Identification and characterization of a bacterial glutamic peptidase.

abstract：BACKGROUND:Plant latex is the cytoplasm of highly specialized cells known as laticifers, and is thought to have a critical role in defense against herbivorous insects. Proteins abundantly accumulated in latex might therefore be involved in the defense system. RESULTS:We purified latex abundant protein a and b (LA-a an...

journal_title：BMC biochemistry

authors： Kitajima S,Kamei K,Taketani S,Yamaguchi M,Kawai F,Komatsu A,Inukai Y

更新日期：2010-01-28 00:00:00

abstract：BACKGROUND:The anticancer drug, 6-mercaptopurine (6MP) is subjected to metabolic clearance through xanthine oxidase (XOD) mediated hydroxylation, producing 6-thiouric acid (6TUA), which is excreted in urine. This reduces the effective amount of drug available for therapeutic efficacy. Co-administration of allopurinol, ...

journal_title：BMC biochemistry

authors： Kalra S,Jena G,Tikoo K,Mukhopadhyay AK

更新日期：2007-05-18 00:00:00

abstract：BACKGROUND:The Gram-negative bacterium Haemophilus influenzae is a glutathione auxotroph and acquires the redox-active tripeptide by import. The dedicated glutathione transporter belongs to the ATP-binding cassette (ABC)-transporter superfamily and displays more than 60% overall sequence identity with the well-studied ...

journal_title：BMC biochemistry

authors： Vergauwen B,Van der Meeren R,Dansercoer A,Savvides SN

更新日期：2011-11-16 00:00:00

abstract：BACKGROUND:The expression of the drug-metabolizing enzyme cytochrome P450 3A4 (CYP3A4) is regulated by the pregnane x receptor (PXR), which is modulated by numerous signaling pathways, including the cyclin-dependent kinase (Cdk) pathway. Flavonoids, commonly consumed by humans as dietary constituents, have been shown t...

journal_title：BMC biochemistry

authors： Dong H,Lin W,Wu J,Chen T

更新日期：2010-06-16 00:00:00

abstract：BACKGROUND:Electroporation is a common method to introduce foreign molecules into cells, but its molecular basis is poorly understood. Here I investigate the mechanism of pore formation by direct molecular dynamics simulations of phospholipid bilayers of a size of 256 and of more than 2000 lipids as well as simulations...

journal_title：BMC biochemistry

authors： Tieleman DP

更新日期：2004-07-19 00:00:00

abstract：BACKGROUND:The 5'-triphosphorylated, 2'-5'-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading viral and cellular RNA leading to inhibition of viral replication. The 2-5A system is tightly controll...

journal_title：BMC biochemistry

authors： Poulsen JB,Kjær KH,Justesen J,Martensen PM

更新日期：2015-06-26 00:00:00

abstract：BACKGROUND:The α-isoform of the Type 1A Phosphoinositide 3-kinases (PI3Kα) has protein kinase activity as well as phosphoinositide lipid kinase activity. The best described substrate for its protein kinase activity is its regulatory subunit, p85α, which becomes phosphorylated on Serine 608. Phosphorylation of Serine 60...

journal_title：BMC biochemistry

authors： Layton MJ,Saad M,Church NL,Pearson RB,Mitchell CA,Phillips WA

更新日期：2012-12-27 00:00:00

abstract：BACKGROUND:The inorganic (Pi) phosphate transporter (PiT) family comprises known and putative Na(+)- or H(+)-dependent Pi-transporting proteins with representatives from all kingdoms. The mammalian members are placed in the outer cell membranes and suggested to supply cells with Pi to maintain house-keeping functions. ...

journal_title：BMC biochemistry

authors： Bøttger P,Pedersen L

更新日期：2011-05-17 00:00:00

abstract：BACKGROUND:Peptide-membrane interactions play a key role in the binding, partitioning and folding of membrane proteins, the activity of antimicrobial and fusion peptides, and a number of other processes. To gain a better understanding of the thermodynamics of such interactions, White and Wimley created an interfacial h...

journal_title：BMC biochemistry

authors： Aliste MP,Tieleman DP

更新日期：2005-12-20 00:00:00

abstract：BACKGROUND:The biosynthesis pathway of Pyrroloquinoline quinone, a bacterial redox active cofactor for numerous alcohol and aldose dehydrogenases, is largely unknown, but it is proven that at least six genes in Klebsiella pneumoniae (PqqA-F) are required, all of which are located in the PQQ-operon. RESULTS:New structu...

journal_title：BMC biochemistry

authors： Puehringer S,Metlitzky M,Schwarzenbacher R

更新日期：2008-03-27 00:00:00

abstract：BACKGROUND:Mycobacterium tuberculosis DNA topoisomerase I is an attractive target for discovery of novel TB drugs that act by enhancing the accumulation of the topoisomerase-DNA cleavage product. It shares a common transesterification domain with other type IA DNA topoisomerases. There is, however, no homology between ...

journal_title：BMC biochemistry

authors： Annamalai T,Dani N,Cheng B,Tse-Dinh YC

更新日期：2009-06-11 00:00:00

abstract：:A functional ubiquitin proteasome system is essential for all eukaryotic cells and therefore any alteration to its components has potential pathological consequences. Though the exact underlying mechanism is unclear, an age-related decrease in proteasome activity weakens cellular capacity to remove oxidatively modifie...

journal_title：BMC biochemistry

authors： Dahlmann B

更新日期：2007-11-22 00:00:00

abstract：BACKGROUND:The Dact family of scaffold proteins was discovered by virtue of binding to Dvl proteins central to Wnt and Planar Cell Polarity (PCP) signaling. Subsequently Dact proteins have been linked to a growing list of potential partners implicated in β-catenin-dependent and β-catenin-independent forms of Wnt and ot...

journal_title：BMC biochemistry

authors： Kivimäe S,Yang XY,Cheyette BN

更新日期：2011-06-30 00:00:00

abstract：BACKGROUND:The serotonin type 3 receptor (5-HT3R) is a member of a superfamily of ligand gated ion channels. All members of this family share a large degree of sequence homology and presumably significant structural similarity. A large number of studies have explored the structure-function relationships of members of t...

journal_title：BMC biochemistry

authors： Venkataraman P,Venkatachalan SP,Joshi PR,Muthalagi M,Schulte MK

更新日期：2002-06-13 00:00:00

abstract：BACKGROUND:In mammals, L-threonine is an indispensable amino acid. The conversion of L-threonine to glycine occurs through a two-step biochemical pathway involving the enzymes L-threonine 3-dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. The L-threonine 3-dehydrogenase enzyme has been purified and character...

journal_title：BMC biochemistry

authors： Edgar AJ

更新日期：2002-06-25 00:00:00

abstract：BACKGROUND:The pyridoxal-5'-phosphate (PLP)-dependent or vitamin B6-dependent enzymes that catalyze manifold reactions in the metabolism of amino acids belong to no fewer than four evolutionarily independent protein families. The multiple evolutionary origin and the essential mechanistic role of PLP in these enzymes ar...

journal_title：BMC biochemistry

authors： Vacca RA,Giannattasio S,Capitani G,Marra E,Christen P

更新日期：2008-06-19 00:00:00

abstract：BACKGROUND:Trichomonas vaginalis, a flagellated protozoan, is the agent responsible for trichomoniasis, the most common nonviral sexually transmitted infection worldwide. A reported 200 million cases are documented each year with far more cases going unreported. However, T. vaginalis is disproportionality under studied...

journal_title：BMC biochemistry

authors： Dirkx M,Boyer MP,Pradhan P,Brittingham A,Wilson WA

更新日期：2014-06-28 00:00:00

abstract：BACKGROUNDS:Streptococcus pneumoniae expresses three distinct sialidases, NanA, NanB, and NanC, that are believed to be key virulence factors and thus, potential important drug targets. We previously reported that the three enzymes release different products from sialosides, but could share a common catalytic mechanism...

journal_title：BMC biochemistry

authors： Hayre JK,Xu G,Borgianni L,Taylor GL,Andrew PW,Docquier JD,Oggioni MR

更新日期：2012-10-02 00:00:00

abstract：BACKGROUND:ATP-binding cassette transporter A1 (ABCA1) mediates the lipidation of exchangeable apolipoproteins, the rate-limiting step in the formation of high density lipoproteins (HDL). We previously demonstrated that HDL oxidized ex vivo by peroxidase-generated tyrosyl radical (tyrosylated HDL, tyrHDL) increases the...

journal_title：BMC biochemistry

authors： Hossain MA,Ngeth S,Chan T,Oda MN,Francis GA

更新日期：2012-01-16 00:00:00

abstract：BACKGROUND:General iron-sulfur cluster biosynthesis proceeds through assembly of a transient cluster on IscU followed by its transfer to a recipient apo-protein. The efficiency of the second step is increased by the presence of HscA and HscB, but the reason behind this is poorly understood. To shed light on the functio...

journal_title：BMC biochemistry

authors： Füzéry AK,Oh JJ,Ta DT,Vickery LE,Markley JL

更新日期：2011-01-26 00:00:00

abstract：BACKGROUND:Profilin is a small cytoskeletal protein which interacts with actin, proline-rich proteins and phosphatidylinositol 4,5-bisphosphate (PI(4,5)-P2). Crystallography, NMR and mutagenesis of vertebrate profilins have revealed the amino acid residues that are responsible for the interactions with actin and poly(L...

journal_title：BMC biochemistry

authors： Lambrechts A,Jonckheere V,Dewitte D,Vandekerckhove J,Ampe C

更新日期：2002-05-28 00:00:00

abstract：BACKGROUND:The phosphoethanolamine methyltransferase, PfPMT, of the human malaria parasite Plasmodium falciparum, a member of a newly identified family of phosphoethanolamine methyltransferases (PMT) found solely in some protozoa, nematodes, frogs, and plants, is involved in the synthesis of the major membrane phosphol...

journal_title：BMC biochemistry

authors： Bobenchik AM,Choi JY,Mishra A,Rujan IN,Hao B,Voelker DR,Hoch JC,Mamoun CB

更新日期：2010-01-19 00:00:00

abstract：BACKGROUND:Acetaldehyde, the first metabolite of ethanol, can generate covalent modifications of proteins and cellular constituents. However, functional consequences of such modification remain poorly defined. In the present study, we examined acetaldehyde reaction with human carbonic anhydrase (CA) isozyme II, which h...

journal_title：BMC biochemistry

authors： Bootorabi F,Jänis J,Valjakka J,Isoniemi S,Vainiotalo P,Vullo D,Supuran CT,Waheed A,Sly WS,Niemelä O,Parkkila S

更新日期：2008-11-27 00:00:00

abstract：BACKGROUND:The re-replication inhibitor Geminin binds to several transcription factors including homeodomain proteins, and to members of the polycomb and the SWI/SNF complexes. RESULTS:Here we describe the TATA-binding protein-like factor-interacting protein (TIPT) isoform 2, as a strong binding partner of Geminin. TI...

journal_title：BMC biochemistry

authors： Pitulescu ME,Teichmann M,Luo L,Kessel M

更新日期：2009-06-10 00:00:00

abstract：BACKGROUND:Nudix hydrolases form a protein family whose function is to hydrolyse intracellular nucleotides and so regulate their levels and eliminate potentially toxic derivatives. The genome of the radioresistant bacterium Deinococcus radiodurans encodes 25 nudix hydrolases, an unexpectedly large number. These may con...

journal_title：BMC biochemistry

authors： Fisher DI,Cartwright JL,Harashima H,Kamiya H,McLennan AG

更新日期：2004-05-17 00:00:00

abstract：BACKGROUND:Aberrant mitochondrial function, including excessive reactive oxygen species (ROS) production, has been implicated in the pathogenesis of human diseases. The use of mitochondrial inhibitors to ascertain the sites in the electron transport chain (ETC) resulting in altered ROS production can be an important to...

journal_title：BMC biochemistry

authors： Gusdon AM,Fernandez-Bueno GA,Wohlgemuth S,Fernandez J,Chen J,Mathews CE

更新日期：2015-09-10 00:00:00

abstract：BACKGROUND:Helical repeat motifs are common among regulatory subunits for type-1 and type-2A protein Ser/Thr phosphatases. Yeast Sit4 is a distinctive type-2A phosphatase that has dedicated regulatory subunits named Sit4-Associated Proteins (SAPS). These subunits are conserved, and three human SAPS-related proteins are...

journal_title：BMC biochemistry

authors： Guergnon J,Derewenda U,Edelson JR,Brautigan DL

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:Leptin is an adipocyte-derived hormone that acts via its hypothalamic receptor (LEPRb) to regulate energy balance. A downstream effect essential for the weight-regulatory action of leptin is the phosphorylation and activation of the latent transcription factor STAT3 by LEPRb-associated Janus kinases (JAKs). ...

journal_title：BMC biochemistry

authors： Knobelspies H,Zeidler J,Hekerman P,Bamberg-Lemper S,Becker W

更新日期：2010-01-08 00:00:00

abstract：BACKGROUND:The two myogenic regulatory factors Myf5 and MyoD are basic helix-loop-helix muscle transcription factors undergoing differential cell cycle dependent proteolysis in proliferating myoblasts. This regulated degradation results in the striking expression of these two factors at distinct phases of the cell cycl...

journal_title：BMC biochemistry

authors： Doucet C,Gutierrez GJ,Lindon C,Lorca T,Lledo G,Pinset C,Coux O

更新日期：2005-12-01 00:00:00

abstract：BACKGROUND:Ixodes scapularis, commonly known as the blacklegged or deer tick, is the main vector of Lyme disease in the United States. Recent progress in transcriptome research has uncovered hundreds of different proteins expressed in the salivary glands of hard ticks, the majority of which have no known function, and ...

journal_title：BMC biochemistry

authors： Pichu S,Yalcin EB,Ribeiro JM,King RS,Mather TN

更新日期：2011-06-27 00:00:00