Abstract：
:Myosin VI is a molecular motor that is thought to function both as a transporter and as a cytoskeletal anchor in vivo. Here we use optical tweezers to examine force generation by single molecules of myosin VI under physiological nucleotide concentrations. We find that myosin VI is an efficient transporter at loads of up to ∼2 pN but acts as a cytoskeletal anchor at higher loads. Our data and the resulting model are consistent with an indirect coupling of global structural motions to nucleotide binding and release. The model provides a mechanism by which load may regulate the dual functions of myosin VI in vivo. Our results suggest that myosin VI kinetics are tuned such that the motor maintains a consistent level of mechanical tension within the cell, a property potentially shared by other mechanosensitive proteins.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Chuan P,Spudich JA,Dunn ARdoi
10.1016/j.jmb.2010.10.010subject
Has Abstractpub_date
2011-01-07 00:00:00pages
105-12issue
1eissn
0022-2836issn
1089-8638pii
S0022-2836(10)01107-1journal_volume
405pub_type
杂志文章abstract：:We have identified Adrm1 as a novel component of the regulatory ATPase complex of the 26 S proteasome: Adrm1 was precipitated with an antibody to proteasomes and vice versa. Adrm1 co-migrated with proteasomes on gel-filtration chromatography and non-denaturing polyacrylamide gel electrophoresis. Adrm1 has been describ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
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abstract：:Promiscuous mutant EcoRI endonucleases produce lethal to sublethal effects because they cleave Escherichia coli DNA despite the presence of the EcoRI methylase. Three promiscuous mutant forms, Ala138Thr, Glu192Lys and His114Tyr, have been characterized with respect to their binding affinities and first-order cleavage ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2003-04-11 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1997-10-31 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2010-01-22 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2007-05-11 00:00:00
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更新日期：2002-05-03 00:00:00
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pub_type： 杂志文章
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更新日期：2001-09-07 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/j.jmb.2017.01.005
更新日期：2017-05-05 00:00:00
