Abstract:
PURPOSE:The present study was designed to address whether osteoblasts play a synergistic role in promoting mesenchymal stem cell (MSC) osteogenesis in a direct cell-cell contact co-culture model. METHODS:Murine C3H10T1/2 and MC3T3-E1 cell lines were mixed and plated onto 12-well culture plates and co-cultured at various ratios of initial cell densities. To compare the possible improvement on osteogenic differentiation, co-culture cells were served with or without osteogenic supplements in culture medium. RESULTS:Weak osteogenesis was induced in MSCs co-cultured in an untreated medium with different ratios of osteoblasts. An osteoblast-dependent increase in osteogenic gene expression of Runx2, type I collagen, and osteocalcin was observed over time. Moreover, both alkaline phosphatase (ALP) activity and calcium deposition were distinctly enhanced at levels that were proportional to the quantity of osteoblasts in the culture. The increases in mRNA expression and ALP activity were greater in co-cultures treated with osteogenic supplements than in untreated cultures. However, the production of ALP activity followed by a distinct matrix mineralization was lower in osteogenic-treated cultures containing greater numbers of osteoblasts. This suggests that a higher density of osteoblasts may lead to weak osteogenesis of MSCs by direct cell-cell contact co-culture in an untreated environment. Furthermore, additional osteogenic supplements may act synergistically with osteoblasts to accelerate matrix mineralization by reducing the process of osteogenic differentiation in osteogenic treated co-cultures. CONCLUSIONS:The present work may improve the understanding of MSC osteogenesis and may provide benefits for regenerative medicine.
journal_name
Int Orthopjournal_title
International orthopaedicsauthors
Tsai MT,Lin DJ,Huang S,Lin HT,Chang WHdoi
10.1007/s00264-011-1259-xsubject
Has Abstractpub_date
2012-01-01 00:00:00pages
199-205issue
1eissn
0341-2695issn
1432-5195journal_volume
36pub_type
杂志文章abstract:PURPOSE:The purpose of this study was to show that this two-stage procedure for ACL (anterior cruciate ligament) revision surgery could be straight-forward and provide satisfactory clinical and functional outcomes. MATERIALS:This is a five-year prospective analysis of clinical and functional data on 30 patients (19 me...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-013-1886-5
更新日期:2013-07-01 00:00:00
abstract::We describe the results of a retrospective analysis of patients with microbiologically proven infection, who underwent revision TKA between 1994 and 2008. Of the 121 patients included in the study, 61 (50%) were male and 60 (50%) were female. The mean age was 71 (range 42-88) years. The most common organisms identifie...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-009-0797-y
更新日期:2010-04-01 00:00:00
abstract::The common peroneal nerves of 15 male rats were severed and the rats were divided into four groups: immediate grafting (I group); grafting delayed one month (IM group); grafting delayed for three months (3M group) and 5 healthy rats were used as controls (control group). All the rats were sacrificed 5 months after gra...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00266515
更新日期:1985-01-01 00:00:00
abstract::We have studied the incidence of deep wound infection after hip fracture surgery and its relationship to urinary catheterisation. Infection after hemiarthroplasty occurred in 16/949 cases and 10/1,143 sliding hip screw fixations. No cases of deep sepsis were seen for the 203 patients treated with an intramedullary nai...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-006-0227-3
更新日期:2007-08-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00266209
更新日期:1986-01-01 00:00:00
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journal_title:International orthopaedics
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更新日期:2020-09-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00264-008-0541-z
更新日期:2009-02-01 00:00:00
abstract::There is no evidence surrounding the benefits, effects or clinical outcomes treating asymptomatic urinary tract colonisation. A series of 558 patients undergoing elective admission for orthopaedic surgery were recruited prior to surgery and were screened for urinary tract infection (UTI). Patients had their urine dips...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-008-0573-4
更新日期:2009-06-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章,meta分析,评审
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更新日期:2013-02-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-015-2997-y
更新日期:2015-11-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-007-0347-4
更新日期:2008-08-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-012-1746-8
更新日期:2013-03-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00268506
更新日期:1989-01-01 00:00:00
abstract::The purpose of this study was to determine if the cartilage from loose osteochondral fragments remains viable. Five patients with OCD of the knee who had undergone surgical treatment (arthroscopic reduction and internal fixation of the loose body) were included. The average age of patients was 13 years (range 10-14 ye...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-010-0951-6
更新日期:2010-08-01 00:00:00
abstract::In vitro and in vivo studies have demonstrated the possibility that cancellous bone could be used as a carrier of antibiotics for local delivery. However, the release of antibiotics from the loaded cancellous bone is too rapid and uncertain. We hypothesised that demineralisation of cancellous bone would increase the a...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-002-0384-y
更新日期:2003-01-01 00:00:00
abstract::Translaminar screw fixation is a good procedure for posterolateral spinal fusion of one or two motion segments. Anterior translation of the upper fused vertebra occurred in 5 of our patients following this procedure. A further fusion with pedicle screw fixation was needed in 2 of them. This complication has been previ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640050213
更新日期:1998-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-015-2777-8
更新日期:2015-12-01 00:00:00
abstract::Five hundred and fifteen children with single segment non-physeal extremity fractures were retrospectively reviewed. The male to female ratio was 2.7:1. The average age of study group was 9.9+/-4.7 years; 273 fractures (53%) were on the left, 242 (47%) were on the right. A fall was the main cause of the injury in most...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640050388
更新日期:1999-01-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00180170
更新日期:1994-02-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04730-7
更新日期:2020-11-01 00:00:00
abstract::The aim of this prospective study was to determine the effect of screening for methicillin-resistant Staphylococcus aureus (MRSA), in patients undergoing total hip and knee replacements, on reducing hospital-acquired infections and the length of hospital stay. We included 395 patients admitted to the elective orthopae...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-005-0649-3
更新日期:2005-06-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2523-7
更新日期:2015-03-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-014-2299-9
更新日期:2014-07-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00264-016-3154-y
更新日期:2016-06-01 00:00:00
abstract::The first metatarsophalangeal joint was replaced by a flexible silastic hinge prosthesis in 33 joints of 25 patients. Most patients were pleased with the operation but one third had some discomfort under the second and third metatarsal heads. We used a computer linked load cell matrix to analyse the forces under these...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/BF00266950
更新日期:1985-01-01 00:00:00
abstract::Twenty-one patients having proximal ulna nonunion were treated with contoured plating. The most proximal hole received an intramedullary 6.5-mm cancellous lag screw that extends fixation of the nonunion site. Mean age of the patients was 41.7 years. Average follow-up period was 22 months. Union occurred in 19 cases af...
journal_title:International orthopaedics
pub_type: 临床试验,杂志文章
doi:10.1007/s00264-009-0809-y
更新日期:2010-03-01 00:00:00
abstract::Fixed multiplane deformities around the hip representing a combination of rotational, angular, and leg-length discrepancies are disabling for the patient and pose a challenge for the orthopaedic surgeon. We describe a joint-preserving, one-stage procedure to address these complex problems using an intertrochanteric os...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640100282
更新日期:2001-01-01 00:00:00
abstract::We measured cytokine secretion patterns in peripheral blood and granulation tissue by flow cytometry in 16 human immunodeficiency virus (HIV) positive and 26 HIV negative patients with spinal tuberculosis. Anti-retroviral therapy was not prescribed. There were no significant differences in the postoperative morbidity ...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s002640000125
更新日期:2000-01-01 00:00:00
abstract::Diagnosis of avascular necrosis (AVN) of the femoral head depends on the combination of clinical symptoms and evaluation of radiographs and/or magnetic resonance imaging (MRI). To evaluate the evolution of AVN, the Ficat and the Association Research Circulation Osseous (ARCO) classification are commonly used to assess...
journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-007-0320-2
更新日期:2008-06-01 00:00:00
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journal_title:International orthopaedics
pub_type: 杂志文章
doi:10.1007/s00264-020-04779-4
更新日期:2020-12-01 00:00:00