Abstract:
:Ultraviolet radiation is the major environmental insult to the skin and stimulates the synthesis of melanin in melanocytes, which then distribute it to the neighboring keratinocytes where it confers photo-protection. Skin color results from the paracrine interaction between these two cell types. Recent studies suggest that endocannabinoids are potential mediators in the skin. Here, we investigated whether cannabinoid drugs play a role in melanogenesis and if ultraviolet radiation modifies the cutaneous endocannabinoid system. We used human melanotic melanoma cell line (SK-mel-1) in monoculture or co-culture with human keratinocytes (HaCat). Endocannabinoid levels, cannabinoid receptors expression, and melanin content were evaluated under basal conditions and after ultraviolet-B irradiation (311 nm). We provide evidence that human melanoma cells (SK-mel-1) express CB(1) receptors, and when in co-culture with keratinocytes (HaCat), the selective CB(1) receptor agonist arachidonyl-2-chloroethylamide (ACEA 1 and 10 μM) inhibited (by 33.4 and 37.3%, respectively) basal melanogenesis. In addition, ultraviolet-B-induced melanogenesis in co-cultures was abolished by ACEA 10 μM. Both ACEA inhibitory effects were reversed by AM251 (1 μM), a selective CB(1) antagonist. Furthermore, ultraviolet-B radiation increased endocannabinoids levels only in keratinocytes, whereas CB(1) cannabinoid receptor expression was up-regulated only in melanoma cells. Our results collectively suggest that ultraviolet radiation activates paracrine CB(1)-mediated endocannabinoid signaling to negatively regulate melanin synthesis. The endocannabinoid system in the skin may be a possible target for future therapies in pigmentary disorders.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Magina S,Esteves-Pinto C,Moura E,Serrão MP,Moura D,Petrosino S,Di Marzo V,Vieira-Coelho MAdoi
10.1007/s00403-011-1126-zsubject
Has Abstractpub_date
2011-04-01 00:00:00pages
201-10issue
3eissn
0340-3696issn
1432-069Xjournal_volume
303pub_type
杂志文章abstract::Propylene glycol (propane-1,2-diol) is the only diol widely used in dermatology. Pentane-1,5-diol is mainly used as a plasticizer in cellulose products and adhesives, in dental composites and in brake fluid compositions and as a preservative for grain. However, pentane-1,5-diol is also an effective solvent, water-bind...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0610-8
更新日期:2005-12-01 00:00:00
abstract::Exposure of hairless mice to the light of a germicidal lamp (254 nm) under conditions which are known to induce epidermal DNA synthesis, cell proliferation, and polyamine metabolism produced a marked increase of polyamine excretion in the urine which lasted for many days. The increase was about the same for free and a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417146
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abstract::Recent studies on eosinophilic fasciitis have lead to this investigation of eosinophilia in progressive systemic sclerosis and localized scleroderma. Eosinophilia (eosinophilic count greater than 300/cmm) was found in ten of 63 progressive systemic sclerosis patients (15.8%) and in two of nine localized scleroderma ca...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00406686
更新日期:1981-01-01 00:00:00
abstract::We previously showed that the homeodomain protein HOXC13 is involved in the expression control of the early human hair keratin genes hHa5 and hHa2, which contain specific HOXC13 binding sites in their proximal promoters. Hox specificity is generally thought to be enhanced by the interaction with members of the TALE su...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0623-3
更新日期:2006-02-01 00:00:00
abstract::Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1606-7
更新日期:2016-01-01 00:00:00
abstract::The function of microphages has been studied in two patients with chronic pyoderma vegetans by in vitro determination of phagocytosis as well as chemotaxis. The results showed a striking decrease in the chemotactic activity of the neutrophil granulocytes, a reduced phagocytosis of Candida albicans and Staphylococcus a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:
更新日期:1978-09-28 00:00:00
abstract::Based on reports suggesting aberrant cell-mediated immunity and altered infiltration of immunocompetent cells into the skin in psoriasis, we studied the stimulation of T cells by autologous non-T mononuclear leukocytes (autologous mixed lymphocyte reaction, AMLR) and by epidermal cells isolated from lesional and clini...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417528
更新日期:1986-01-01 00:00:00
abstract::The aim of this study was to investigate the effects of Cervi cornus Colla (CCC) in the reconstruction of skin equivalent (SE). H&E staining showed that SE containing hyaluronic acid (HA) or HA and CCC had a thicker epidermis than the control SE. Immunohistochemical staining showed that p63 was mainly present at the b...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-012-1283-8
更新日期:2013-01-01 00:00:00
abstract::Transcutaneous oxygen pressure (TcPO2) and skin blood flow (as evaluated by laser doppler) (LD) were investigated in 24 systemic sclerosis (SSc) patients in sclerotic skin (dorsal aspect of the hand) and non-sclerotic skin (interscapular region) and in 24 controls matched for sex and age for the same sites. The two pa...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00376614
更新日期:1991-01-01 00:00:00
abstract::A novel point mutation in a patient with vascular type Ehlers-Danlos syndrome is herein reported. The patient experienced pneumothorax and multiple arterial troubles during the past 10 years. The patient's hand skin was atrophic, and a paper-like atrophic scar was seen. The collagen microfibrils from the patient's ski...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-008-0884-8
更新日期:2008-10-01 00:00:00
abstract::Tympanic (Tty), esophageal (Tes), forehead, and hand skin temperatures, as well as the forehead evaporation rate were recorded in six men (four suffering from rosacea and two healthy controls) before, during, and after 1 h of warm bath (38 degrees-39 degrees C). During the last 30 min of the bath, the subject's face w...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00424276
更新日期:1989-01-01 00:00:00
abstract::The immigration of circulating T cells into specific tissues is directed by the interaction between adhesion molecules on lymphocyte subpopulations and their ligands on vascular endothelium. Of these, endothelial leucocyte adhesion molecule (ELAM-1), weakly expressed in normal human skin (NHS), seems to be the counter...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372006
更新日期:1993-01-01 00:00:00
abstract::Linoleic acid is required for the formation and maintenance of the epidermal barrier, but most of the current in vitro keratinocyte culture systems are linoleic acid-deficient. The aim of the present study was to examine the efficiency of linoleic acid uptake in human keratinocyte cultures grown under submerged and ai...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050430
更新日期:1999-07-01 00:00:00
abstract::Heparin-binding EGF-like growth factor (HB-EGF) and amphiregulin (AREG) are the members of EGF family that bind to common EGF receptor (EGFR) in the epidermis. However, the role of these two growth factors in epidermal hyperplasia of psoriasis has not been established. On the other hand, CD4+ T cells are responsible f...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-007-0809-y
更新日期:2008-01-01 00:00:00
abstract::Up to now, bicomponent keratohyalin has only been described for rat epithelium and human intraepidermal sweat ducts and fetal nail organ cells. In normal human interductal epidermis, the keratohyalin appears homogeneous, osmiophilic and stellate in shape. Under pathological conditions, bicomponent keratohyalin has bee...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00493461
更新日期:1990-01-01 00:00:00
abstract::Test results are reported of the effect of salicylic acid on the vasoconstrictive effect of flumethasone pivalate. In specific, the depigmentation of the skin (identical with increase in lightness of the skin) following topical application of flumethasone pivalate was investigated with the aid of an improved spectroph...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417384
更新日期:1981-01-01 00:00:00
abstract::The histological and ultrastructural effects following 3 weeks' topical treatment with two agents (all-trans retinoic acid and a new synthetic retinoid-like substance, CD271) were evaluated on the epidermis and the epithelial wall of the pseudocomedones in rhino mouse skin. The comedolytic effects of these drugs were ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371617
更新日期:1991-01-01 00:00:00
abstract::It has been reported that artificial restoration of barrier function by a water vapor-impermeable membrane after tape stripping induces barrier abrogation in hairless mice, impeding rather than enhancing barrier recovery. To address this issue, we examined the morphologic changes in the epidermis after tape stripping ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050280
更新日期:1998-03-01 00:00:00
abstract::Lichen planus (LP) is a chronic inflammatory, T cell-mediated autoimmune skin disease. Innate immunity could explain the interplay between environmental triggers and the autoimmune cascade leading to disease development. Toll-like receptors (TLRs) are important components of the innate immune system, with no previous ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-012-1267-8
更新日期:2013-03-01 00:00:00
abstract::Electron microscopic aspects in ten cases of normolipidemic cutaneous xanthomatosis have been investigated. Two additional types IV and V hyperlipoproteinaemic xanthomatosis have also been included. Ultrastructural findings in all cases were similar. Abundant histiocytic cells with numerous intracytoplasmic lipid vacu...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00694624
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371642
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journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-013-1381-2
更新日期:2013-09-01 00:00:00
abstract::Estrone (E1)-sulfatase and dehydroepiandrosterone (DHEA)-sulfatase activities were studied in human female epidermis. Skin specimens were obtained by abdominal or plantar biopsies. The apparent Michaelis-Menten constants for E1 and DHEA sulfatases were 35.2 microM and 8.7 microM, respectively. A substrate inhibition w...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404316
更新日期:1985-01-01 00:00:00
abstract::A suction blister device with a sensitive thermometer and with a temperature regulator was constructed. Utilizing this device, it was demonstrated that the cold challenge induces an increase in the skin temperature in cold urticaria patients during the cold challenge and a simultaneous release of histamine into the su...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404624
更新日期:1984-01-01 00:00:00
abstract::A procedure measuring the ornithine decarboxylase (ODC) activity and polyamine formation of intact neonatal mouse epidermal cells in culture has been developed and tested. Basal cells prepared from neonatal mouse epidermis were plated on round 15-mm Lux coverslips, placed in Costar 24 well culture clusters and grown a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509038
更新日期:1982-01-01 00:00:00
abstract::The topical application of a mixture of sugar and povidone--iodine (PI) has been reported to accelerate the healing of cutaneous wounds and ulcers by promoting re-epithelialization and granulation tissue formation as well as having an anti-microbial effect. To clarify the mechanisms accounting for the efficacy of a 70...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-006-0683-z
更新日期:2006-09-01 00:00:00
abstract::Whereas the inflammatory infiltrates of malignant melanoma have been widely investigated, little is known about the infiltrates accompanying benign melanocytic naevi. Using monoclonal antibodies directed against HLA-DR antigens, the CD1 antigen, the transferrin receptor and functionally divergent macrophage subpopulat...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372703
更新日期:1992-01-01 00:00:00
abstract::The expression of IL-8 in psoriasis has been clearly shown with the use of immunocytochemical, RT-PCR and in situ hybridization methods. The presence of its ligand, the IL-8 receptor, has been demonstrated by the RT-PCR technique. We report here a study of the expression of both IL-8 type A and B receptors by immunohi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050218
更新日期:1997-07-01 00:00:00
abstract::Interleukin-17 (IL-17) has been implicated in the pathogenesis of a large number of inflammatory and autoimmune conditions, including skin disorders such as psoriasis. Recently, much data have accumulated on the possible role of IL-17 in the pathogenesis of alopecia areata (AA). In this review, the available informati...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-018-1823-y
更新日期:2018-07-01 00:00:00
abstract::We report the clinical features of 62 consecutive patients with lichen planus observed in 18 months. The largest number of cases occurred between 50 and 70 years of age. Thirty-four patients had lichen planus only. In the remainder, lichen planus was associated with chronic liver diseases (16 cases), immune-related di...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00