Abstract：
:Victims of nerve agents basically require antidotal treatment. There is need for novel antidotes and for therapeutic procedures that are specifically adapted to these patients. To cope with this challenge, in vitro test systems which are easy to handle and allow for conducting long-term studies would be of great benefit. The present work introduces co-cultures of spinal cord and muscle tissue as ex vivo testing systems meeting these criteria. Cell cultures in which functional neuromuscular synapses formed ex vivo were prepared from embryonic mice. Spontaneous muscle activity was recorded by video microscopy. Muscle contractions involved intact neuromuscular transmission as indicated by the effect of succinylcholine, a muscle relaxant that completely abolished muscle activity. At a concentration of 0.75 μM the nerve agent VX reduced the frequency of spontaneous muscle contractions by about 75%. Subsequent application of obidoxime re-established muscle movements. After 24 h of antidotal treatment, muscle activity approached the level of sham-treated cultures and remained stable over the following week. In summary, co-cultures of spinal cord and muscle tissue are promising tools for evaluating the success of antidotal treatment following organophosphate intoxication over a period of at least seven days.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Drexler B,Seeger T,Grasshoff C,Thiermann H,Antkowiak Bdoi
10.1016/j.toxlet.2011.04.010subject
Has Abstractpub_date
2011-09-25 00:00:00pages
89-93issue
1eissn
0378-4274issn
1879-3169pii
S0378-4274(11)00158-5journal_volume
206pub_type
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doi：10.1016/j.toxlet.2009.03.003
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Toxicology letters
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pub_type： 杂志文章
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journal_title：Toxicology letters
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.toxlet.2005.09.012
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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