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Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes.

Abstract：

OBJECTIVE:To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium. DESIGN:Prospective, randomized study. SETTING:University research laboratory-based assisted reproductive technology laboratory. PATIENT(S):Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S):In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0-1, 2-3, 4-5, and 8-9 hours). Oocytes spindles in each group were immunostained for α-tubulin and chromosomes, and observed by confocal microscopy. MAIN OUTCOME MEASURE(S):Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly. RESULT(S):There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups. CONCLUSION(S):Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Yu Y,Yan J,Liu ZC,Yan LY,Li M,Zhou Q,Qiao J

doi

10.1016/j.fertnstert.2011.04.077

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

73-78.e1

issue

1

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(11)00690-X

journal_volume

96

pub_type

杂志文章,随机对照试验

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