Abstract：
BACKGROUND:Animal models to investigate pathophysiology and xenotransplantation require complex techniques and significant animal utilization. The aim of the study was to develop a reliable ex-vivo technique to test xenotransplant interventions. MATERIAL/METHODS:Miniature Swine being utilized for a nonsurvival study acted as donor animals. Kidneys were flushed and rapidly explanted and chilled to 4°C. Kidneys were assigned to be the control (CK) (n=3) and the mate were used as a Xenograft Kidneys (XK) (n=3). Kidneys were perfused on separate Waters RM 3 perfusion devices. Perfusion temperature was 35-37°C and pressure was 100-110/60-70 mmHg at 60 pulses per minute. CKs were reperfused with autologous blood collected at the time of organ procurement. XKs were reperfused using freshly donated whole human blood. Physical characteristics, urine output were recorded. Core needle biopsies were obtained and examined by a blinded pathologist for evidence of antibody mediated rejection. RESULTS:XK kidneys demonstrated homogenous reperfusion which rapidly became patchy at 5-7 minutes. XK kidneys had become complete black and thrombosed by 60-70 minutes. XK biopsies demonstrated peritubular capillaritis. CK kidneys demonstrated homogenous reperfusion and urine production. H&E stain of CKs only demonstrated nonspecific inflammation. CONCLUSIONS:Our ex-vivo porcine xenotransplant model shows early promise as a tool for studying Xeno- associated hyperacute rejection. This technique saves resources and animal utilization.
journal_name
Ann Transplantjournal_title
Annals of transplantationauthors
Guarrera JV,Stone J,Tulipan J,Jhang J,Arrington B,Boykin J,Markowitz G,Ratner LEsubject
Has Abstractpub_date
2011-01-01 00:00:00pages
80-2issue
1eissn
1425-9524issn
2329-0358pii
881644journal_volume
16pub_type
杂志文章abstract：:Advances in molecular biology techniques allowed for introduction of PCR-based methods for HLA typing. In routine HLA typing for organ transplantation serological method is still being used as a standard, although molecular techniques are applied more and more often. The aim of our study was to compare HLA-DR typing u...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:Living liver donation is associated with size-dependent complications. The resectable size and its safety margin should be defined for the safety of donors. The purpose of the present study was to determine if the current partial hepatectomies are done under the safety margin of the resectable size, by measu...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.892490
更新日期：2015-01-26 00:00:00
abstract：:BACKGROUND The number of pregnancies after liver transplantation (LT) is increasing; however, the safety and incidence of complications associated with these pregnancies are still unclear. In this report, we retrospectively assessed the influences and problems associated with post-transplant pregnancy on allografts, r...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.921193
更新日期：2020-06-09 00:00:00
abstract：:Calcium plays a crucial role in physiological process in liver cells however in high concentrations these ions can be pathogenic and lead to cell death. Mechanisms responsible for maintaining calcium ion concentration gradient in physiological conditions include transmembrane transport, storage in intracellular organe...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:This retrospective single-center study was undertaken to assess the occurrence of de novo neoplasms in renal transplant recipients according to the immunosuppressive regimen and time after transplantation. MATERIAL/METHODS:Observation encompassed 1028 patients transplanted between the years 1983-2006 and fo...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：:Experience of Warsaw Pancreas Laboratory is presented. Some improvement in the methods of rat, human and pig pancreases digestion, and in identification of Langerhans islets by means of intravenous injection of I-DTZ was achieved. For immunoisolation of islets, 2 methods were elaborated: capsules containing alginate/p...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to characterize patient antibodies before and after cadaver and/or living-donor kidney transplantation and to correlate these data with the clinical course after transplantation. METHODS:Sera from 69 cadaver, 9 living-related and 2 patients waiting for living-donor kidney transplant...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVES:In the last three decades liver transplantation (LT) has become a standard procedure for terminal liver failure. Anyway the procedure is highly limited by the availability of donor organs. The use of segmental liver grafts from living or cadaver donors are an attractive way to increase the donor pool for LT ...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Transplant renal artery stenosis (TRAS) is a serious vascular complication due to non-specific clinical manifestations, causing serious diagnostic difficulties. Contrast-enhanced ultrasound (CE-US) can complement standard sonographic examination in evaluation of TRAS. MATERIAL AND METHODS:Standard ultrasoun...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.883896
更新日期：2013-04-26 00:00:00
abstract：BACKGROUND:Given the current organ shortage, an accurate assessment of the patient's outcome after orthotopic liver transplantation (OLTX) for fulminant hepatic failure (FHF) is crucial in order to determine the suitability for transplantation. The purpose of this study was to assess whether APACHE II and III scores wo...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:The patient is a 28 year old woman who received a heart transplant in 1992 secondary to hypertrophic cardiomyopathy with unremarkable post-operative course. In the period immediately post transplantation the patient was on a four-drug immunosuppressive regimen which was subsequently changed to standard three-agent the...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:BACKGROUND This study examined the attitude of patients' relatives in South Korea toward organ donation after brain death. MATERIAL AND METHODS A structured questionnaire was used to obtain the information on the attitude toward organ donation for relatives of patients who were admitted to the surgical intensive care ...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.906485
更新日期：2017-10-27 00:00:00
abstract：BACKGROUND:The early phase of MPA exposure has rarely been investigated after solid organ transplantation, especially in heart transplantation patients. We evaluated the association between exposure to mycophenolic acid (MPA), a main metabolite of mycophenolate mofetil (MMF), and clinical events within 3 months after h...
journal_title：Annals of transplantation
pub_type： 临床试验,杂志文章
doi：10.12659/AOT.883812
更新日期：2013-02-05 00:00:00
abstract：:The number of liver grafts obtained from a cadaver can be greatly increased with the application of split liver transplantation. In the last 10 years, pediatric waiting list mortality has been reduced significantly with the use of this form of liver transplantation, which has 2 major forms. In its most commonly used f...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：10.12659/aot.896351
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:Hypomagnesaemia is a frequent complication after liver transplantation (LTx) however; magnesium is not routinely replaced in the perioperative period. MATERIAL/METHODS:The incidence of hypomagnesaemia before and after pediatric LTx was studied in 673 pediatric patients who underwent living-donor liver trans...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.883220
更新日期：2012-04-01 00:00:00
abstract：:BACKGROUND The incidence of surgical complications after kidney transplantation ranges from 10-25%. The purpose of this study was to evaluate if the application of fibrin glue as a preventive agent reduces surgical morbidity after a living-related-donor kidney transplantation. MATERIAL AND METHODS A controlled clinica...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.899821
更新日期：2016-09-20 00:00:00
abstract：UNLABELLED:In spite of progress in biotechnology, isolation of porcine pancreatic islets remains a difficult task with unpredictable results. One reason could be the lack of knowledge as to the expression of extracellular matrix proteins in porcine exocrine and endocrine tissues, particularly in "islet capsules". Such ...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Transplantation of liver has been remarkably effective in the treatment of liver failure and liver-based inherited metabolic diseases and has revolutionized the care of patients with end-stage liver disease. Unfortunately demand for transplantable livers is progressively outpacing the supply of donated cadaver organs,...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：2007-01-01 00:00:00
abstract：:BACKGROUND Numerically, nurses represent the largest healthcare profession, thus setting norms for the quality and safety of direct patient care. Evidence of a global shortage of nurses in all clinical practice settings across different healthcare systems and countries has been documented. The aims of the present stud...
journal_title：Annals of transplantation
pub_type： 杂志文章,多中心研究
doi：10.12659/aot.898305
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:The Czech Bone Marrow Donor Registry (CBMD)--established 9 years ago, operates within the National HLA Centre, a constituent part of the Department of Immunology at the Institute for Clinical and Experimental Medicine. The Czech Cord Blood Register (CSCB) was recently established (in 1996) and started its act...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:BACKGROUND Inflammatory activity of the artery can be assessed by measuring 18F-fluorodeoxyglucose (18F-FDG) uptake with positron emission tomography computed tomography (PET/CT). Improvement in vascular function after renal transplantation has been reported, but no studies have used 18F-FDG PET/CT to examine the chan...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.909212
更新日期：2018-06-15 00:00:00
abstract：:Ano-genital neoplasia is about 20 x more common in renal transplant patients than the general population. Neoplasms in the immunosuppressed are more morbid and mortal because: patients are younger; tumors are more undifferentiated; they have more and larger foci; more sites are involved; neoplasms tend to persist, rec...
journal_title：Annals of transplantation
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:A short review of legal aspects of organ transplantation. The influence of type of consent and the family attitude on organ donation rate in all European countries. A special attention is given to the legal and ethical aspects of living organ and tissue transplantation. ...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Xenotransplantation from pig to humans may be associated with the risk of transmission of porcine endogenous retroviruses (PERVs) that are present in the genome of all pigs and that infect human cells in vitro. However, it remains unclear whether PERVs infect transplant recipients in vivo and, if so, whether...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:BACKGROUND In kidney transplantation, the association of cold ischemia time (CIT), anastomosis time (AT), and delayed graft function (DGF) is particularly detrimental in grafts from marginal donors; however, actual cut-off criteria are still debated. MATERIAL AND METHODS Data from patients >65 years (n=193) and patien...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.896672
更新日期：2016-05-31 00:00:00
abstract：OBJECTIVES:Aim of the study was to assess frequency and risk factors of steroid resistant cellular rejection (SRR) in heart transplant recipients, to determine methods of its treatment, and to evaluate influence of steroid resistant rejection and method of its treatment on short- and long-term results. METHODS:All pts...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:BACKGROUND TruGraf is a blood-based biomarker test that measures differential expression of a collection of genes that have been shown to correlate with surveillance biopsy results. However, in the majority of U.S. transplant centers, surveillance biopsies are not performed. The objectives of this study were to evalua...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.920839
更新日期：2020-04-28 00:00:00
abstract：:BACKGROUND The purpose of this study was to assess the effect of an organ transplantation scandal on the rate of corneal donations and organ donors at the University Hospital Tübingen. MATERIAL AND METHODS Data from all hospital deaths from January 2012 to December 2013 were analyzed retrospectively. Each deceased pat...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.901385
更新日期：2017-07-11 00:00:00
abstract：:BACKGROUND ABO-incompatible (ABO-i) living donor liver transplantation (LDLT) is a feasible alternative for donor liver allograft in emergency situations, especially in Asia, where deceased-donor organs remain scarce. The reported outcomes of ABO-i LDLT after optimal desensitization are comparable to those of ABO-comp...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/AOT.923502
更新日期：2020-09-18 00:00:00
abstract：BACKGROUND:We report our contemporary experiences with renal autotransplantation in patients with complicated renal vascular diseases and/or complex ureteral injuries. Since its first performance, renal autotransplantation has been steadily improved and become a safe and effective procedure. MATERIAL/METHODS:Between 1...
journal_title：Annals of transplantation
pub_type： 杂志文章
doi：10.12659/aot.883690
更新日期：2012-12-31 00:00:00
