An additional glucose dehydrogenase from Sulfolobus solfataricus: fine-tuning of sugar degradation?

Abstract:

:Within the SulfoSYS (Sulfolobus Systems Biology) project, the effect of temperature on a metabolic network is investigated at the systems level. Sulfolobus solfataricus utilizes an unusual branched ED (Entner-Doudoroff) pathway for sugar degradation that is promiscuous for glucose and galactose. In the course of metabolic pathway reconstruction, a glucose dehydrogenase isoenzyme (GDH-2, SSO3204) was identified. GDH-2 exhibits high similarity to the previously characterized GDH-1 (SSO3003, 61% amino acid identity), but possesses different enzymatic properties, particularly regarding substrate specificity and catalytic efficiency. In contrast with GDH-1, which exhibits broad substrate specificity for C5 and C6 sugars, GDH-2 is absolutely specific for glucose. The comparison of kinetic parameters suggests that GDH-2 might represent the major player in glucose catabolism via the branched ED pathway, whereas GDH-1 might have a dominant role in galactose degradation via the same pathway as well as in different sugar-degradation pathways.

journal_name

Biochem Soc Trans

authors

Haferkamp P,Kutschki S,Treichel J,Hemeda H,Sewczyk K,Hoffmann D,Zaparty M,Siebers B

doi

10.1042/BST0390077

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

77-81

issue

1

eissn

0300-5127

issn

1470-8752

pii

BST0390077

journal_volume

39

pub_type

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