Luminescence imaging using radionuclides: a potential application in molecular imaging.

Abstract:

INTRODUCTION:Nuclear and optical imaging are complementary in many aspects and there would be many advantages when optical imaging probes are prepared using radionuclides rather than classic fluorophores, and when nuclear and optical dual images are obtained using single imaging probe. METHODS:The luminescence intensities of various radionuclides having different decay modes have been assayed using luminescence imaging and in vitro luminometer. Radioiodinated Herceptin was injected into a tumor-bearing mouse, and luminescence and microPET images were obtained. The plant dipped in [(32)P]phosphate solution was scanned in luminescence mode. Radio-TLC plate was also imaged in the same imaging mode. RESULTS:Radionuclides emitting high energy β(+)/β(-) particles showed higher luminescence signals. NIH3T6.7 tumors were detected in both optical and nuclear imaging. The uptake of [(32)P]phosphate in plant was easily followed by luminescence imaging. Radio-TLC plate was visualized and radiochemical purity was quantified using luminescence imaging. CONCLUSION:Many radionuclides with high energetic β(+) or β(-) particles during decay were found to be imaged in luminescence mode due mainly to Cerenkov radiation. 'Cerenkov imaging' provides a new optical imaging platform and an invaluable bridge between optical and nuclear imaging. New optical imaging probes could be easily prepared using well-established radioiodination methods. Cerenkov imaging will have more applications in the research field of plant science and autoradiography.

journal_name

Nucl Med Biol

authors

Park JC,Il An G,Park SI,Oh J,Kim HJ,Su Ha Y,Wang EK,Min Kim K,Kim JY,Lee J,Welch MJ,Yoo J

doi

10.1016/j.nucmedbio.2010.09.003

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

321-9

issue

3

eissn

0969-8051

issn

1872-9614

pii

S0969-8051(10)00442-7

journal_volume

38

pub_type

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