Abstract:
:MicroRNAs (miRNAs) are endogenous non-coding RNAs that function as negative regulators of gene expression. Alterations in miRNA expression have been shown to affect tumor growth and response to chemotherapy. In this study, we explored the possible role of miRNAs in cisplatin resistance in esophageal squamous cell carcinoma (ESCC). First we assessed the sensitivity of nine human ESCC cell lines (KYSE series) to cisplatin using an in vitro cell viability assay, and then we compared the miRNA profiles of the cisplatin-sensitive and -resistant cell lines by miRNA microarray analysis. The two groups showed markedly different miRNA expression profiles, and 10 miRNAs were found to be regulated differentially between the two groups. When miR-141, which was the most highly expressed miRNA in the cisplatin-resistant cell lines, was expressed ectopically in the cisplatin-sensitive cell lines, cell viability after cisplatin treatment was increased significantly. Furthermore, we found that miR-141 directly targeted the 3'-untranslated region of YAP1, which is known to have a crucial role in apoptosis induced by DNA-damaging agents, and thus downregulated YAP1 expression. Our study highlights an important regulatory role for miR-141 in the development of cisplatin resistance in ESCC.
journal_name
J Hum Genetjournal_title
Journal of human geneticsauthors
Imanaka Y,Tsuchiya S,Sato F,Shimada Y,Shimizu K,Tsujimoto Gdoi
10.1038/jhg.2011.1subject
Has Abstractpub_date
2011-04-01 00:00:00pages
270-6issue
4eissn
1434-5161issn
1435-232Xpii
jhg20111journal_volume
56pub_type
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journal_title:Journal of human genetics
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doi:10.1038/jhg.2010.99
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journal_title:Journal of human genetics
pub_type: 杂志文章,评审
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journal_title:Journal of human genetics
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journal_title:Journal of human genetics
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journal_title:Journal of human genetics
pub_type: 杂志文章,多中心研究
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abstract::Breast and/or ovarian cancer (BOC) are among the most frequently diagnosed forms of hereditary cancers and leading cause of death in India. This emphasizes on the need for a cost-effective method for early detection of these cancers. We sequenced 141 unrelated patients and families with BOC using the TruSight Cancer p...
journal_title:Journal of human genetics
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