Abstract:
:Rhagomys rufescens (Rodentia: Sigmodontinae) is an endemic species of the Atlantic forest from Southern and Southeastern Brazil. Some authors consider Rhagomys as part of the tribe Thomasomyini; but its phylogenetic relationships remain unclear. Chromosomal studies on eight specimens of Rhagomys rufescens revealed a diploid number of 2n = 36 and a number of autosome arms FN = 50. GTG, CBG and Ag-NOR banding and CMA(3) /DAPI staining were performed on metaphase chromosomes. Eight biarmed and nine acrocentric pairs were found in the karyotype of this species. The X and Y chromosomes were both acrocentric. Most of the autosomes and the sex chromosomes showed positive C-bands in the pericentromeric region. The X chromosome showed an additional heterochromatic block in the proximal region of the long arm. Nucleolus organizer regions (NORs) were located in the pericentromeric region of three biarmed autosomes (pairs 4, 6 and 8) and in the telomeric region of the short arm of three acrocentrics (pairs 10, 12 and 17). CMA (3) /DAPI staining produced fluorescent signals in many autosomes, especially in pairs 4, 6, and 8. This study presents cytogenetic data of Rhagomys rufescens for the first time.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Testoni AF,Althoff SL,Nascimento AP,Steiner-Souza F,Sbalqueiro IJdoi
10.1590/S1415-47572010005000071subject
Has Abstractpub_date
2010-07-01 00:00:00pages
479-85issue
3eissn
1415-4757issn
1678-4685journal_volume
33pub_type
杂志文章abstract::MicroRNAs are considered as potential biomarkers, agents, or therapeutic targets; few studies have addressed the expression of miRNAs in treatment-naïve patients infected with HIV-1. The aim of this study was to assess plasma relative circulating miRNA expression profiles in treatment-naïve Mexican patients with HIV/A...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2020-0017
更新日期:2020-06-22 00:00:00
abstract::Birth defects (BDs) are functional and structural alterations in embryonic or fetal development. With an incidence of approximately 3-5%, BDs are a leading cause of infant mortality and lifelong disability. A population-based prospective case-control study was conducted for one year with 5204 infants, between March 1s...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0186
更新日期:2020-02-10 00:00:00
abstract::Metachromatic leukodystrophy disorder (MLD) is an autosomal recessive and lysosomal storage disease. The disease is caused by the deficiency of the enzyme arylsulfatase A (ARSA) which is encoded by the ARSA gene. Different mutations have been reported in different populations. The present study was aimed to detect the...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0110
更新日期:2017-10-01 00:00:00
abstract::Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738120140162
更新日期:2015-03-01 00:00:00
abstract::A detailed study of putative recombination events and their evolution frequency in the whole genome of the currently known members of the family Tombusviridae, comprising 79 accessions retrieved from the international databases, was carried out by using the RECCO and RDP version 3.31β algorithms. The first program all...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011005000046
更新日期:2011-10-01 00:00:00
abstract::Catalase (CAT) is a common antioxidant enzyme in almost all living organisms. Currently, detailed reports on cucumber (Cucumis sativus L.) CAT (CsCAT) genes and tissue expression profiling are limited. In the present study, four candidate CsCAT genes were identified in cucumber. Phylogenetic analysis indicated that Cs...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2015-0192
更新日期:2016-07-01 00:00:00
abstract::The Latin American School of Human and Medical Genetics (ELAG) is the main course of its kind in the genetics field in Latin America. Here we describe the main challenges regarding the organization of such event, including how we obtain funding and how we proceed with student selection. Thus, we aim to share our exper...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572014000200002
更新日期:2014-03-01 00:00:00
abstract::Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738420150097
更新日期:2015-12-01 00:00:00
abstract::SPLs are plant-specific transcription factors that play important regulatory roles in plant growth and development. Systematic analysis of the SPL family has been performed in numerous plants, such as Arabidopsis, rice, and Populus. However, no comparative analysis has been performed across different species to examin...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2017-0144
更新日期:2019-04-01 00:00:00
abstract::Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previous...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-475738320140376
更新日期:2015-07-01 00:00:00
abstract::The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorph...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000041
更新日期:2010-04-01 00:00:00
abstract::Switchgrass (Panicum virgatum L.; family Poaceae) is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, incl...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2014-0300
更新日期:2016-03-01 00:00:00
abstract::Oral Lichen Planus (OLP) is an oral inflammatory condition, mediated by host immune system reaction, presenting basal membrane damages with inflammatory lesions in the mouth and/or skin. In this study, the role of functional polymorphisms in the MBL2 gene, encoding for Mannose-Binding Protein C (MBP-C), a member of th...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0015
更新日期:2019-01-01 00:00:00
abstract::The function of gene body DNA methylation in alternative splicing, and its relation to disease pathogenesis is not fully elucidated. The gene for familial Mediterranean fever (MEFV) encodes the pyrin protein and contains a 998 bp CpG island, covering the second exon, which is differentially methylated in FMF patients ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0234
更新日期:2017-07-01 00:00:00
abstract::The aim of this study was to estimate the components of variance and genetic parameters for the visual scores which constitute the Morphological Evaluation System (MES), such as body structure (S), precocity (P) and musculature (M) in Nellore beef-cattle at the weaning and yearling stages, by using threshold Bayesian ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000066
更新日期:2009-10-01 00:00:00
abstract::The antitumorigenic potential of two palladium(II) complexes, [Pd(ca(2)-o-phen)Cl(2)] - C1 and [Pd(dmba)(dppp)Cl] - C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER-). Growth inhibition and induced alterations in cell-morphology were a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572012005000016
更新日期:2012-01-01 00:00:00
abstract::Phylogenetic species of Paracoccidioides brasiliensis complex (S1a and S1b, PS2, PS3, and PS4) and Paracoccidioides lutzii are agents of paracoccidioidomycosis, an endemic fungal disease in Latin America. P. restrepiensis (PS3 genotype) was classified as monophyletic and geographically restricted to Colombia and neigh...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2019-0201
更新日期:2020-05-29 00:00:00
abstract::Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with l...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2016-0312
更新日期:2017-07-01 00:00:00
abstract::Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000038
更新日期:2010-04-01 00:00:00
abstract::B chromosomes are supernumerary elements present in about 15% of eukaryotic species and are most frequently heterochromatic, behave parasitically, show a transmission rate higher than standard (A) chromosomes, and can provoke harmful effects on carriers. In the current work, Prochilodus lineatus individuals carrying e...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572011005000009
更新日期:2011-04-01 00:00:00
abstract::We genotyped 15 microsatellite loci in order to evaluate the effects of habitat fragmentation, caused by flooding of the Tucuruí reservoir, on the genetic structure of Alouatta belzebul in eastern Amazonia. The analysis included two populations sampled in 1984, representing both margins of the Tocantins river, and thr...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/s1415-47572010000400027
更新日期:2010-10-01 00:00:00
abstract::The potential effect of electromagnetic fields (EMFs) emitted from video display terminals (VDTs) to elicit biological response is a major concern for the public. The software professionals are subjected to cumulative EMFs in their occupational environments. This study was undertaken to evaluate DNA damage and inciden...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000010
更新日期:2010-01-01 00:00:00
abstract::Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A t...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000038
更新日期:2009-04-01 00:00:00
abstract::Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We inve...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572009005000107
更新日期:2010-01-01 00:00:00
abstract::Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids a...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572010005000088
更新日期:2010-10-01 00:00:00
abstract::Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012000200016
更新日期:2012-06-01 00:00:00
abstract::Phylogenetic niche conservatism can be investigated at multiple scales on an explicit geographical context. Haplotype-based comparative analyses of lineages occupying the same region, and thus subjected to similar environmental factors, allow decoupling shared evolutionary and ecological patterns, as well as multiple ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2018-0291
更新日期:2020-04-27 00:00:00
abstract::Hereditary cancer risk assessment is a multidisciplinary and dynamic process, with the purpose of estimating probabilities of germline mutations in cancer susceptibility genes and assessing empiric risks of cancer based on personal and family histories, in order to offer clinical and molecular diagnoses and clinical m...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/1678-4685-GMB-2014-0346
更新日期:2016-05-13 00:00:00
abstract::Tabebuia sp. is native to tropical rain forests throughout Central and South America. Although the biological and pharmacological effects of bark extracts have been intensely studied, little is known on the extract obtained from the flower. Herein, the genotoxic potential of a flower extract from T. impetiginosa ("ipê...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572012005000030
更新日期:2012-04-01 00:00:00
abstract::The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, ...
journal_title:Genetics and molecular biology
pub_type: 杂志文章
doi:10.1590/S1415-47572011000100007
更新日期:2011-01-01 00:00:00