Upregulation of collagen type 1 in aged murine dermis after transplantation of dermal multipotent cells.

Abstract:

BACKGROUND:Multipotent cells can be isolated from dermis, and have wound-healing and antioxidant effects on human and murine skin. AIM:To investigate the changes in aged dermis after injection of dermal multipotent cells and adult fibroblasts. METHODS:Dermal multipotent cells were isolated and cultured from 3-day-old BALB/c mice by a regular dermal fibroblast culture system. We used various inducers to confirm the multilineage differentiation of these cells, then the dermal multipotent cells were transplanted into the dermis of aged (12-week-old) BALB/c mice. At 2 and 4 weeks after transplantation, we examined dermal thickness, and determined the amounts of collagen type I present, using PCR and western blotting. RESULT:The dermal multipotent cells exhibited adipogenic and osteogenic phenotypes when cultured in the presence of certain inducers. Dermal thickness and collagen content in the dermis also increased in the mice injected with dermal multipotent cells, which also had increased RNA and protein expression of collagen type I. CONCLUSIONS:Collectively, these results indicate that dermal multipotent cells are more effective than fibroblasts in increasing collagen type I and dermal thickness, which may be of importance in preventing skin ageing.

journal_name

Clin Exp Dermatol

authors

Xue S,Li L

doi

10.1111/j.1365-2230.2011.04071.x

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

775-81

issue

7

eissn

0307-6938

issn

1365-2230

journal_volume

36

pub_type

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