Abstract:
:In the present work, DNaseI loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) for pulmonary delivery were prepared using emulsion solvent evaporation. The effects of the various formulation and experimental variables on the size and morphological characteristics of the particles as well as on the encapsulation efficiency were investigated. The stability of the encapsulated DNaseI was evaluated and the respirable fraction was determined. Cytotoxicity of the NPs was evaluated on lung epithelial cells. The results showed that by using leucine and dipalmito-phosphatidyl-choline (DPPC), discrete NPs with 76% retained biological activity were prepared. A high respirable fraction (particles below 6 μm) reaching 71.3% was achieved after nebulization of the NP suspension. The results revealed the suitability of the prepared particles for pulmonary delivery and highlighted the role of excipients in the stabilization of DNaseI against the stresses encountered during preparation.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Osman R,Kan PL,Awad G,Mortada N,El-Shamy AE,Alpar Odoi
10.1016/j.ijpharm.2011.02.013subject
Has Abstractpub_date
2011-04-15 00:00:00pages
257-65issue
1-2eissn
0378-5173issn
1873-3476pii
S0378-5173(11)00129-3journal_volume
408pub_type
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