Abstract:
:The addition of a transmitted electron detector to a scanning electron microscope (SEM) allows the recording of bright and dark field scanning transmission electron microscope (STEM) images and the corresponding in-lens secondary electron images from the same region of a thin sample. These combined imaging techniques have been applied here to the analysis of ultrathin sections of cells exposed in vitro to nanomaterials for toxicology investigation. Electron microscopy in general permits the exact nature of the interaction of nanomaterials and cells to be elucidated, and in addition the use of STEM mode in the SEM enables the easy identification and exclusion of artefacts produced by ultramicrotome sectioning. The imaging and analysis obtained by using the STEM mode in the SEM configuration from three different nanomaterial systems of importance (iron oxide nanoparticles, single-walled carbon nanotubes and cadmium selenide quantum dots) indicate that it is a simple, practical and cost-effective tool for nanotoxicological research.
journal_name
Nanotoxicologyjournal_title
Nanotoxicologyauthors
Hondow N,Harrington J,Brydson R,Doak SH,Singh N,Manshian B,Brown Adoi
10.3109/17435390.2010.535622subject
Has Abstractpub_date
2011-06-01 00:00:00pages
215-27issue
2eissn
1743-5390issn
1743-5404journal_volume
5pub_type
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