Abstract:
:Grain yield in many cereal crops is largely determined by grain size. Here we report the genetic and molecular characterization of GS3, a major quantitative trait locus for grain size. It functions as a negative regulator of grain size and organ size. The wild-type isoform is composed of four putative domains: a plant-specific organ size regulation (OSR) domain in the N terminus, a transmembrane domain, a tumor necrosis factor receptor/nerve growth factor receptor (TNFR/NGFR) family cysteine-rich domain, and a von Willebrand factor type C (VWFC) in the C terminus. These domains function differentially in grain size regulation. The OSR domain is both necessary and sufficient for functioning as a negative regulator. The wild-type allele corresponds to medium grain. Loss of function of OSR results in long grain. The C-terminal TNFR/NGFR and VWFC domains show an inhibitory effect on the OSR function; loss-of-function mutations of these domains produced very short grain. This study linked the functional domains of the GS3 protein to natural variation of grain size in rice.
journal_name
Proc Natl Acad Sci U S Aauthors
Mao H,Sun S,Yao J,Wang C,Yu S,Xu C,Li X,Zhang Qdoi
10.1073/pnas.1014419107subject
Has Abstractpub_date
2010-11-09 00:00:00pages
19579-84issue
45eissn
0027-8424issn
1091-6490pii
1014419107journal_volume
107pub_type
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