Abstract:
OBJECTIVE:Our aim was to investigate the expression of cytotoxic T lymphocyte-associated molecule 4 (CTLA-4) in ulcerative colitis (UC) and to evaluate the effect of CTLA-4 gene -1661A/G polymorphism on CTLA-4 expression and transcription. METHODS:A total of 20 UC patients and 22 healthy controls matched by age and sex were enrolled at Zhongnan Hospital of Wuhan University in central China. The CTLA-4 -1661A/G polymorphism was genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. A Western blot analysis was performed to determine the full length CTLA-4 (flCTLA-4) protein expression in the peripheral blood of the UC patients. Serum-soluble CTLA-4 (sCTLA-4) levels were measured by enzyme-linked immunosorbent assay. CTLA-4-1661G mutant promoter transcription function was analyzed by site-directed PCR-based mutagenesis. RESULTS:CTLA-4 protein expression on CD4(+) T cells in UC patients was lower than that in the healthy controls (P < 0.001) while serum sCTLA-4 in the UC patients was significantly higher than that in the healthy controls (P < 0.001). No correlation was found between flCTLA-4 and sCTLA-4 expression levels and the -1661 A/G polymorphism of the CTLA-4 gene. Meanwhile, CTLA-4 -1661 allele A had no significant impact on the promoter activity compared with allele G (P > 0.05). CONCLUSION:CTLA-4 expressions were aberrant in UC patients compared with the healthy controls. CTLA-4 -1661A/G polymorphism had no significant impact on CTLA-4 expression and transcription in the peripheral CD4 T cells of UC patients.
journal_name
J Dig Disjournal_title
Journal of digestive diseasesauthors
Jiang T,Ge LQ,Chen ZT,Li C,Zhou F,Luo Y,Xia Bdoi
10.1111/j.1751-2980.2010.00462.xsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
369-75issue
6eissn
1751-2972issn
1751-2980journal_volume
11pub_type
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journal_title:Journal of digestive diseases
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journal_title:Journal of digestive diseases
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journal_title:Journal of digestive diseases
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pub_type: 杂志文章
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journal_title:Journal of digestive diseases
pub_type: 杂志文章
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journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2008.00326.x
更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章,meta分析,评审
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pub_type: 杂志文章,评审
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abstract::Acute non-variceal upper gastrointestinal bleeding (ANVUGIB) is one of the most common medical emergencies in China and worldwide. In 2009, we published the "Guidelines for the diagnosis and treatment of acute non-variceal upper gastrointestinal bleeding" for the patients in China; however, during the past years numer...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,实务指引
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journal_title:Journal of digestive diseases
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更新日期:2011-06-01 00:00:00
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更新日期:2019-06-01 00:00:00
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更新日期:2013-08-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12792
更新日期:2019-08-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2009.00400.x
更新日期:2009-11-01 00:00:00
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journal_title:Journal of digestive diseases
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of digestive diseases
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更新日期:2016-04-01 00:00:00
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更新日期:2017-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/1751-2980.12007
更新日期:2013-01-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12105
更新日期:2014-01-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12857
更新日期:2020-04-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12449
更新日期:2017-02-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12243
更新日期:2015-05-01 00:00:00
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journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/j.1751-2980.2011.00535.x
更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2012-02-01 00:00:00