Osteogenic differentiation is synergistically influenced by osteoinductive treatment and direct cell-cell contact between murine osteoblasts and mesenchymal stem cells.

Abstract:

PURPOSE:The present study was designed to address whether osteoblasts play a synergistic role in promoting mesenchymal stem cell (MSC) osteogenesis in a direct cell-cell contact co-culture model. METHODS:Murine C3H10T1/2 and MC3T3-E1 cell lines were mixed and plated onto 12-well culture plates and co-cultured at various ratios of initial cell densities. To compare the possible improvement on osteogenic differentiation, co-culture cells were served with or without osteogenic supplements in culture medium. RESULTS:Weak osteogenesis was induced in MSCs co-cultured in an untreated medium with different ratios of osteoblasts. An osteoblast-dependent increase in osteogenic gene expression of Runx2, type I collagen, and osteocalcin was observed over time. Moreover, both alkaline phosphatase (ALP) activity and calcium deposition were distinctly enhanced at levels that were proportional to the quantity of osteoblasts in the culture. The increases in mRNA expression and ALP activity were greater in co-cultures treated with osteogenic supplements than in untreated cultures. However, the production of ALP activity followed by a distinct matrix mineralization was lower in osteogenic-treated cultures containing greater numbers of osteoblasts. This suggests that a higher density of osteoblasts may lead to weak osteogenesis of MSCs by direct cell-cell contact co-culture in an untreated environment. Furthermore, additional osteogenic supplements may act synergistically with osteoblasts to accelerate matrix mineralization by reducing the process of osteogenic differentiation in osteogenic treated co-cultures. CONCLUSIONS:The present work may improve the understanding of MSC osteogenesis and may provide benefits for regenerative medicine.

journal_name

Int Orthop

authors

Tsai MT,Lin DJ,Huang S,Lin HT,Chang WH

doi

10.1007/s00264-011-1259-x

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

199-205

issue

1

eissn

0341-2695

issn

1432-5195

journal_volume

36

pub_type

杂志文章
  • Two-stage procedure in anterior cruciate ligament revision surgery: a five-year follow-up prospective study.

    abstract:PURPOSE:The purpose of this study was to show that this two-stage procedure for ACL (anterior cruciate ligament) revision surgery could be straight-forward and provide satisfactory clinical and functional outcomes. MATERIALS:This is a five-year prospective analysis of clinical and functional data on 30 patients (19 me...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-013-1886-5

    authors: Franceschi F,Papalia R,Del Buono A,Zampogna B,Diaz Balzani L,Maffulli N,Denaro V

    更新日期:2013-07-01 00:00:00

  • The microbiology of the infected knee arthroplasty.

    abstract::We describe the results of a retrospective analysis of patients with microbiologically proven infection, who underwent revision TKA between 1994 and 2008. Of the 121 patients included in the study, 61 (50%) were male and 60 (50%) were female. The mean age was 71 (range 42-88) years. The most common organisms identifie...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-009-0797-y

    authors: Nickinson RS,Board TN,Gambhir AK,Porter ML,Kay PR

    更新日期:2010-04-01 00:00:00

  • The duration of denervation and regeneration in nerve grafting. Quantitative histological assessment in the rat.

    abstract::The common peroneal nerves of 15 male rats were severed and the rats were divided into four groups: immediate grafting (I group); grafting delayed one month (IM group); grafting delayed for three months (3M group) and 5 healthy rats were used as controls (control group). All the rats were sacrificed 5 months after gra...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/BF00266515

    authors: Miyamoto Y,Sugita T,Higaki T,Ikuta Y,Tsuge K

    更新日期:1985-01-01 00:00:00

  • Urinary catheterisation and deep wound infection after hip fracture surgery.

    abstract::We have studied the incidence of deep wound infection after hip fracture surgery and its relationship to urinary catheterisation. Infection after hemiarthroplasty occurred in 16/949 cases and 10/1,143 sliding hip screw fixations. No cases of deep sepsis were seen for the 203 patients treated with an intramedullary nai...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-006-0227-3

    authors: Cumming D,Parker MJ

    更新日期:2007-08-01 00:00:00

  • Tuberculous osteomyelitis. A review of 125 cases.

    abstract::One hundred and twenty-five cases of tuberculous osteomyelitis have been observed by the authors since 1968. The site of the lesion and the clinical and radiological findings have been analysed. The importance of accurate diagnosis by bacteriological and histological investigation is emphasized. Pitfalls in the differ...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/BF00266209

    authors: Martini M,Adjrad A,Boudjemaa A

    更新日期:1986-01-01 00:00:00

  • Induced membrane technique with sequential internal fixation: use of a reinforced spacer for reconstruction of infected bone defects.

    abstract:PURPOSE:To evaluate a novel sequential internal fixation strategy using a reinforced spacer for infected bone defect reconstruction by the induced membrane technique (IMT). METHODS:A retrospective case study was performed among patients treated for infected bone defects by applying this strategy. Following radical deb...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-020-04735-2

    authors: Mathieu L,Tossou-Odjo L,de l'Escalopier N,Demoures T,Baus A,Brachet M,Masquelet AC

    更新日期:2020-09-01 00:00:00

  • Time and cost savings in arthroscopic subacromial decompression: the use of bipolar versus monopolar radiofrequency.

    abstract::There is currently an increased interest in the use of electro surgery in arthroscopy. Since the introduction of the bipolar arthroscopic radiofrequency (RF) wand, it has started to replace the classic Bovie monopolar probe on the assumption that the new technology provides multifunctional devices, combining both tiss...

    journal_title:International orthopaedics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00264-008-0541-z

    authors: Diab MA,Fernandez GN,Elsorafy K

    更新日期:2009-02-01 00:00:00

  • Asymptomatic urinary tract colonisation predisposes to superficial wound infection in elective orthopaedic surgery.

    abstract::There is no evidence surrounding the benefits, effects or clinical outcomes treating asymptomatic urinary tract colonisation. A series of 558 patients undergoing elective admission for orthopaedic surgery were recruited prior to surgery and were screened for urinary tract infection (UTI). Patients had their urine dips...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-008-0573-4

    authors: Ollivere BJ,Ellahee N,Logan K,Miller-Jones JC,Allen PW

    更新日期:2009-06-01 00:00:00

  • Single-bundle versus double-bundle anterior cruciate ligament reconstruction: an up-to-date meta-analysis.

    abstract:PURPOSE:The aim of this meta-analysis was to compare the results of arthroscopic single-bundle and double-bundle anterior cruciate ligament (ACL) reconstruction. METHODS:We systematically searched electronic databases to identify randomised controlled trials (RCTs) in which arthroscopic single-bundle was compared with...

    journal_title:International orthopaedics

    pub_type: 杂志文章,meta分析,评审

    doi:10.1007/s00264-012-1651-1

    authors: Li X,Xu CP,Song JQ,Jiang N,Yu B

    更新日期:2013-02-01 00:00:00

  • Cementless total hip arthroplasty in advanced tuberculosis of the hip.

    abstract:PURPOSE:The use of total hip arthroplasty (THA) to treat advanced tuberculous arthritis, particularly during the active phase, is challenging. The aim of this study was to evaluate the efficacy of cementless THA for advanced hip joint tuberculosis. METHODS:This study reviewed 32 patients (mean age at surgery, 49.4 yea...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-015-2997-y

    authors: Zeng M,Hu Y,Leng Y,Xie J,Wang L,Li M,Zhu J

    更新日期:2015-11-01 00:00:00

  • Effect of antibiotic loading on the shear strength at the stem-cement interface (Shear strength of antibiotic loaded cement).

    abstract::The purpose of this study was to investigate the effects of addition of antibiotics into cement powder on the shear properties of the cement-metal interface. The approach involved adding 800 mg of teicoplanin to 40 g bone cement powder in the t-800 group, 1,600 mg teicoplanin in the t-1,600 group, and no antibiotic in...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-007-0347-4

    authors: Kilicoglu O,Koyuncu LO,Ozden VE,Bozdag E,Sunbuloglu E,Yazicioglu O

    更新日期:2008-08-01 00:00:00

  • The Lyon's sulcus-deepening trochleoplasty in previous unsuccessful patellofemoral surgery.

    abstract:PURPOSE:Trochleoplasty is a relatively rare operation with few published results and it remains a technically demanding procedure which requires careful patient selection. The ideal candidate for surgery remains to be elucidated, and some authors consider it as a good revision option in cases of previous unsuccessful o...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-012-1746-8

    authors: Dejour D,Byn P,Ntagiopoulos PG

    更新日期:2013-03-01 00:00:00

  • Patellar tendon bearing plaster casts in fractures of the tibia.

    abstract::One hundred fractures of the tibial shaft were treated by early weight-bearing in a below knee patellar tendon bearing plaster cast. The rate of union, and the range of knee and ankle motion were assessed. Ninety-five per cent had good or excellent results. This method of treatment is particularly valuable in the thir...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/BF00268506

    authors: Ahmad S,Mam MK,Sethi TS

    更新日期:1989-01-01 00:00:00

  • Viability of loose body fragments in osteochondritis dissecans of the knee. A series of cases.

    abstract::The purpose of this study was to determine if the cartilage from loose osteochondral fragments remains viable. Five patients with OCD of the knee who had undergone surgical treatment (arthroscopic reduction and internal fixation of the loose body) were included. The average age of patients was 13 years (range 10-14 ye...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-010-0951-6

    authors: Pascual-Garrido C,Tanoira I,Muscolo DL,Ayerza MA,Makino A

    更新日期:2010-08-01 00:00:00

  • In vitro release of vancomycin from vancomycin-loaded blood coated demineralised bone.

    abstract::In vitro and in vivo studies have demonstrated the possibility that cancellous bone could be used as a carrier of antibiotics for local delivery. However, the release of antibiotics from the loaded cancellous bone is too rapid and uncertain. We hypothesised that demineralisation of cancellous bone would increase the a...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-002-0384-y

    authors: Rhyu KH,Jung MH,Yoo JJ,Lee MC,Seong SC,Kim HJ

    更新日期:2003-01-01 00:00:00

  • Anterior lumbar vertebral translation following translaminar screw fixation. A report of five cases.

    abstract::Translaminar screw fixation is a good procedure for posterolateral spinal fusion of one or two motion segments. Anterior translation of the upper fused vertebra occurred in 5 of our patients following this procedure. A further fusion with pedicle screw fixation was needed in 2 of them. This complication has been previ...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s002640050213

    authors: Plötz GM,Benini A

    更新日期:1998-01-01 00:00:00

  • The impact of standard combined anteversion definitions on gait and clinical outcome within one year after total hip arthroplasty.

    abstract:PURPOSE:Different target areas within the concept of combined cup and stem anteversion have been published for total hip arthroplasty (THA). We asked whether component positioning according to eight standard combined anteversion rules is associated with (1) more physiological gait patterns, (2) higher improvement of ga...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-015-2777-8

    authors: Weber M,Weber T,Woerner M,Craiovan B,Worlicek M,Winkler S,Grifka J,Renkawitz T

    更新日期:2015-12-01 00:00:00

  • Patterns of single segment non-physeal extremity fractures in children.

    abstract::Five hundred and fifteen children with single segment non-physeal extremity fractures were retrospectively reviewed. The male to female ratio was 2.7:1. The average age of study group was 9.9+/-4.7 years; 273 fractures (53%) were on the left, 242 (47%) were on the right. A fall was the main cause of the injury in most...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s002640050388

    authors: Aktas S,Saridogan K,Moralar U,Ture M

    更新日期:1999-01-01 00:00:00

  • The use of porous corundum ceramics in spinal surgery.

    abstract::Between 1987 and 1991 the spine was stabilised in 205 patients using corundum ceramic implants. The radiological results were assessed with a follow up of from 6 to 24 months and were graded as good in 81%. Poor results were not due to the implanted material, but were usually caused by failure to obtain full correctio...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/BF00180170

    authors: Kiwerski JE,Ogonowski A,Bieniek J,Krasuski M

    更新日期:1994-02-01 00:00:00

  • Kirschner wire versus Herbert screw fixation for the treatment of unstable scaphoid waist fracture nonunion using corticocancellous iliac bone graft: randomized clinical trial.

    abstract:PURPOSE:The study compared the impact of the Kirschner wires versus Herbert screw fixation on the rate of union, time to union, correction of deformity, and clinical outcome in adults with unstable scaphoid waist fracture nonunions without avascular necrosis. METHODS:We prospectively randomized 122 patients to undergo...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-020-04730-7

    authors: Hegazy G,Alshal E,Abdelaal M,Abdelaziz M,Moawad M,Saqr YM,El-Sebaey I,Abdelazeem M,El-Barody M

    更新日期:2020-11-01 00:00:00

  • The role of MRSA screening in joint-replacement surgery.

    abstract::The aim of this prospective study was to determine the effect of screening for methicillin-resistant Staphylococcus aureus (MRSA), in patients undergoing total hip and knee replacements, on reducing hospital-acquired infections and the length of hospital stay. We included 395 patients admitted to the elective orthopae...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-005-0649-3

    authors: Sankar B,Hopgood P,Bell KM

    更新日期:2005-06-01 00:00:00

  • Reliability of radiographic landmarks in medial patello-femoral ligament reconstruction in relation to the anatomical femoral torsion.

    abstract:PURPOSE:Anatomically correct graft positioning at the femoral insertion site is a key factor in surgical reconstruction of the medial patello-femoral ligament (MPFL). Basically there are two techniques to define this point in fluoroscopy during surgery. The role of the anatomical femoral torsion on the accuracy and rep...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-014-2523-7

    authors: Kaipel M,Schützenberger S,Farr S,Gergely I,Vlcek A,Kainberger F,Boszotta H,Pretterklieber M

    更新日期:2015-03-01 00:00:00

  • Does stem design influence component positioning in total hip arthroplasty using a minimal invasive posterolateral approach?

    abstract:PURPOSE:Limited data exist for the reconstructive potential of short bone-preserving stems in THA using a minimal invasive posterolateral approach. Our study aim was to assess the effect of stem design on the reconstruction of hip offset and leg length in MIS posterolateral THA. METHODS:This retrospective consecutive ...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-014-2299-9

    authors: Waldstein W,Merle C,Schmidt-Braekling T,Boettner F

    更新日期:2014-07-01 00:00:00

  • Outcomes of lumbar facet syndrome treated with oral diclofenac or methylprednisolone facet injection: a randomized trial.

    abstract:PURPOSE:This prospective randomized trial is to determine the effectiveness of treating lumbar facet syndrome with oral diclofenac, methylprednisolone facet joint injection or both. METHODS:We enrolled lumbar facet syndrome patients treated at Srinagarind Hospital. Enrolled patients were randomly assigned to receive 1...

    journal_title:International orthopaedics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00264-016-3154-y

    authors: Sae-Jung S,Jirarattanaphochai K

    更新日期:2016-06-01 00:00:00

  • Load cell analysis following silastic arthroplasty of the hallux.

    abstract::The first metatarsophalangeal joint was replaced by a flexible silastic hinge prosthesis in 33 joints of 25 patients. Most patients were pleased with the operation but one third had some discomfort under the second and third metatarsal heads. We used a computer linked load cell matrix to analyse the forces under these...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/BF00266950

    authors: Beverly MC,Horan FT,Hutton WC

    更新日期:1985-01-01 00:00:00

  • Contoured plating for proximal ulna nonunion: an improved technique.

    abstract::Twenty-one patients having proximal ulna nonunion were treated with contoured plating. The most proximal hole received an intramedullary 6.5-mm cancellous lag screw that extends fixation of the nonunion site. Mean age of the patients was 41.7 years. Average follow-up period was 22 months. Union occurred in 19 cases af...

    journal_title:International orthopaedics

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00264-009-0809-y

    authors: Khalil A,Elsayed M,Seleem O

    更新日期:2010-03-01 00:00:00

  • Lengthening osteotomy at the intertrochanteric level with simultaneous correction of angular deformities.

    abstract::Fixed multiplane deformities around the hip representing a combination of rotational, angular, and leg-length discrepancies are disabling for the patient and pose a challenge for the orthopaedic surgeon. We describe a joint-preserving, one-stage procedure to address these complex problems using an intertrochanteric os...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s002640100282

    authors: Marti RK,ten Holder EJ,Kloen P

    更新日期:2001-01-01 00:00:00

  • Spinal tuberculosis in HIV positive and negative patients: immunological response and clinical outcome.

    abstract::We measured cytokine secretion patterns in peripheral blood and granulation tissue by flow cytometry in 16 human immunodeficiency virus (HIV) positive and 26 HIV negative patients with spinal tuberculosis. Anti-retroviral therapy was not prescribed. There were no significant differences in the postoperative morbidity ...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s002640000125

    authors: Govender S,Annamalai K,Kumar KP,Govender UG

    更新日期:2000-01-01 00:00:00

  • Avascular necrosis of the femoral head: inter- and intraobserver variations of Ficat and ARCO classifications.

    abstract::Diagnosis of avascular necrosis (AVN) of the femoral head depends on the combination of clinical symptoms and evaluation of radiographs and/or magnetic resonance imaging (MRI). To evaluate the evolution of AVN, the Ficat and the Association Research Circulation Osseous (ARCO) classification are commonly used to assess...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-007-0320-2

    authors: Schmitt-Sody M,Kirchhoff C,Mayer W,Goebel M,Jansson V

    更新日期:2008-06-01 00:00:00

  • Does patient-specific instrumentation increase the risk of notching in the anterior femoral cortex in total knee arthroplasty? A comparative prospective trial.

    abstract:PURPOSE:Patient-specific instrumentation (PSI) was usually applied in total knee arthroplasty (TKA) to acquire a favourable alignment. We hypothesized that using PSI had a potential risk of notching in the anterior femoral cortex, because the femoral component may be placed in an overextension position due to the dista...

    journal_title:International orthopaedics

    pub_type: 杂志文章

    doi:10.1007/s00264-020-04779-4

    authors: Ke S,Ran T,He Y,Lv M,Song X,Zhou Y,Xu Y,Wang M

    更新日期:2020-12-01 00:00:00