STEM mode in the SEM: a practical tool for nanotoxicology.

Abstract:

:The addition of a transmitted electron detector to a scanning electron microscope (SEM) allows the recording of bright and dark field scanning transmission electron microscope (STEM) images and the corresponding in-lens secondary electron images from the same region of a thin sample. These combined imaging techniques have been applied here to the analysis of ultrathin sections of cells exposed in vitro to nanomaterials for toxicology investigation. Electron microscopy in general permits the exact nature of the interaction of nanomaterials and cells to be elucidated, and in addition the use of STEM mode in the SEM enables the easy identification and exclusion of artefacts produced by ultramicrotome sectioning. The imaging and analysis obtained by using the STEM mode in the SEM configuration from three different nanomaterial systems of importance (iron oxide nanoparticles, single-walled carbon nanotubes and cadmium selenide quantum dots) indicate that it is a simple, practical and cost-effective tool for nanotoxicological research.

journal_name

Nanotoxicology

journal_title

Nanotoxicology

authors

Hondow N,Harrington J,Brydson R,Doak SH,Singh N,Manshian B,Brown A

doi

10.3109/17435390.2010.535622

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

215-27

issue

2

eissn

1743-5390

issn

1743-5404

journal_volume

5

pub_type

杂志文章
  • In vitro toxicity of functionalised nanoclays is mainly driven by the presence of organic modifiers.

    abstract::Little information exists on the toxicological hazards associated to organo-modified clays. We evaluated the cytotoxicity of a series of pristine and organo-modified nanoclays in different cell lines. The calculated IC50 values for cell viability ranged from 1.4 to 47 µg/mL for the six organoclays used and were above ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2013.776123

    authors: Janer G,Fernández-Rosas E,Mas del Molino E,González-Gálvez D,Vilar G,López-Iglesias C,Ermini V,Vázquez-Campos S

    更新日期:2014-05-01 00:00:00

  • Antigenotoxic potential of boron nitride nanotubes.

    abstract::Boron and boron nitride nanotubes (BNNTs) are increasingly used in different industrial fields and, potentially, in some biomedical areas. As occurs with other nanomaterials (NMs), to increase our knowledge on their potential health hazards is a priority. Although in vitro approaches are a routine in getting biologica...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2018.1482379

    authors: Demir E,Marcos R

    更新日期:2018-10-01 00:00:00

  • Comparative cytotoxicity induced by bulk and nanoparticulated ZnO in the fish and human hepatoma cell lines PLHC-1 and Hep G2.

    abstract::The increasing presence of ZnO nanoparticles (NPs) in consumer products may be having a dramatic impact in aquatic environments. The evaluation of ZnO NP toxicity represents a great challenge. This study aimed at evaluating the cytotoxic effect of micro- and nanosized ZnO in a fish and a mammalian hepatoma cell line. ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2012.676098

    authors: Fernández-Cruz ML,Lammel T,Connolly M,Conde E,Barrado AI,Derick S,Perez Y,Fernandez M,Furger C,Navas JM

    更新日期:2013-08-01 00:00:00

  • Uptake and elimination kinetics of silver nanoparticles and silver nitrate by Raphidocelis subcapitata: The influence of silver behaviour in solution.

    abstract::Raphidocelis subcapitata is a freshwater algae species that constitutes the basis of many aquatic trophic chains. In this study, R. subcapitata was used as a model species to investigate the kinetics of uptake and elimination of silver nanoparticles (AgNP) in comparison to silver nitrate (AgNO3) with particular focus ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2014.963724

    authors: Ribeiro F,Gallego-Urrea JA,Goodhead RM,Van Gestel CA,Moger J,Soares AM,Loureiro S

    更新日期:2015-01-01 00:00:00

  • Uptake of titanium from TiO₂ nanoparticle exposure in the isolated perfused intestine of rainbow trout: nystatin, vanadate and novel CO₂-sensitive components.

    abstract::Nanoparticle (NP) uptake across the gut is poorly understood. In vitro gut sac preparations and isolated perfused intestines were used to investigate the absorption mechanism(s). Exposure of whole gut sacs to 1 mg/l TiO₂ NPs for 4 h caused total Ti metal concentrations to increase in the intestine, with 80% or more of...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2012.735268

    authors: Al-Jubory AR,Handy RD

    更新日期:2013-12-01 00:00:00

  • Oral subchronic exposure to silver nanoparticles causes renal damage through apoptotic impairment and necrotic cell death.

    abstract::Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials. Following oral exposure, AgNPs can accumulate in various organs including kidneys where they show gender specific accumulation. There is limited information on their effect on renal system following long-term animal exposure especially at the ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2017.1343874

    authors: Tiwari R,Singh RD,Khan H,Gangopadhyay S,Mittal S,Singh V,Arjaria N,Shankar J,Roy SK,Singh D,Srivastava V

    更新日期:2017-06-01 00:00:00

  • Occupational exposure to graphene and silica nanoparticles. Part II: pilot study to identify a panel of sensitive biomarkers of genotoxic, oxidative and inflammatory effects on suitable biological matrices.

    abstract::The available biomonitoring studies on workers producing/handling nanomaterials (NMs) focused on potential effects on respiratory, immune and cardio-vascular system. Aim of this study was to identify a panel of sensitive biomarkers and suitable biological matrices to evaluate particularly genotoxic and oxidative effec...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2020.1850903

    authors: Ursini CL,Fresegna AM,Ciervo A,Maiello R,Del Frate V,Folesani G,Galetti M,Poli D,Buresti G,Di Cristo L,Sabella S,Iavicoli S,Cavallo D

    更新日期:2020-12-29 00:00:00

  • Comparative tissue distributions of cadmium chloride and cadmium-based quantum dot 705 in mice: Safety implications and applications.

    abstract::Cadmium (Cd) is a component in quantum dot 705 (QD705). Whether QD705 behaves similar to Cd in vivo is of great concern. We compared the distributional kinetics of cadmium chloride (CdCl(2)) and QD705 in mice after intravenous injection. QD705 showed a longer plasma and body retention than CdCl(2) and could be detecte...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2010.502260

    authors: Yeh TK,Wu JP,Chang LW,Tsai MH,Chang WH,Tsai HT,Yang CS,Lin P

    更新日期:2011-03-01 00:00:00

  • Evaluation of in vivo cytogenetic toxicity of europium hydroxide nanorods (EHNs) in male and female Swiss albino mice.

    abstract::Our group already demonstrated that europium hydroxide nanorods (EHNs) show none or mild toxicity in C57BL/6 mice even at high dose and exhibited excellent pro-angiogenic activity towards in vitro and in vivo models. In the present study, we evaluated the in vivo cytogenetic toxicity of intraperitoneally administered ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2015.1073398

    authors: Bollu VS,Nethi SK,Dasari RK,Rao SS,Misra S,Patra CR

    更新日期:2016-01-01 00:00:00

  • Size and surface charge significantly influence the toxicity of silica and dendritic nanoparticles.

    abstract::The influence of size, surface charge and surface functionality of poly(amido amine) dendrimers and silica nanoparticles (SNPs) on their toxicity was studied in immunocompetent mice. After systematic characterization of nanoparticles, they were administered to CD-1 (caesarean derived-1) mice to evaluate acute toxicity...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2011.604442

    authors: Greish K,Thiagarajan G,Herd H,Price R,Bauer H,Hubbard D,Burckle A,Sadekar S,Yu T,Anwar A,Ray A,Ghandehari H

    更新日期:2012-11-01 00:00:00

  • A novel approach reveals that zinc oxide nanoparticles are bioavailable and toxic after dietary exposures.

    abstract::If engineered nanomaterials are released into the environment, some are likely to end up associated with the food of animals due to aggregation and sorption processes. However, few studies have considered dietary exposure of nanomaterials. Here we show that zinc (Zn) from isotopically modified (67)ZnO particles is eff...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2010.501914

    authors: Croteau MN,Dybowska AD,Luoma SN,Valsami-Jones E

    更新日期:2011-03-01 00:00:00

  • Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway.

    abstract::The biological fate of nanoparticles (NPs) taken up by organisms from their environment is a crucial issue for assessing ecological hazard. Despite its importance, it has scarcely been addressed due to the technical difficulties of doing so in whole organism in vivo studies. Here, by using transmission electron micros...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2018.1487601

    authors: Noventa S,Hacker C,Correia A,Drago C,Galloway T

    更新日期:2018-10-01 00:00:00

  • Effect of titanium dioxide nanoparticles on DNA methylation in multiple human cell lines.

    abstract::Nanoscale titanium dioxide (TiO2) is manufactured in wide scale, with a range of applications in consumer products. Significant toxicity of TiO2 nanoparticles has, however, been recognized, suggesting considerable risk to human health. To evaluate fully their toxicity, assessment of the epigenetic action of these nano...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2020.1723730

    authors: Pogribna M,Koonce NA,Mathew A,Word B,Patri AK,Lyn-Cook B,Hammons G

    更新日期:2020-05-01 00:00:00

  • Ag nanoparticles: size- and surface-dependent effects on model aquatic organisms and uptake evaluation with NanoSIMS.

    abstract::This study aims to assess the effects of Ag particles synthesised by a chemical (Ag 20, 200 nm) and biological method (Ag 23, 27 nm) in aquatic organisms: the bacterium Vibrio fischeri, the alga Desmodesmus subspicatus and the crustacean Daphnia magna. Ag particles exerted toxic effects in all organisms studied with A...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2012.715312

    authors: Georgantzopoulou A,Balachandran YL,Rosenkranz P,Dusinska M,Lankoff A,Wojewodzka M,Kruszewski M,Guignard C,Audinot JN,Girija S,Hoffmann L,Gutleb AC

    更新日期:2013-11-01 00:00:00

  • Dendritic polyglycerol nanoparticles show charge dependent bio-distribution in early human placental explants and reduce hCG secretion.

    abstract::A thorough understanding of nanoparticle bio-distribution at the feto-maternal interface will be a prerequisite for their diagnostic or therapeutic application in women of childbearing age and for teratologic risk assessment. Therefore, the tissue interaction of biocompatible dendritic polyglycerol nanoparticles (dPG-...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2018.1425496

    authors: Juch H,Nikitina L,Reimann S,Gauster M,Dohr G,Obermayer-Pietsch B,Hoch D,Kornmueller K,Haag R

    更新日期:2018-03-01 00:00:00

  • Copper oxide nanoparticles alter cellular morphology via disturbing the actin cytoskeleton dynamics in Arabidopsis roots.

    abstract::Copper oxide nanoparticles (CuO NPs) have severe nano-toxic effects on organisms. Limited data is available on influence of CuO NPs on plant cells. Here, the molecular mechanisms involved in the toxicity of CuO NPs are studied. Exposure to CuO NPs significantly increased copper content in roots (0.062-0.325 mg/g FW), ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2019.1678693

    authors: Jia H,Chen S,Wang X,Shi C,Liu K,Zhang S,Li J

    更新日期:2020-02-01 00:00:00

  • Evaluation of toxicological and teratogenic effects of carbosilane glucose glycodendrimers in zebrafish embryos and model rodent cell lines.

    abstract::Glycodendrimers (Glyco-DDMs) represent a rapidly growing class of nanoparticles with promising properties for biomedical applications but concerns regarding the impact on human health and environment are still justified. Here we report, for the first time, the comparative study of in vivo developmental toxicity of car...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2018.1475582

    authors: Liegertová M,Wrobel D,Herma R,Müllerová M,Šťastná LČ,Cuřínová P,Strašák T,Malý M,Čermák J,Smejkal J,Štofik M,Maly J

    更新日期:2018-10-01 00:00:00

  • Distinct toxic interactions of TiO2 nanoparticles with four coexisting organochlorine contaminants on algae.

    abstract::Engineered nanoparticles are increasingly discharged into the environment. After discharge, these nanoparticles can interact with co-existing organic contaminants, resulting in a phenomena referred to as 'joint toxicity'. This study evaluated joint toxicities of TiO2 nanoparticles (TiO2NPs) with four different (atrazi...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2017.1398358

    authors: Zhang S,Deng R,Lin D,Wu F

    更新日期:2017-01-01 00:00:00

  • Exposure to Al2O3 nanoparticles facilitates conjugative transfer of antibiotic resistance genes from Escherichia coli to Streptomyces.

    abstract::The spread of antibiotic resistance genes (ARGs) has become a global environmental issue; it has been found that nanoparticles (NPs) can promote the transfer of ARGs between bacteria. However, it remains unclear whether NPs can affect this kind of conjugation in Streptomyces, which mainly conjugate with other bacteria...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2019.1669731

    authors: Liu X,Tang J,Song B,Zhen M,Wang L,Giesy JP

    更新日期:2019-12-01 00:00:00

  • Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells.

    abstract::Accumulating evidence indicates that carbon nanotubes (CNTs) are biopersistent and can cause lung damage. With similar fibrous morphology and mode of exposure to asbestos, a known human carcinogen, growing concern has arisen for elevated risk of CNT-induced lung carcinogenesis; however, relatively little is known abou...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2013.801089

    authors: Wang L,Stueckle TA,Mishra A,Derk R,Meighan T,Castranova V,Rojanasakul Y

    更新日期:2014-08-01 00:00:00

  • Oxidative stress studies of six TiO₂ and two CeO₂ nanomaterials: immuno-spin trapping results with DNA.

    abstract::Six TiO₂ and two CeO₂ nanomaterials with dry sizes ranging from 6-410 nm were tested for their ability to cause DNA centered free radicals in vitro in the concentration range of 10-3,000 ug/ml. All eight of the nanomaterials significantly increased the adduction of the spin trap agent 5,5-dimethyl-1-pyroline N-oxide (...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2010.539711

    authors: Kitchin KT,Prasad RY,Wallace K

    更新日期:2011-12-01 00:00:00

  • Risk assessment of amorphous silicon dioxide nanoparticles in a glass cleaner formulation.

    abstract::Since nanomaterials are a heterogeneous group of substances used in various applications, risk assessment needs to be done on a case-by-case basis. Here the authors assess the risk (hazard and exposure) of a glass cleaner with synthetic amorphous silicon dioxide (SAS) nanoparticles during production and consumer use (...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2012.689881

    authors: Michel K,Scheel J,Karsten S,Stelter N,Wind T

    更新日期:2013-08-01 00:00:00

  • In vitro toxicity of nanoceria: effect of coating and stability in biofluids.

    abstract::Due to the increasing use of nanometric cerium oxide in applications, concerns about the toxicity of these particles have been raised and have resulted in a large number of studies. We report here on the interactions between 7 nm anionically charged cerium oxide particles and living mammalian cells. By a modification ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2013.831501

    authors: Ould-Moussa N,Safi M,Guedeau-Boudeville MA,Montero D,Conjeaud H,Berret JF

    更新日期:2014-11-01 00:00:00

  • Copper nanoparticles induce early fibrotic changes in the liver via TGF-β/Smad signaling and cause immunosuppressive effects in rats.

    abstract::Copper nanoparticles (Cu NPs) have various uses, including as additives in polymers/plastics, lubricants for metallic coating, and biomedical applications. We investigated the role of transforming growth factor (TGF)-β1 signaling in hepatic damage caused by Cu NPs and explored the effects of a 28-day repeated oral adm...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2018.1472313

    authors: Lee IC,Ko JW,Park SH,Shin NR,Shin IS,Moon C,Kim SH,Yun WK,Kim HC,Kim JC

    更新日期:2018-08-01 00:00:00

  • Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring.

    abstract::Effects of maternal pulmonary exposure to carbon black (Printex 90) on gestation, lactation and DNA strand breaks were evaluated. Time-mated C57BL/6BomTac mice were exposed by inhalation to 42 mg/m(3) Printex 90 for 1 h/day on gestation days (GD) 8-18, or by four intratracheal instillations on GD 7, 10, 15 and 18, wit...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2011.587902

    authors: Jackson P,Hougaard KS,Boisen AM,Jacobsen NR,Jensen KA,Møller P,Brunborg G,Gutzkow KB,Andersen O,Loft S,Vogel U,Wallin H

    更新日期:2012-08-01 00:00:00

  • A secretomics analysis reveals major differences in the macrophage responses towards different types of carbon nanotubes.

    abstract::Certain types of carbon nanotubes (CNT) can evoke inflammation, fibrosis and mesothelioma in vivo, raising concerns about their potential health effects. It has been recently postulated that NLRP3 inflammasome activation is important in the CNT-induced toxicity. However, more comprehensive studies of the protein secre...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2014.969346

    authors: Palomäki J,Sund J,Vippola M,Kinaret P,Greco D,Savolainen K,Puustinen A,Alenius H

    更新日期:2015-01-01 00:00:00

  • Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles.

    abstract::Iron nanoparticles (NPs) have been proposed as a tool in very different fields such as environmental remediation and biomedical applications, including food fortification against iron deficiency, even if there is still concern about their safety. Here, we propose Xenopus laevis embryos as a suitable model to investiga...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2019.1685695

    authors: Bonfanti P,Colombo A,Saibene M,Fiandra L,Armenia I,Gamberoni F,Gornati R,Bernardini G,Mantecca P

    更新日期:2020-03-01 00:00:00

  • Occupational exposure limit for silver nanoparticles: considerations on the derivation of a general health-based value.

    abstract::With the increased production and widespread commercial use of silver nanoparticles (AgNPs), human and environmental exposures to silver nanoparticles are inevitably increasing. In particular, persons manufacturing and handling silver nanoparticles and silver nanoparticle containing products are at risk of exposure, p...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2016.1148793

    authors: Weldon BA,M Faustman E,Oberdörster G,Workman T,Griffith WC,Kneuer C,Yu IJ

    更新日期:2016-09-01 00:00:00

  • New insights in the acute toxic/genotoxic effects of CuO nanoparticles in the in vivo Drosophila model.

    abstract::Metal oxide nanoparticles are highly reactive from the biological point of view and, for this reason, it exists important reservations in regard human health impact. We used Drosophila as a promising in vivo model to diagnose the biological effects of copper oxide nanoparticles (CuO-NPs). Due to the potential role of ...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.3109/17435390.2015.1121413

    authors: Alaraby M,Hernández A,Marcos R

    更新日期:2016-08-01 00:00:00

  • Assessment of potential toxicity of foodborne fluorescent nanoparticles from roasted pork.

    abstract::Foodborne nanoparticles (NPs) refer to the nanostructures generated during food processing, rather than the man-made nano-structural additives that are added to improve the property of food. In this research, discovery of fluorescent NPs in roasted pork was reported at different temperature of 180, 230, and 280 °C. Th...

    journal_title:Nanotoxicology

    pub_type: 杂志文章

    doi:10.1080/17435390.2019.1652943

    authors: Zhao X,Shan S,Li J,Cao L,Lv J,Tan M

    更新日期:2019-12-01 00:00:00