Analysis of LhcSR3, a protein essential for feedback de-excitation in the green alga Chlamydomonas reinhardtii.

Abstract:

:In photosynthetic organisms, feedback dissipation of excess absorbed light energy balances harvesting of light with metabolic energy consumption. This mechanism prevents photodamage caused by reactive oxygen species produced by the reaction of chlorophyll (Chl) triplet states with O₂. Plants have been found to perform the heat dissipation in specific proteins, binding Chls and carotenoids (Cars), that belong to the Lhc family, while triggering of the process is performed by the PsbS subunit, needed for lumenal pH detection. PsbS is not found in algae, suggesting important differences in energy-dependent quenching (qE) machinery. Consistent with this suggestion, a different Lhc-like gene product, called LhcSR3 (formerly known as LI818) has been found to be essential for qE in Chlamydomonas reinhardtii. In this work, we report the production of two recombinant LhcSR isoforms from C. reinhardtii and their biochemical and spectroscopic characterization. We found the following: (i) LhcSR isoforms are Chl a/b- and xanthophyll-binding proteins, contrary to higher plant PsbS; (ii) the LhcSR3 isoform, accumulating in high light, is a strong quencher of Chl excited states, exhibiting a very fast fluorescence decay, with lifetimes below 100 ps, capable of dissipating excitation energy from neighbor antenna proteins; (iii) the LhcSR3 isoform is highly active in the transient formation of Car radical cation, a species proposed to act as a quencher in the heat dissipation process. Remarkably, the radical cation signal is detected at wavelengths corresponding to the Car lutein, rather than to zeaxanthin, implying that the latter, predominant in plants, is not essential; (iv) LhcSR3 is responsive to low pH, the trigger of non-photochemical quenching, since it binds the non-photochemical quenching inhibitor dicyclohexylcarbodiimide, and increases its energy dissipation properties upon acidification. This is the first report of an isolated Lhc protein constitutively active in energy dissipation in its purified form, opening the way to detailed molecular analysis. Owing to its protonatable residues and constitutive excitation energy dissipation, this protein appears to merge both pH-sensing and energy-quenching functions, accomplished respectively by PsbS and monomeric Lhcb proteins in plants.

journal_name

PLoS Biol

journal_title

PLoS biology

authors

Bonente G,Ballottari M,Truong TB,Morosinotto T,Ahn TK,Fleming GR,Niyogi KK,Bassi R

doi

10.1371/journal.pbio.1000577

subject

Has Abstract

pub_date

2011-01-18 00:00:00

pages

e1000577

issue

1

eissn

1544-9173

issn

1545-7885

journal_volume

9

pub_type

杂志文章
  • A signaling protease required for melanization in Drosophila affects resistance and tolerance of infections.

    abstract::Organisms evolve two routes to surviving infections-they can resist pathogen growth (resistance) and they can endure the pathogenesis of infection (tolerance). The sum of these two properties together defines the defensive capabilities of the host. Typically, studies of animal defenses focus on either understanding re...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0060305

    authors: Ayres JS,Schneider DS

    更新日期:2008-12-09 00:00:00

  • Neuroinflammation in the normal-appearing white matter (NAWM) of the multiple sclerosis brain causes abnormalities at the nodes of Ranvier.

    abstract::Changes to the structure of nodes of Ranvier in the normal-appearing white matter (NAWM) of multiple sclerosis (MS) brains are associated with chronic inflammation. We show that the paranodal domains in MS NAWM are longer on average than control, with Kv1.2 channels dislocated into the paranode. These pathological fea...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3001008

    authors: Gallego-Delgado P,James R,Browne E,Meng J,Umashankar S,Tan L,Picon C,Mazarakis ND,Faisal AA,Howell OW,Reynolds R

    更新日期:2020-12-14 00:00:00

  • Stable T-bet(+)GATA-3(+) Th1/Th2 hybrid cells arise in vivo, can develop directly from naive precursors, and limit immunopathologic inflammation.

    abstract::Differentiated T helper (Th) cell lineages are thought to emerge from alternative cell fate decisions. However, recent studies indicated that differentiated Th cells can adopt mixed phenotypes during secondary immunological challenges. Here we show that natural primary immune responses against parasites generate bifun...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001633

    authors: Peine M,Rausch S,Helmstetter C,Fröhlich A,Hegazy AN,Kühl AA,Grevelding CG,Höfer T,Hartmann S,Löhning M

    更新日期:2013-01-01 00:00:00

  • A Src-like inactive conformation in the abl tyrosine kinase domain.

    abstract::The improper activation of the Abl tyrosine kinase results in chronic myeloid leukemia (CML). The recognition of an inactive conformation of Abl, in which a catalytically important Asp-Phe-Gly (DFG) motif is flipped by approximately 180 degrees with respect to the active conformation, underlies the specificity of the ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0040144

    authors: Levinson NM,Kuchment O,Shen K,Young MA,Koldobskiy M,Karplus M,Cole PA,Kuriyan J

    更新日期:2006-05-01 00:00:00

  • Anything you can do, you can do better: neural substrates of incentive-based performance enhancement.

    abstract::Performance-based pay schemes in many organizations share the fundamental assumption that the performance level for a given task will increase as a function of the amount of incentive provided. Consistent with this notion, psychological studies have demonstrated that expectations of reward can improve performance on a...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001272

    authors: Liljeholm M,O'Doherty JP

    更新日期:2012-02-01 00:00:00

  • Harvesting the promising fruits of genomics: applying genome sequencing technologies to crop breeding.

    abstract::Next generation sequencing (NGS) technologies are being used to generate whole genome sequences for a wide range of crop species. When combined with precise phenotyping methods, these technologies provide a powerful and rapid tool for identifying the genetic basis of agriculturally important traits and for predicting ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001883

    authors: Varshney RK,Terauchi R,McCouch SR

    更新日期:2014-06-10 00:00:00

  • Correction: dynamic endothelial cell rearrangements drive developmental vessel regression.

    abstract::[This corrects the article DOI: 10.1371/journal.pbio.1002125.]. ...

    journal_title:PLoS biology

    pub_type: 已发布勘误

    doi:10.1371/journal.pbio.1002163

    authors: Franco CA,Jones ML,Bernabeu MO,Geudens I,Mathivet T,Rosa A,Lopes FM,Lima AP,Ragab A,Collins RT,Phng LK,Coveney PV,Gerhardt H

    更新日期:2015-05-14 00:00:00

  • Competition for hosts modulates vast antigenic diversity to generate persistent strain structure in Plasmodium falciparum.

    abstract::In their competition for hosts, parasites with antigens that are novel to the host immune system will be at a competitive advantage. The resulting frequency-dependent selection can structure parasite populations into strains of limited genetic overlap. For the causative agent of malaria, Plasmodium falciparum, the hig...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000336

    authors: Pilosof S,He Q,Tiedje KE,Ruybal-Pesántez S,Day KP,Pascual M

    更新日期:2019-06-24 00:00:00

  • A bioenergetic basis for membrane divergence in archaea and bacteria.

    abstract::Membrane bioenergetics are universal, yet the phospholipid membranes of archaea and bacteria-the deepest branches in the tree of life-are fundamentally different. This deep divergence in membrane chemistry is reflected in other stark differences between the two domains, including ion pumping and DNA replication. We re...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001926

    authors: Sojo V,Pomiankowski A,Lane N

    更新日期:2014-08-12 00:00:00

  • Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation.

    abstract::To complete cell division with high fidelity, cytokinesis must be coordinated with chromosome segregation. Mammalian Polo-like kinase 1, Plk1, may function as a critical link because it is required for chromosome segregation and establishment of the cleavage plane following anaphase onset. A central spindle-localized ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000110

    authors: Wolfe BA,Takaki T,Petronczki M,Glotzer M

    更新日期:2009-05-05 00:00:00

  • Functional dissection of an innate immune response by a genome-wide RNAi screen.

    abstract::The innate immune system is ancient and highly conserved. It is the first line of defense and the only recognizable immune system in the vast majority of metazoans. Signaling events that convert pathogen detection into a defense response are central to innate immunity. Drosophila has emerged as an invaluable model org...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0020203

    authors: Foley E,O'Farrell PH

    更新日期:2004-08-01 00:00:00

  • Location coding by opponent neural populations in the auditory cortex.

    abstract::Although the auditory cortex plays a necessary role in sound localization, physiological investigations in the cortex reveal inhomogeneous sampling of auditory space that is difficult to reconcile with localization behavior under the assumption of local spatial coding. Most neurons respond maximally to sounds located ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0030078

    authors: Stecker GC,Harrington IA,Middlebrooks JC

    更新日期:2005-03-01 00:00:00

  • A role for myosin VI in the localization of axonal proteins.

    abstract::In neurons polarized trafficking of vesicle-bound membrane proteins gives rise to the distinct molecular composition and functional properties of axons and dendrites. Despite their central role in shaping neuronal form and function, surprisingly little is known about the molecular processes that mediate polarized targ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001021

    authors: Lewis TL Jr,Mao T,Arnold DB

    更新日期:2011-03-01 00:00:00

  • Identification and functional characterization of N-terminally acetylated proteins in Drosophila melanogaster.

    abstract::Protein modifications play a major role for most biological processes in living organisms. Amino-terminal acetylation of proteins is a common modification found throughout the tree of life: the N-terminus of a nascent polypeptide chain becomes co-translationally acetylated, often after the removal of the initiating me...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000236

    authors: Goetze S,Qeli E,Mosimann C,Staes A,Gerrits B,Roschitzki B,Mohanty S,Niederer EM,Laczko E,Timmerman E,Lange V,Hafen E,Aebersold R,Vandekerckhove J,Basler K,Ahrens CH,Gevaert K,Brunner E

    更新日期:2009-11-01 00:00:00

  • Interactive youth science workshops benefit student participants and graduate student mentors.

    abstract::Science communication and outreach are essential for training the next generation of scientists and raising public awareness for science. Providing effective science, technology, engineering, and mathematics (STEM) educational outreach to students in classrooms is challenging because of the need to form partnerships w...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000668

    authors: Kompella P,Gracia B,LeBlanc L,Engelman S,Kulkarni C,Desai N,June V,March S,Pattengale S,Rodriguez-Rivera G,Ryu SW,Strohkendl I,Mandke P,Clark G

    更新日期:2020-03-30 00:00:00

  • Role of a novel PH-kinase domain interface in PKB/Akt regulation: structural mechanism for allosteric inhibition.

    abstract::Protein kinase B (PKB/Akt) belongs to the AGC superfamily of related serine/threonine protein kinases. It is a key regulator downstream of various growth factors and hormones and is involved in malignant transformation and chemo-resistance. Full-length PKB protein has not been crystallised, thus studying the molecular...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000017

    authors: Calleja V,Laguerre M,Parker PJ,Larijani B

    更新日期:2009-01-20 00:00:00

  • ADAMTS13 maintains cerebrovascular integrity to ameliorate Alzheimer-like pathology.

    abstract::Blood-brain barrier (BBB) defects and cerebrovascular dysfunction contribute to amyloid-β (Aβ) brain accumulation and drive Alzheimer disease (AD) pathology. By regulating vascular functions and inflammation in the microvasculature, a disintegrin and metalloprotease with thrombospondin type I motif, member 13 (ADAMTS1...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000313

    authors: Cao Y,Xu H,Zhu Y,Shi MJ,Wei L,Zhang J,Cheng S,Shi Y,Tong H,Kang L,Lu L,Luo H,Yang X,Bai X,Wang R,Ma Y,Wang Y,Wang Z,Zhong K,Zhao BQ,Fan W

    更新日期:2019-06-11 00:00:00

  • The fat controller: adipocyte development.

    abstract::Obesity is a condition characterized by excess adipose tissue that results from positive energy balance and is the most common metabolic disorder in the industrialized world. The obesity epidemic shows no sign of slowing, and it is increasingly a global problem. Serious clinical problems associated with obesity includ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001436

    authors: Stephens JM

    更新日期:2012-01-01 00:00:00

  • Cryptochrome mediates light-dependent magnetosensitivity of Drosophila's circadian clock.

    abstract::Since 1960, magnetic fields have been discussed as Zeitgebers for circadian clocks, but the mechanism by which clocks perceive and process magnetic information has remained unknown. Recently, the radical-pair model involving light-activated photoreceptors as magnetic field sensors has gained considerable support, and ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000086

    authors: Yoshii T,Ahmad M,Helfrich-Förster C

    更新日期:2009-04-07 00:00:00

  • Cell-by-cell dissection of gene expression and chromosomal interactions reveals consequences of nuclear reorganization.

    abstract::The functional consequences of long-range nuclear reorganization were studied in a cell-by-cell analysis of gene expression and long-range chromosomal interactions in the Drosophila eye and eye imaginal disk. Position-effect variegation was used to stochastically perturb gene expression and probe nuclear reorganizatio...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0030067

    authors: Harmon B,Sedat J

    更新日期:2005-03-01 00:00:00

  • The homeostatic dynamics of feeding behaviour identify novel mechanisms of anorectic agents.

    abstract::Better understanding of feeding behaviour will be vital in reducing obesity and metabolic syndrome, but we lack a standard model that captures the complexity of feeding behaviour. We construct an accurate stochastic model of rodent feeding at the bout level in order to perform quantitative behavioural analysis. Analys...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000482

    authors: McGrath TM,Spreckley E,Rodriguez AF,Viscomi C,Alamshah A,Akalestou E,Murphy KG,Jones NS

    更新日期:2019-12-05 00:00:00

  • Inhibitor of the tyrosine phosphatase STEP reverses cognitive deficits in a mouse model of Alzheimer's disease.

    abstract::STEP (STriatal-Enriched protein tyrosine Phosphatase) is a neuron-specific phosphatase that regulates N-methyl-D-aspartate receptor (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking, as well as ERK1/2, p38, Fyn, and Pyk2 activity. STEP is overactive in several neuropsychiatr...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001923

    authors: Xu J,Chatterjee M,Baguley TD,Brouillette J,Kurup P,Ghosh D,Kanyo J,Zhang Y,Seyb K,Ononenyi C,Foscue E,Anderson GM,Gresack J,Cuny GD,Glicksman MA,Greengard P,Lam TT,Tautz L,Nairn AC,Ellman JA,Lombroso PJ

    更新日期:2014-08-05 00:00:00

  • The virtual cell animation collection: tools for teaching molecular and cellular biology.

    abstract::A cell is a minifactory in which structures and molecules are assembled, rearranged, disassembled, packaged, sorted, and transported. Because cellular structures and molecules are invisible to the human eye, students often have difficulty conceptualizing the dynamic nature of cells that function at multiple scales acr...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1002118

    authors: Reindl KM,White AR,Johnson C,Vender B,Slator BM,McClean P

    更新日期:2015-04-09 00:00:00

  • Attenuation of self-generated tactile sensations is predictive, not postdictive.

    abstract::When one finger touches the other, the resulting tactile sensation is perceived as weaker than the same stimulus externally imposed. This attenuation of sensation could result from a predictive process that subtracts the expected sensory consequences of the action, or from a postdictive process that alters the percept...

    journal_title:PLoS biology

    pub_type: 临床试验,杂志文章

    doi:10.1371/journal.pbio.0040028

    authors: Bays PM,Flanagan JR,Wolpert DM

    更新日期:2006-02-01 00:00:00

  • A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.

    abstract::Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Droso...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.0060224

    authors: Dobbelaere J,Josué F,Suijkerbuijk S,Baum B,Tapon N,Raff J

    更新日期:2008-09-16 00:00:00

  • The COVID-19 response illustrates that traditional academic reward structures and metrics do not reflect crucial contributions to modern science.

    abstract::The COVID-19 pandemic has motivated many open and collaborative analytical research projects with real-world impact. However, despite their value, such activities are generally overlooked by traditional academic metrics. Science is ultimately improved by analytical work, whether ensuring reproducible and well-document...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.3000913

    authors: Kucharski AJ,Funk S,Eggo RM

    更新日期:2020-10-16 00:00:00

  • Photoperiodic regulation of flowering time through periodic histone deacetylation of the florigen gene FT.

    abstract::The developmental transition from a vegetative to a reproductive phase (i.e., flowering) is timed by the seasonal cue day length or photoperiod in many plant species. Through the photoperiod pathway, inductive day lengths trigger the production of a systemic flowering signal, florigen, to provoke the floral transition...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001649

    authors: Gu X,Wang Y,He Y

    更新日期:2013-09-01 00:00:00

  • Modulating brain oscillations to drive brain function.

    abstract::Do neuronal oscillations play a causal role in brain function? In a study in this issue of PLOS Biology, Helfrich and colleagues address this long-standing question by attempting to drive brain oscillations using transcranial electrical current stimulation. Remarkably, they were able to manipulate visual perception by...

    journal_title:PLoS biology

    pub_type: 评论,杂志文章

    doi:10.1371/journal.pbio.1002032

    authors: Thut G

    更新日期:2014-12-30 00:00:00

  • Regulation of mammary stem/progenitor cells by PTEN/Akt/beta-catenin signaling.

    abstract::Recent evidence suggests that many malignancies, including breast cancer, are driven by a cellular subcomponent that displays stem cell-like properties. The protein phosphatase and tensin homolog (PTEN) is inactivated in a wide range of human cancers, an alteration that is associated with a poor prognosis. Because PTE...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1000121

    authors: Korkaya H,Paulson A,Charafe-Jauffret E,Ginestier C,Brown M,Dutcher J,Clouthier SG,Wicha MS

    更新日期:2009-06-02 00:00:00

  • Research chimpanzees may get a break.

    abstract::A recent report by the Institute of Medicine leaves few urgent reasons standing for the continued use of chimpanzees in biomedical research. It is high time to think about their retirement, Frans de Waal argues, without neglecting prospects for non-invasive research on behavior, cognition, and genetics. ...

    journal_title:PLoS biology

    pub_type: 杂志文章

    doi:10.1371/journal.pbio.1001291

    authors: de Waal FB

    更新日期:2012-01-01 00:00:00