Abstract：
:DEAD-box proteins are found in all domains of life and participate in almost all cellular processes that involve RNA. The presence of DEAD and Helicase_C conserved domains distinguish these proteins. DEAD-box proteins exhibit RNA-dependent ATPase activity in vitro, and several also show RNA helicase activity. In this study, we analyzed the distribution and architecture of DEAD-box proteins among bacterial genomes to gain insight into the evolutionary pathways that have shaped their history. We identified 1,848 unique DEAD-box proteins from 563 bacterial genomes. Bacterial genomes can possess a single copy DEAD-box gene, or up to 12 copies of the gene, such as in Shewanella. The alignment of 1,208 sequences allowed us to perform a robust analysis of the hallmark motifs of DEAD-box proteins and determine the residues that occur at high frequency, some of which were previously overlooked. Bacterial DEAD-box proteins do not generally contain a conserved C-terminal domain, with the exception of some members that possess a DbpA RNA-binding domain (RBD). Phylogenetic analysis showed a separation of DbpA-RBD-containing and DbpA-RBD-lacking sequences and revealed a group of DEAD-box protein genes that expanded mainly in the Proteobacteria. Analysis of DEAD-box proteins from Firmicutes and γ-Proteobacteria, was used to deduce orthologous relationships of the well-studied DEAD-box proteins from Escherichia coli and Bacillus subtilis. These analyses suggest that DbpA-RBD is an ancestral domain that most likely emerged as a specialized domain of the RNA-dependent ATPases. Moreover, these data revealed numerous events of gene family expansion and reduction following speciation.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
López-Ramírez V,Alcaraz LD,Moreno-Hagelsieb G,Olmedo-Álvarez Gdoi
10.1007/s00239-011-9441-8subject
Has Abstractpub_date
2011-04-01 00:00:00pages
413-31issue
4eissn
0022-2844issn
1432-1432journal_volume
72pub_type
杂志文章abstract：:Fox and Woese (1975a) have shown that a model of 5S RNA secondary structure similar to the one originally derived for Chlorella 5S RNA can be generalized with relatively minor variations to all sequenced 5S RNA molecules, i.e. that corresponding base paired regions can be formed at approximately the same positions. We...
journal_title：Journal of molecular evolution
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journal_title：Journal of molecular evolution
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更新日期：2008-08-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 信件,评审
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更新日期：2017-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00239-003-2557-8
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02102477
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF00175824
更新日期：1995-11-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02198844
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更新日期：2018-12-01 00:00:00
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更新日期：1995-06-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002390010151
更新日期：2001-03-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01732374
更新日期：1979-12-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02407357
更新日期：1993-08-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF00174042
更新日期：1995-07-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-003-0019-y
更新日期：2003-01-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-010-9380-9
更新日期：2010-10-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02102651
更新日期：1991-06-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-008-9068-6
更新日期：2008-03-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02352288
更新日期：1996-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00239-006-0016-z
更新日期：2006-08-01 00:00:00
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doi：10.1007/s00239-005-0185-1
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pub_type： 杂志文章
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更新日期：1987-01-01 00:00:00
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更新日期：1996-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-06-01 00:00:00
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更新日期：1999-03-01 00:00:00
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更新日期：1989-10-01 00:00:00
abstract：:Several algae that were previously classified in the phylum Xanthophyta (yellow-green algae) were assigned in 1971 to a new phylum, Eustigmatophyta. It was anticipated that the number of algae reclassified to Eustigmatophyta would increase. However, due to the fact that the morphological characteristics that segregate...
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更新日期：1997-08-01 00:00:00
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更新日期：2009-11-01 00:00:00
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更新日期：2003-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1987-01-01 00:00:00
