Abstract：
:Anandamide (AEA), an endogenous cannabinoid and vanilloid receptor ligand, possesses anti-inflammatory properties. Transport of AEA through cytoplasm is facilitated by heat shock protein (HSP) Hsp70, which enhances the rate of cellular AEA uptake, possibly via direct interactions. In lungs, increased HSP expression is an endogenous, protective mechanism against acute lung inflammation. We hypothesised that AEA could enhance the expression of cytoprotective Hsp70 and Hsp25. Anaesthetised rats were injected intravenously with 1mg/kg AEA or saline. Lungs were removed 2 and 24 h after injection for evaluation of HSP expression. Hsp70 and Hsp25 expression in lungs was evaluated by immunohistochemistry. The relative levels of these HSPs in lung sections were determined through optical density measurements and western blotting of lung homogenates. Western blot and immunohistochemistry analyses indicated that expression of both proteins was significantly higher in AEA-injected animals than in control animals 2 and 24 h after treatment. AEA administration enhanced Hsp70 and Hsp25 expression in lungs. Therefore, AEA-HSP interactions could be involved in mechanisms protecting against lung inflammation, indicating a possible use of AEA as a treatment for lung inflammation.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Kopczyńska B,Sulejczak D,Wełniak-Kamińska M,Gietka A,Grieb Pdoi
10.1016/j.ejphar.2011.06.045subject
Has Abstractpub_date
2011-10-01 00:00:00pages
257-63issue
1-2eissn
0014-2999issn
1879-0712pii
S0014-2999(11)00752-7journal_volume
668pub_type
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