive amino group at the extended carboxyl terminus permits various reporter groups to be attached, such as 125I-labeled Bolton-Hunter reagent, fluorescein isothiocyanate, or biotin. Thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein A,Nestor JJ Jr,Naidu A,Newman SR

更新日期：1988-10-01 00:00:00

abstract：:Natural environments are filled with multiple, often competing, signals. In contrast, biological systems are often studied in "well-controlled" environments where only a single input is varied, potentially missing important interactions between signals. Catabolite repression of galactose by glucose is one of the best-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Escalante-Chong R,Savir Y,Carroll SM,Ingraham JB,Wang J,Marx CJ,Springer M

更新日期：2015-02-03 00:00:00

abstract：:Recent findings suggest that neurons can efficiently repair oxidatively damaged DNA, and that both DNA damage and repair are enhanced by activation of excitatory glutamate receptors. However, in pathological conditions such as ischemic stroke, excessive DNA damage can trigger the death of neurons. Oxidative DNA damage...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Canugovi C,Yoon JS,Feldman NH,Croteau DL,Mattson MP,Bohr VA

更新日期：2012-09-11 00:00:00

abstract：:The tumor microenvironment restrains conventional T cell (Tconv) activation while facilitating the expansion of Tregs. Here we showed that Tregs' advantage in the tumor milieu relies on supplemental energetic routes involving lipid metabolism. In murine models, tumor-infiltrating Tregs displayed intracellular lipid ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pacella I,Procaccini C,Focaccetti C,Miacci S,Timperi E,Faicchia D,Severa M,Rizzo F,Coccia EM,Bonacina F,Mitro N,Norata GD,Rossetti G,Ranzani V,Pagani M,Giorda E,Wei Y,Matarese G,Barnaba V,Piconese S

更新日期：2018-07-10 00:00:00

abstract：:A 600-base-pair region essential for ColE1 and pMBl plasmid replication contains two promoters responsible for the synthesis of two RNA molecules central to copy number control. One promoter directs synthesis of the primer RNA precursor. The second promoter directs the synthesis of a small RNA molecule, RNAl, which ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cesareni G,Muesing MA,Polisky B

更新日期：1982-10-01 00:00:00

abstract：:An antibody (anti-p21ser) was raised against a ras p21-related synthetic peptide and was able to recognize specifically the substitution of serine for glycine at amino acid 12 of p21. This substitution causes oncogenic activation of p21. Anti-p21ser was found to immunoprecipitate v-Ki-ras p21 and to strongly inhibit i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark R,Wong G,Arnheim N,Nitecki D,McCormick F

更新日期：1985-08-01 00:00:00

abstract：:Oxidative stress exacerbates neovascularization (NV) in many disease processes. In this study we investigated the mechanism of that effect. Mice deficient in superoxide dismutase 1 (Sod1(-/-) mice) have increased oxidative stress and show severe ocular NV that is reduced to baseline by antioxidants. Compared with wild...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong A,Shen J,Zeng M,Campochiaro PA

更新日期：2011-08-30 00:00:00

abstract：:The U.S. case against Iran is based on Iran's deceptions regarding nuclear weapons development. This case is buttressed by assertions that a state so petroleum-rich cannot need nuclear power to preserve exports, as Iran claims. The U.S. infers, therefore, that Iran's entire nuclear technology program must pertain to w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stern R

更新日期：2007-01-02 00:00:00

abstract：:Steroidogenic acute regulatory protein (StAR) appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. cDNAs encoding StAR were isolated from a human adrenal cortex library. Human StAR, coexpressed in COS-1 cells with cytochrome P450scc and adrenodoxin, increased pregnenolone synt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugawara T,Holt JA,Driscoll D,Strauss JF 3rd,Lin D,Miller WL,Patterson D,Clancy KP,Hart IM,Clark BJ

更新日期：1995-05-23 00:00:00

abstract：:Despite extensive studies, the structural basis for the mechanochemical coupling in the rotary molecular motor F1-ATPase (F1) is still incomplete. We performed single-molecule FRET measurements to monitor conformational changes in the stator ring-α3β3, while simultaneously monitoring rotations of the central shaft-γ. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugawa M,Okazaki K,Kobayashi M,Matsui T,Hummer G,Masaike T,Nishizaka T

更新日期：2016-05-24 00:00:00

abstract：:In enteric bacteria, the cellular response to oxidative stress is activated by oxidation of the iron-sulfur clusters in SoxR, which then induces transcription of soxS, turning on a battery of defense genes. Here we demonstrate both in vitro and in cells that activation of SoxR can occur in a DNA-mediated reaction with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee PE,Demple B,Barton JK

更新日期：2009-08-11 00:00:00

abstract：:Treatment with neuraminidase (NA) plus galactose oxidase (GalOxase) does not cause stimulation of human thymocytes. However, stimulation can be achieved by addition of exogenous interleukin 2 (IL-2). The IL-2-induced stimulation was inhibited with anti-Tac antibody, indicating that NA/GalOxase-oxidized cells can serve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roffman E,Sredni B,Smolinsky A,Wilchek M

更新日期：1984-08-01 00:00:00

abstract：:Hepatocellular injury during hepatitis B virus (HBV) infection has been postulated to result from a human leukocyte antigen (HLA)-restricted T-lymphocyte host immune response against HBV antigens. Although HLA expression is enhanced in the presence of hepatic inflammation, whether HBV itself can induce HLA expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu KQ,Vierling JM,Siddiqui A

更新日期：1990-09-01 00:00:00

abstract：:Bacterial transpeptidase and transglycosylase on the surface are essential for cell wall synthesis, and many antibiotics have been developed to target the transpeptidase; however, the problem of antibiotic resistance has arisen and caused a major threat in bacterial infection. The transglycosylase has been considered ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang CY,Shih HW,Lin LY,Tien YW,Cheng TJ,Cheng WC,Wong CH,Ma C

更新日期：2012-04-24 00:00:00

abstract：:Humans reason about the mental states of others; this capacity is called Theory of Mind (ToM). In typically developing adults, ToM is supported by a consistent group of brain regions: the bilateral temporoparietal junction (TPJ), medial prefrontal cortex (MPFC), precuneus (PC), and anterior temporal sulci (aSTS). How ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bedny M,Pascual-Leone A,Saxe RR

更新日期：2009-07-07 00:00:00

abstract：:In feeding, aphids inject saliva into plant tissues, gaining access to phloem sap and eliciting (and sometimes overcoming) plant responses. We are examining the involvement, in this aphid-plant interaction, of individual aphid proteins and enzymes, as identified in a salivary gland cDNA library. Here, we focus on a sa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mutti NS,Louis J,Pappan LK,Pappan K,Begum K,Chen MS,Park Y,Dittmer N,Marshall J,Reese JC,Reeck GR

更新日期：2008-07-22 00:00:00

abstract：:Genes that are up- and down-regulated by thyroid hormone in the tail resorption program of Xenopus laevis have been isolated by a gene expression screen, sequenced, and identified in the GenBank data base. The entire program is estimated to consist of fewer than 35 up-regulated and fewer than 10 down-regulated genes; ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown DD,Wang Z,Furlow JD,Kanamori A,Schwartzman RA,Remo BF,Pinder A

更新日期：1996-03-05 00:00:00

abstract：:Domestic animals can be cloned using techniques such as embryo splitting and nuclear transfer to produce genetically identical individuals. Although embryo splitting is limited to the production of only a few identical individuals, nuclear transfer of donor nuclei into recipient oocytes, whose own nuclear DNA has been...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keefer CL

更新日期：2015-07-21 00:00:00

abstract：:Lizards and snakes putatively arose between the early Jurassic and late Triassic; they diversified worldwide and now occupy many different ecological niches, making them ideal for testing theories on the origin of ecological traits. We propose and test the "deep history hypothesis," which claims that differences in ec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vitt LJ,Pianka ER

更新日期：2005-05-31 00:00:00

abstract：:Formulas are derived for the effect of size on a free-swimming microbe's ability to follow chemical, light, or temperature stimuli or to disperse in random directions. The four main assumptions are as follows: (i) the organisms can be modeled as spheres, (ii) the power available to the organism for swimming is proport...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dusenbery DB

更新日期：1997-09-30 00:00:00

abstract：:Recombinant human erythropoietin (rHuEpo) has been used successfully in the treatment of cancer-related anemia. Clinical observations with several patients with multiple-myeloma treated with rHuEpo has shown, in addition to the improved quality of life, a longer survival than expected, considering the poor prognostic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mittelman M,Neumann D,Peled A,Kanter P,Haran-Ghera N

更新日期：2001-04-24 00:00:00

abstract：:Whether mitotic structures like the centrosome can self-organize from the regulated mobility of their dynamic protein components remains unclear. Here, we combine fluorescence spectroscopy and chemical genetics to study in living cells the diffusion of polo-like kinase 1 (PLK1), an enzyme critical for centrosome matur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahen R,Jeyasekharan AD,Barry NP,Venkitaraman AR

更新日期：2011-05-31 00:00:00

abstract：:The homotypic fusion of endoplasmic reticulum (ER) membranes is mediated by atlastin (ATL), which consists of an N-terminal cytosolic domain containing a GTPase module and a three-helix bundle followed by two transmembrane (TM) segments and a C-terminal tail (CT). Fusion depends on a GTP hydrolysis-induced conformatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu TY,Bian X,Sun S,Hu X,Klemm RW,Prinz WA,Rapoport TA,Hu J

更新日期：2012-08-07 00:00:00