Proteomics reveals that redox regulation is disrupted in patients with ethylmalonic encephalopathy.

abstract：:A steady increase in the incidence of osteoarthritis and other rheumatic diseases has been observed in recent decades, including autoimmune conditions such as rheumatoid arthritis, spondyloarthropathies, systemic lupus erythematosus, systemic sclerosis, and Sjögren's syndrome. Rheumatic and autoimmune diseases (RADs) ...

journal_title：Journal of proteome research

authors： Ruiz-Romero C,Lam MPY,Nilsson P,Önnerfjord P,Utz PJ,Van Eyk JE,Venkatraman V,Fert-Bober J,Watt FE,Blanco FJ

更新日期：2019-12-06 00:00:00

abstract：:T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving prep...

journal_title：Journal of proteome research

authors： Carrascal M,Ovelleiro D,Casas V,Gay M,Abian J

更新日期：2008-12-01 00:00:00

abstract：:Protein post-translational modification by ubiquitin-fold modifier 1, UFM1, regulates many biological processes such as response to endoplasmic reticulum stress and regulation of tumor progression. A recent study has indicated that the UFM1-binding and PCI domain-containing protein 1 (UFBP1) is required for the conjug...

journal_title：Journal of proteome research

authors： Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

更新日期：2018-04-06 00:00:00

abstract：:A new fast method for identification and characterization of proteolytic digests of proteins by monolithic liquid chromatography coupled with mass spectrometry has been developed. The advantages of the monolithic columns are a high-pressure stability and low back pressure resulting in higher flow rates for capillary o...

journal_title：Journal of proteome research

authors： Marcus K,Schäfer H,Klaus S,Bunse C,Swart R,Meyer HE

更新日期：2007-02-01 00:00:00

abstract：:Moving from macroscale preparative systems in proteomics to micro- and nanotechnologies offers researchers the ability to deeply profile smaller numbers of cells that are more likely to be encountered in clinical settings. Herein a recently developed microscale proteomic method, microdroplet processing in one pot for ...

journal_title：Journal of proteome research

authors： Weke K,Singh A,Uwugiaren N,Alfaro JA,Wang T,Hupp TR,O'Neill JR,Vojtesek B,Goodlett DR,Williams SM,Zhou M,Kelly RT,Zhu Y,Dapic I

更新日期：2021-01-25 00:00:00

abstract：:Improvements in proteome analysis of erythrocyte membrane proteins by two-dimensional electrophoresis are here reported. In particular, a differential extraction procedure was set up allowing separation of integral membrane proteins from peripheral species. Moreover, the use of dilute Immobiline gels (down to as low a...

journal_title：Journal of proteome research

authors： Bruschi M,Seppi C,Arena S,Musante L,Santucci L,Balduini C,Scaloni A,Lanciotti M,Righetti PG,Candiano G

更新日期：2005-07-01 00:00:00

abstract：:We have obtained the first direct electrochemistry of full-length inducible nitric oxide synthase (iNOS) by entrapping the enzyme in polyethylenimine (PEI) film. The interaction between iNOS and calmodulin (CaM) was then studied, which revealed an enhanced electron-transfer reactivity of the enzyme facilitated by CaM....

journal_title：Journal of proteome research

authors： Xiao H,Zhou H,Chen G,Liu S,Li G

更新日期：2007-04-01 00:00:00

abstract：:There are new challenges for hazard and risk assessment in the chemical industry with regard to REACH legislation in Europe and related activities in the U.S. and Japan, which require the development of novel in vitro models for the molecular characterization of drug- or chemical-related effects replacing conventional...

journal_title：Journal of proteome research

authors： Groebe K,Hayess K,Klemm-Manns M,Schwall G,Wozny W,Steemans M,Peters AK,Sastri C,Jaeckel P,Stegmann W,Zengerling H,Schopf R,Poznanovic S,Stummann TC,Seiler A,Spielmann H,Schrattenholz A

更新日期：2010-11-05 00:00:00

abstract：:Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins)...

journal_title：Journal of proteome research

authors： Siroy A,Cosette P,Seyer D,Lemaître-Guillier C,Vallenet D,Van Dorsselaer A,Boyer-Mariotte S,Jouenne T,Dé E

更新日期：2006-12-01 00:00:00

abstract：:Middle-aged and old left ventricles (LVs) are structurally and functionally very similar. Compared to a young LV, both show increased wall thickness and increased cavity size, with preserved cardiac function. However, when a stressor such as myocardial infarction occurs, striking differences are revealed between young...

journal_title：Journal of proteome research

authors： Dai Q,Escobar GP,Hakala KW,Lambert JM,Weintraub ST,Lindsey ML

更新日期：2008-02-01 00:00:00

abstract：:There are three quantitative phosphoproteomic strategies most commonly used to study receptor tyrosine kinase (RTK) signaling. These strategies quantify changes in: (1) all three forms of phosphosites (phosphoserine, phosphothreonine and phosphotyrosine) following enrichment of phosphopeptides by titanium dioxide or i...

journal_title：Journal of proteome research

authors： Zhang G,Neubert TA

更新日期：2011-12-02 00:00:00

abstract：:Russell and colleagues deserve credit for being the first to use a QconCAT standard to simultaneously quantify both the wild-type and mutant peptides of a protein (i.e., CYP2B6) ( J. Proteome Res. 2013, 12 (12), 5934-5942. DOI: 10.1021/pr400279u). However, the rationale of their study was entirely different from ours ...

journal_title：Journal of proteome research

authors： Shi J,Wang X,Zhu H,Jiang H,Wang D,Nesvizhskii A,Zhu HJ

更新日期：2019-03-01 00:00:00

abstract：:Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...

journal_title：Journal of proteome research

authors： Kanlaya R,Pattanakitsakul SN,Sinchaikul S,Chen ST,Thongboonkerd V

更新日期：2009-05-01 00:00:00

abstract：:Extraction with HClO(4) provides an easy method for efficient enrichment of both histone H1 and HMG proteins from a variety of tissues. Usually, the histone and the HMG proteins are the most abundant components of the extracts, however, other proteins have frequently been observed but only seldom studied in more detai...

journal_title：Journal of proteome research

authors： Zougman A,Wiśniewski JR

更新日期：2006-04-01 00:00:00

abstract：:Clinical trials have been conducted for the neuronal ceroid lipofuscinoses (NCLs), a group of neurodegenerative lysosomal diseases that primarily affect children. Whereas clinical rating systems will evaluate long-term efficacy, biomarkers to measure short-term response to treatment would be extremely valuable. To ide...

journal_title：Journal of proteome research

authors： Sleat DE,Tannous A,Sohar I,Wiseman JA,Zheng H,Qian M,Zhao C,Xin W,Barone R,Sims KB,Moore DF,Lobel P

更新日期：2017-10-06 00:00:00

abstract：:Metabonomics methods have been successfully applied to the drug discovery, toxicology, phytochemistry, and clinical fields. Here, we report a self-developed metabonomics platform which is based on high performance liquid chromatography-mass spectrometry (HPLC-MS) technique and applied to the investigation of acute det...

journal_title：Journal of proteome research

authors： Yang J,Zhao X,Liu X,Wang C,Gao P,Wang J,Li L,Gu J,Yang S,Xu G

更新日期：2006-03-01 00:00:00

abstract：:Transcription regulation translates static genome information to dynamic cell behaviors, making it central to understand how cells interact with and adapt to their environment. However, only a limited number of transcription regulators and their target genes have been identified in the pathogen Mycobacterium tubercul...

journal_title：Journal of proteome research

authors： Zeng J,Cui T,He ZG

更新日期：2012-09-07 00:00:00

abstract：:Proteomic studies were performed to identify the protein species involved in copper (Cu) stress responses in common wheat. Two-week-old wheat seedlings were exposed to 100 μM CuSO4 treatment for 3 days. Growth of shoots and roots was markedly inhibited and lipid peroxidation was greatly increased. Cu was readily absor...

journal_title：Journal of proteome research

authors： Li G,Peng X,Xuan H,Wei L,Yang Y,Guo T,Kang G

更新日期：2013-11-01 00:00:00

abstract：:Fucosylated alpha-fetoprotein (AFP) is a specific tumor marker for hepatocellular carcinomas (HCC). However, the mechanisms underlying the increase in fucosylated AFP in serum of patients with HCC are largely unknown. Recently, we reported that fucosylation is a possible signal for the secretion of glycoproteins into ...

journal_title：Journal of proteome research

authors： Nakagawa T,Takeishi S,Kameyama A,Yagi H,Yoshioka T,Moriwaki K,Masuda T,Matsumoto H,Kato K,Narimatsu H,Taniguchi N,Miyoshi E

更新日期：2010-10-01 00:00:00

abstract：:We report the proteomic characterization and biological activities of the venom of the black-speckled palm pitviper, Bothriechis nigroviridis, a neotropical arboreal pitviper from Costa Rica. In marked contrast to other Bothriechis species investigated, the venom of B. nigroviridis does not possess detectable Zn(2+)-d...

journal_title：Journal of proteome research

authors： Fernández J,Lomonte B,Sanz L,Angulo Y,Gutiérrez JM,Calvete JJ

更新日期：2010-08-06 00:00:00

abstract：:The world is currently facing the COVID-19 pandemic caused by the SARS-CoV-2 virus. The pandemic is causing the death of people around the world, and public and social health measures to slow or prevent the spread of COVID-19 are being implemented with the involvement of all members of society. Research institutions a...

journal_title：Journal of proteome research

authors： Barros RO,Junior FLCC,Pereira WS,Oliveira NMN,Ramos RM

更新日期：2020-11-06 00:00:00

abstract：:Novel biomarker verification assays are urgently required to improve the efficiency of biomarker development. Benefitting from lower development costs, multiple reaction monitoring (MRM) has been used for biomarker verification as an alternative to immunoassay. However, in general MRM analysis, only one sample can be ...

journal_title：Journal of proteome research

authors： Yin HR,Zhang L,Xie LQ,Huang LY,Xu Y,Cai SJ,Yang PY,Lu HJ

更新日期：2013-09-06 00:00:00

abstract：:Direct tissue profiling and imaging mass spectrometry (MS) provides a detailed assessment of the complex protein pattern within a tissue sample. MALDI MS analysis of thin tissue sections results in over of 500 individual protein signals in the mass range of 2 to 70 kDa that directly correlate with protein composition ...

journal_title：Journal of proteome research

authors： Chaurand P,Schwartz SA,Caprioli RM

更新日期：2004-03-01 00:00:00

abstract：:Protein glycosylation was explored by direct nanoESI MS and MS/MS analysis of ZIC-HILIC-enriched proteolytic glycopeptides without further separation or purification. In a previous publication, we demonstrated that a direct MS-based analysis of proteolytic glycopeptides is feasible for a number of proteins (Henning , ...

journal_title：Journal of proteome research

authors： Neue K,Mormann M,Peter-Katalinić J,Pohlentz G

更新日期：2011-05-06 00:00:00

abstract：:Fulminant type 1 diabetes (FT1DM) is a relatively new clinical entity featured by acute destruction of pancreatic beta cells. Clinical consequences of FT1DM could be fatal when timely medications are not provided, suggesting the particular importance of rapid and accurate diagnosis. Here we report a serum metabonomics...

journal_title：Journal of proteome research

authors： Lu J,Zhou J,Bao Y,Chen T,Zhang Y,Zhao A,Qiu Y,Xie G,Wang C,Jia W,Jia W

更新日期：2012-09-07 00:00:00

abstract：:Gene-encoded antimicrobial peptides (AMPs) kill bacteria very efficiently by either lytic mechanisms or inhibition of specific bacterial targets. Proline-rich AMPs (PrAMPs), for example, produced in insects and mammals rely on the second mechanism. They bind to the 70 kDa bacterial heat shock protein DnaK and the 60 k...

journal_title：Journal of proteome research

authors： Volke D,Krizsan A,Berthold N,Knappe D,Hoffmann R

更新日期：2015-08-07 00:00:00

abstract：:A novel linear ion trap (LIT) mass spectrometer with dual matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) ionization sources has been built in the MALDI-LIT-ESI configuration. The design features two independent ion source/ion optical channels connected to opposite ends of a singl...

journal_title：Journal of proteome research

authors： Smith SA,Blake TA,Ifa DR,Cooks RG,Ouyang Z

更新日期：2007-02-01 00:00:00

abstract：:Glioblastoma multiforme (GBM) is the most aggressive malignant primary brain tumor, with a dismal mean survival even with the current standard of care. Although in vitro cell systems can provide mechanistic insight into the regulatory networks governing GBM cell proliferation and migration, clinical samples provide a ...

journal_title：Journal of proteome research

authors： Johnson H,White FM

更新日期：2014-11-07 00:00:00

abstract：:Depletion of DNA replication initiation factors such as CDC7 kinase triggers the origin activation checkpoint in healthy cells and leads to a protective cell cycle arrest at the G1 phase of the mitotic cell division cycle. This protective mechanism is thought to be defective in cancer cells. To investigate how this ch...

journal_title：Journal of proteome research

authors： Mulvey CM,Tudzarova S,Crawford M,Williams GH,Stoeber K,Godovac-Zimmermann J

更新日期：2013-03-01 00:00:00

abstract：:Seasonal nitrogen (N) cycling in temperate deciduous trees involves the accumulation of bark storage proteins (BSPs) in phloem parenchyma and xylem ray cells. BSPs are anabolized using recycled N during autumn leaf senescence and later become a source of N during spring shoot growth as they are catabolized. Little is ...

journal_title：Journal of proteome research

authors： Islam N,Li G,Garrett WM,Lin R,Sriram G,Cooper B,Coleman GD

更新日期：2015-02-06 00:00:00