Novel hydrophobins from Trichoderma define a new hydrophobin subclass: protein properties, evolution, regulation and processing.

Abstract:

:Hydrophobins are small proteins, characterised by the presence of eight positionally conserved cysteine residues, and are present in all filamentous asco- and basidiomycetes. They are found on the outer surfaces of cell walls of hyphae and conidia, where they mediate interactions between the fungus and the environment. Hydrophobins are conventionally grouped into two classes (class I and II) according to their solubility in solvents, hydropathy profiles and spacing between the conserved cysteines. Here we describe a novel set of hydrophobins from Trichoderma spp. that deviate from this classification in their hydropathy, cysteine spacing and protein surface pattern. Phylogenetic analysis shows that they form separate clades within ascomycete class I hydrophobins. Using T. atroviride as a model, the novel hydrophobins were found to be expressed under conditions of glucose limitation and to be regulated by differential splicing.

journal_name

J Mol Evol

authors

Seidl-Seiboth V,Gruber S,Sezerman U,Schwecke T,Albayrak A,Neuhof T,von Döhren H,Baker SE,Kubicek CP

doi

10.1007/s00239-011-9438-3

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

339-51

issue

4

eissn

0022-2844

issn

1432-1432

journal_volume

72

pub_type

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