Abstract：
:Polo-like kinase 1 (PLK1) is required for multiple stages of mitosis and has been found to be overexpressed in many human malignancies. Previous studies by our group have revealed PLK1 overexpression as an independent prognostic factor for hepatocellular carcinoma (HCC). However, the underlying mechanisms of the tumorigenetic effects of PLK1 in HCC remain unclear. In this study, we depleted PLK1 in human hepatoma BEL-7402 cells using small interfering RNA. Flow cytometry analysis showed that PLK1 depletion resulted in a threefold increase in the G2/M population, with a resultant decrease in the G(1) population. Importantly, PLK1 depletion reduced the tumorigenicity of BEL-7402 cells in vivo, evidenced by significantly slower tumor growth following subcutaneous innoculation of tumor cells in the flanks of BALB/c nude mice. Furthermore, more apoptotic bodies associated with decreased Survivin protein expression and increased level of active caspase-3 were observed in tumor tissues of the PLK1 depletion group by TUNEL assay, Western blot and immunohistochemical analysis, respectively. Collectively, our findings imply that PLK1 depletion led to G2/M arrest, inhibition of cell proliferation and promotion of apoptosis via downregulation of Survivin expression, suggesting that PLK1 represents a new therapeutic target for HCC.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
He Z,Wu J,Dang H,Lin H,Zheng H,Zhong Ddoi
10.1016/j.canlet.2011.01.007subject
Has Abstractpub_date
2011-04-28 00:00:00pages
92-8issue
2eissn
0304-3835issn
1872-7980pii
S0304-3835(11)00020-6journal_volume
303pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract：:There is an urgent need to elucidate the mechanistic links between obesity and colon cancer. Convincing evidence for the role of Lgr5(+) stem cells in colon tumorigenesis has been established; however, the influence of obesity on stem cell maintenance is unknown. We assessed the effects of high fat (HF) feeding on col...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2015.10.001
更新日期：2015-12-28 00:00:00
abstract：:To shed light on the association of intestinal microflora with the development of colon cancer, we studied the modifying effects of intestinal microflora on the occurrence of 1,2-dimethylhydrazine (DMH)-induced colonic aberrant crypt foci (ACF) in germfree (GF), gnotobiotic (GB) and conventionalized (Cvd) rats. In the...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(97)04698-3
更新日期：1997-02-26 00:00:00
abstract：:Prolonged exposure to elevated levels of estrogen is a risk factor for breast cancer. Though increased cell growth and loss of DNA repair capacity is one of the proposed mechanisms for estrogen-induced cancers, the mechanism through which estrogen induces cell growth and decreases DNA repair capacity is not clear. DNA...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2011.10.022
更新日期：2012-03-01 00:00:00
abstract：:Vascular normalisation, the process that reverses the structural and functional abnormalities seen in tumour-associated vessels, is also accompanied by changes in leucocyte trafficking. Our previous studies have shown the normalisation effects of the agent CD5-2 which acts to stabilise VE-Cadherin leading to increased...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2020.09.026
更新日期：2021-01-01 00:00:00
abstract：:The effect of bile acids on the formation of azoxymethane induced aberrant crypt foci (ACF) was investigated using the fecal stream-excluded colons of colostomized F344 rats. The excluded colon was irrigated with saline or bile acids (1 mg/0.5 ml per day, 5 days/week) for 4 weeks. The mean numbers of ACF per colon in ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(01)00525-0
更新日期：2001-08-28 00:00:00
abstract：:Increasing evidence points to a fundamental role for cancer stem cells (CSC) in the initiation and propagation of many tumors. As such, in the context of glioblastoma multiforme (GBM), the development of treatment strategies specifically targeted towards CSC-like populations may hold significant therapeutic promise. T...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2008.09.034
更新日期：2009-02-18 00:00:00
abstract：:Our previous studies have shown that norepinephrine induces the migratory activity of human PC-3 prostate, SW 480 colon and MDA-MB-468 breast carcinoma cells. In contrast to these results, we show here that human ES-2 ovarian carcinoma cells have a reduced migratory activity after norepinephrine treatment. This inhibi...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2008.09.008
更新日期：2009-02-18 00:00:00
abstract：:The time-dependent promotion activity of 17beta-estradiol (E2) by initiation with N-ethyl-N-nitrosourea (ENU) on induction of mouse uterine endometrial proliferative lesions was examined. Illumination-induced persistent estrous female CD-1 mice were divided into five groups at 9 weeks of age. At 10 weeks of age, mice ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(00)00709-6
更新日期：2001-04-26 00:00:00
abstract：:Despite the common expression of death receptors, many types of cancer including gliomas are resistant to the death receptor ligand (TRAIL). Melatonin antitumoral actions have been extensively described, including oncostatic properties on several tumor types and improvement of chemotherapeutic regimens. Here, we found...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2009.06.016
更新日期：2010-01-28 00:00:00
abstract：:Receptors for all classes of steroid hormones were identified in cytosols of adenocarcinoma of the lung. In contrast, the incidence of receptor in squamous cell carcinoma was limited and small cell carcinomas appeared devoid of receptor. Receptor binding was of high affinity, saturable and localized to the 8, 6-7 and ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(82)90014-3
更新日期：1982-09-01 00:00:00
abstract：:The ability to recognize and respond to universal molecular patterns on invading microorganisms allows our immune system to stay on high alert, sensing danger to our self-integrity. Our own damaged cells and tissues in pathological situations activate similar warning systems as microbes. In this way, the body is able ...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2015.03.031
更新日期：2015-11-28 00:00:00
abstract：:The modifying effects of nonylphenol and genistein on cancer induction were assessed in a multi-organ carcinogenesis model in male F344 rats initially treated with five different carcinogens. In experiment 1 rats received 250 or 25 ppm nonylphenol, or 250 or 25 ppm genistein in their diet for 28 weeks. The total incid...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(02)00684-5
更新日期：2003-03-20 00:00:00
abstract：:Pancreatic adenocarcinoma (PDAC) is associated with poor clinical outcomes and incomplete responses to conventional therapy. Therefore, there is an unmet clinical need to better understand the predisposing factors for pancreatic cancer in hopes of providing early screening to high-risk patients. While select risk fact...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2020.04.022
更新日期：2020-08-10 00:00:00
abstract：:Glioblastoma (GBM) is the most common and aggressive primary brain tumor. Great interest persists in useful therapeutic targets in GBM. Aberrant expression of long non-coding RNAs (lncRNAs) has been functionally associated with many cancers. Here, we elucidated the function and the possible molecular mechanisms of lnc...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2014.12.051
更新日期：2015-04-01 00:00:00
abstract：:Lumican is a small leucine-rich proteoglycan (SLRP) of the extracellular matrix (ECM) with anti-tumor activity. We recently demonstrated that lumican inhibits the migration of melanoma cells and identified beta1 integrin as mediator of this effect [M.F. D'Onofrio, S. Brézillon, T. Baranek, C. Perreau, P.J. Roughley, F...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2009.03.032
更新日期：2009-09-28 00:00:00
abstract：:We induced tolerance to hexadecylphosphocholine (HePC) in the human epidermoid tumor cell line, KB. After 70 weeks of adaptation, the IC50 of HePC in the resistant cells KBr was 32-fold higher than in parental KB cells, and they were 30-fold more resistant to another ether lipid analogue, ET-18-OCH3. The KBr cells als...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(99)00146-9
更新日期：1999-08-03 00:00:00
abstract：:Chemotherapeutic multidrug resistance (MDR) is a significant challenge to overcome in clinic practice. Several mechanisms contribute to MDR, one of which is the augmented drug efflux induced by the upregulation of ABCB1 in cancer cells. Regorafenib, a multikinase inhibitor targeting the RAS/RAF/MEK/ERK pathway, was ap...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2017.03.011
更新日期：2017-06-28 00:00:00
abstract：:The desensitization mechanism of the EGF receptor (EGFR) is important for the regulation of cancer cells. Although the phosphorylation of EGFR at Tyr1045 and Ser1046/1047 (Ser1046/7) reportedly accounts for such desensitization, the precise mechanism still remains unknown. Therefore, the present study investigated the...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2008.11.034
更新日期：2009-05-08 00:00:00
abstract：:Frequent occurrence of chromosome 22q deletions in lung cancer indicates the presence of a tumor suppressor gene on this chromosome arm. The hSNF5/INI1 gene at chromosome 22q11.2 has recently been shown to act as a tumor suppressor in rhabdoid tumors. To investigate whether the hSNF5/INI1 gene located on chromosome 22...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(00)00342-6
更新日期：2000-05-29 00:00:00
abstract：:H. pylori infection causes gastritis, peptic ulcers and gastric cancer. Eradicating H. pylori prevents ulcers, but to what extent this prevents cancer remains unknown, especially if given after intestinal metaplasia has developed. H. pylori infected wild-type (WT) mice do not develop cancer, but mice lacking the tumor...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2015.01.028
更新日期：2015-04-10 00:00:00
abstract：:The role of the gastrointestinal microflora in the nitrate-dependent formation of nitrosoproline was assessed in control and antibiotic-treated rats. Urinary nitrosoproline excretion as an index of in vivo nitrosamine formation was shown to be unaffected by bacterial decontamination of the alimentary tract, and procee...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(84)80050-6
更新日期：1984-12-01 00:00:00
abstract：:Somatic mutations and chromosome instability are hallmarks of genomic aberrations in cancer cells. Aneuploidies represent common manifestations of chromosome instability, which is frequently observed in human embryos and malignant solid tumors. Activation of human endogenous retroviruses (HERV)-derived loci is documen...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2016.08.001
更新日期：2016-10-10 00:00:00
abstract：:Sulofenur treatment (12.5 microM-1 mM) of colon adenocarcinoma cell lines resulted in dose- and time-dependent cell killing. LYc5 cells were viable longer than GC3/c1 cells. Each concentration resulted in elevation of cytosolic calcium [Ca2+]i) for both cell lines. At lower doses, elevation was delayed for LYc5 cells....
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(94)03607-k
更新日期：1995-01-06 00:00:00
abstract：:The tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a lung carcinogen in the rat and believed to be a causative agent for lung cancer in smokers. To exert its carcinogenic potential NNK is metabolically activated by cytochrome P450-catalyzed alpha-hydroxylation. Nicotine and NNK s...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2003.07.004
更新日期：2003-12-08 00:00:00
abstract：:The biological characteristic cell locomotion and invasion, melanin content, metalloproteinases and telomerase activity were studied in a parental mouse melanoma cell line B16 and two descendents B16BL6 and B16F10. The invasive potential of melanoma cells was assayed in a transwell cell culture chamber. Melanin conten...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(00)00656-x
更新日期：2001-01-01 00:00:00
abstract：:Effects of the green tea catechins (GTCs) on the late promotion or progression stage of mammary gland carcinogenesis were examined in female Sprague-Dawley (SD) rats pretreated with 7,12-dimethylbenz(a)anthracene (DMBA). A total of 84 7-week-old rats received a 50 mg/kg body weight intra-gastric dose of DMBA, and star...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(96)04560-0
更新日期：1997-01-30 00:00:00
abstract：:Loss of heterozygosity (LOH) is an important event of tumorigenesis. In this paper, we report the comprehensive LOH analyses with microsatellite markers and their results at chromosome 8p11.1-q13.3 in gastric cancer. The microsatellite markers D8S2323 and D8S2330 exhibited high LOH frequencies, 54.2 and 57.1%, respect...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2004.03.041
更新日期：2004-09-15 00:00:00
abstract：:Starting with an extract derived from the bark of Mundulea sericea Willd. (Leguminosae) that was active in the process of inhibiting 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ornithine decarboxylase activity (ODC) in cultured mouse epidermal ME 308 cells, the isoflavonoid munetone was isolated and identified ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(98)00309-7
更新日期：1999-02-08 00:00:00
abstract：:Multidrug-resistant MCF-7 breast adenocarcinoma cells (originally named MCF-7/AdrR cells and later re-designated NCI/ADR-RES) have served as an important and widely used research tool during the last two decades. However, the real identity of these cells has been in doubt since 1998 and has since been debated. The ori...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2006.01.013
更新日期：2007-01-08 00:00:00
abstract：:Retinoic acid and retinol induced functional and morphological differentiation of human promyelocytic leukemia cells (HL-60) into mature granulocytes, but did not induce functional or morphological differentiation of mouse myeloid leukemia cells (M1). Cellular retinoic acid-binding protein, but not retinol-binding pr...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(81)90079-3
更新日期：1981-06-01 00:00:00
