Regulation of the androgen receptor by SET9-mediated methylation.

Abstract:

:The androgen receptor (AR) is a member of the nuclear hormone receptor family of transcription factors that plays a critical role in regulating expression of genes involved in prostate development and transformation. Upon hormone binding, the AR associates with numerous co-regulator proteins that regulate the activation status of target genes via flux to the post-translational modification status of histones and the receptor. Here we show that the AR interacts with and is directly methylated by the histone methyltransferase enzyme SET9. Methylation of the AR on lysine 632 is necessary for enhancing transcriptional activity of the receptor by facilitating both inter-domain communication between the N- and C-termini and recruitment to androgen-target genes. We also show that SET9 is pro-proliferative and anti-apoptotic in prostate cancer cells and demonstrates up-regulated nuclear expression in prostate cancer tissue. In all, our date indicate a new mechanism of AR regulation that may be therapeutically exploitable for prostate cancer treatment.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Gaughan L,Stockley J,Wang N,McCracken SR,Treumann A,Armstrong K,Shaheen F,Watt K,McEwan IJ,Wang C,Pestell RG,Robson CN

doi

10.1093/nar/gkq861

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

1266-79

issue

4

eissn

0305-1048

issn

1362-4962

pii

gkq861

journal_volume

39

pub_type

杂志文章
  • A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci.

    abstract::A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing S...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/16.13.6027

    authors: Pyle LE,Finch LR

    更新日期:1988-07-11 00:00:00

  • Extensive RNA editing of U to C in addition to C to U substitution in the rbcL transcripts of hornwort chloroplasts and the origin of RNA editing in green plants.

    abstract::We cloned and sequenced a portion of chloroplast DNA from the hornwort Anthoceros formosae. A nucleotide sequence of 7556 bp contained structures similar to those of ndhK, ndhC, trnV, trnM, atpE, atpB, rbcL, trnR and accD. The arrangement of these was the same as that of other chloroplast DNA. However, two nonsense co...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/24.6.1008

    authors: Yoshinaga K,Iinuma H,Masuzawa T,Uedal K

    更新日期:1996-03-15 00:00:00

  • The transfer RNA of certain Enterobacteriacae contain 2-methylthiozeatin riboside (ms2io6A) an isopentenyl adenosine derivative.

    abstract::Isopentenyl adenosine derivatives are always located adjacent to the 3' end of the anticodon in transfer RNA and have been implicated in certain biological functions. In the enteric bacterium, E. coli, the derivative is ms2i6A whereas in some plant associated bacteria the derivative is the hydroxylated form, ms2io6A. ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/10.18.5663

    authors: Janzer JJ,Raney JP,McLennan BD

    更新日期:1982-09-25 00:00:00

  • A high throughput screening for rarely transcribed differentially expressed genes.

    abstract::A novel method combining elements of suppression subtractive hybridization with high throughput differential screening permits the efficient and rapid cloning of rarely transcribed differentially expressed genes. The experimental strategy virtually excludes the possibility of isolating false positive clones. The poten...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/25.13.2598

    authors: von Stein OD,Thies WG,Hofmann M

    更新日期:1997-07-01 00:00:00

  • Specific transcriptional activation in vitro by the herpes simplex virus protein VP16.

    abstract::The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcrip...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/21.24.5570

    authors: Arnosti DN,Preston CM,Hagmann M,Schaffner W,Hope RG,Laughlan G,Luisi BF

    更新日期:1993-12-11 00:00:00

  • An abasic site in DNA. Solution conformation determined by proton NMR and molecular mechanics calculations.

    abstract::We have determined the three-dimensional structure of a non-selfcomplementary nonanucleotide duplex which contains an abasic (apyrimidinic) site in the centre, i.e. a deoxyribose residue opposite an adenosine. The majority of the base and sugar proton resonances were assigned by NOESY, COSY and 2DQF spectra in D2O and...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/15.19.8003

    authors: Cuniasse P,Sowers LC,Eritja R,Kaplan B,Goodman MF,Cognet JA,LeBret M,Guschlbauer W,Fazakerley GV

    更新日期:1987-10-12 00:00:00

  • Different types of interaction between PCNA and PIP boxes contribute to distinct cellular functions of Y-family DNA polymerases.

    abstract::Translesion DNA synthesis (TLS) by the Y-family DNA polymerases Polη, Polι and Polκ, mediated via interaction with proliferating cell nuclear antigen (PCNA), is a crucial pathway that protects human cells against DNA damage. We report that Polη has three PCNA-interacting protein (PIP) boxes (PIP1, 2, 3) that contribut...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkv712

    authors: Masuda Y,Kanao R,Kaji K,Ohmori H,Hanaoka F,Masutani C

    更新日期:2015-09-18 00:00:00

  • Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication.

    abstract::The synthetic DNA fragment (formula, see text) (corresponding to nucleotides 4299-4314 of the phi X DNA sequence) was cloned into either the AmpR gene or the KmR gene of plasmid pACYC 177. The DNA sequence of the KmR gene around the insertion site was determined by nucleotide sequence analysis of the pACYC 177 FnudII ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/9.14.3335

    authors: Heidekamp F,Baas PD,van Boom JH,Veeneman GH,Zipursky SL,Jansz HS

    更新日期:1981-07-24 00:00:00

  • The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair.

    abstract::P53-binding protein 1 (53BP1) mediates DNA repair pathway choice and promotes checkpoint activation. Chromatin marks induced by DNA double-strand breaks and recognized by 53BP1 enable focal accumulation of this multifunctional repair factor at damaged chromatin. Here, we unveil an additional level of regulation of 53B...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkz138

    authors: Salvador Moreno N,Liu J,Haas KM,Parker LL,Chakraborty C,Kron SJ,Hodges K,Miller LD,Langefeld C,Robinson PJ,Lelièvre SA,Vidi PA

    更新日期:2019-04-08 00:00:00

  • WormNet v3: a network-assisted hypothesis-generating server for Caenorhabditis elegans.

    abstract::High-throughput experimental technologies gradually shift the paradigm of biological research from hypothesis-validation toward hypothesis-generation science. Translating diverse types of large-scale experimental data into testable hypotheses, however, remains a daunting task. We previously demonstrated that heterogen...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gku367

    authors: Cho A,Shin J,Hwang S,Kim C,Shim H,Kim H,Kim H,Lee I

    更新日期:2014-07-01 00:00:00

  • Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2'-deoxyuridine or 5-bromo-2'-deoxycytidine.

    abstract::The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely rela...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkl892

    authors: Zeng Y,Wang Y

    更新日期:2006-01-01 00:00:00

  • Structure of HIV-1 TAR RNA in the absence of ligands reveals a novel conformation of the trinucleotide bulge.

    abstract::Efficient transcription from the human immunodeficiency virus (HIV) promoter depends on binding of the viral regulatory protein Tat to a cis-acting RNA regulatory element, TAR. Tat binds at a trinucleotide bulge located near the apex of the TAR stem-loop structure. An essential feature of Tat-TAR interaction is that t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/24.20.3974

    authors: Aboul-ela F,Karn J,Varani G

    更新日期:1996-10-15 00:00:00

  • Mutant isolation of mouse DNA topoisomerase II alpha in yeast.

    abstract::For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a heterologous mammalian ge...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/22.20.4229

    authors: Adachi N,Ikeda H,Kikuchi A

    更新日期:1994-10-11 00:00:00

  • The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b.

    abstract::ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F(0)F(...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkg326

    authors: Burger G,Lang BF,Braun HP,Marx S

    更新日期:2003-05-01 00:00:00

  • Evolutionary Conserved Motif Finder (ECMFinder) for genome-wide identification of clustered YY1- and CTCF-binding sites.

    abstract::We have developed a new bioinformatics approach called ECMFinder (Evolutionary Conserved Motif Finder). This program searches for a given DNA motif within the entire genome of one species and uses the gene association information of a potential transcription factor-binding site (TFBS) to screen the homologous regions ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkp077

    authors: Kang K,Chung JH,Kim J

    更新日期:2009-04-01 00:00:00

  • PubChem in 2021: new data content and improved web interfaces.

    abstract::PubChem (https://pubchem.ncbi.nlm.nih.gov) is a popular chemical information resource that serves the scientific community as well as the general public, with millions of unique users per month. In the past two years, PubChem made substantial improvements. Data from more than 100 new data sources were added to PubChem...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkaa971

    authors: Kim S,Chen J,Cheng T,Gindulyte A,He J,He S,Li Q,Shoemaker BA,Thiessen PA,Yu B,Zaslavsky L,Zhang J,Bolton EE

    更新日期:2021-01-08 00:00:00

  • Mechanical properties of DNA-like polymers.

    abstract::The molecular structure of the DNA double helix has been known for 60 years, but we remain surprisingly ignorant of the balance of forces that determine its mechanical properties. The DNA double helix is among the stiffest of all biopolymers, but neither theory nor experiment has provided a coherent understanding of t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkt808

    authors: Peters JP,Yelgaonkar SP,Srivatsan SG,Tor Y,James Maher L 3rd

    更新日期:2013-12-01 00:00:00

  • DNA supercoiling and aerobic regulation of transcription from the Klebsiella pneumoniae nifLA promoter.

    abstract::Expression from the K. pneumoniae nifLA promoter is oxygen sensitive and is also inhibited by the DNA gyrase inhibitor coumermycin A1 under anaerobic growth conditions. The activity of this promoter was found to be highly sensitive to changes in DNA topology in vitro. Transcription was completely dependent on negative...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/16.21.9933

    authors: Dixon RA,Henderson NC,Austin S

    更新日期:1988-11-11 00:00:00

  • A kinetochore-based ATM/ATR-independent DNA damage checkpoint maintains genomic integrity in trypanosomes.

    abstract::DNA damage-induced cell cycle checkpoints serve as surveillance mechanisms to maintain genomic stability, and are regulated by ATM/ATR-mediated signaling pathways that are conserved from yeast to humans. Trypanosoma brucei, an early divergent microbial eukaryote, lacks key components of the conventional DNA damage-ind...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkz476

    authors: Zhou Q,Pham KTM,Hu H,Kurasawa Y,Li Z

    更新日期:2019-09-05 00:00:00

  • A catalytic antioxidant metalloporphyrin blocks hydrogen peroxide-induced mitochondrial DNA damage.

    abstract::Reactive oxygen species (ROS) have been implicated as the cause of cumulative damage to DNA, proteins and lipids that can ultimately result in cell death. A common problem when measuring oxidative DNA damage has been the introduction of modifications in the native state of the molecule by many DNA isolation methods. W...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/28.4.968

    authors: Milano J,Day BJ

    更新日期:2000-02-15 00:00:00

  • Genome engineering of isogenic human ES cells to model autism disorders.

    abstract::Isogenic pluripotent stem cells are critical tools for studying human neurological diseases by allowing one to study the effects of a mutation in a fixed genetic background. Of particular interest are the spectrum of autism disorders, some of which are monogenic such as Timothy syndrome (TS); others are multigenic suc...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkv164

    authors: Martinez RA,Stein JL,Krostag AR,Nelson AM,Marken JS,Menon V,May RC,Yao Z,Kaykas A,Geschwind DH,Grimley JS

    更新日期:2015-05-26 00:00:00

  • Evolutionarily divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia.

    abstract::Non-coding RNAs (ncRNAs) have diverse essential biological functions in all organisms, and in eukaryotes, two such classes of ncRNAs are the small nucleolar (sno) and small nuclear (sn) RNAs. In this study, we have identified and characterized a collection of sno and snRNAs in Giardia lamblia, by exploiting our discov...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gks887

    authors: Hudson AJ,Moore AN,Elniski D,Joseph J,Yee J,Russell AG

    更新日期:2012-11-01 00:00:00

  • Identification of cis-acting elements in the SUC2 promoter of Saccharomyces cerevisiae required for activation of transcription.

    abstract::We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces cerevisiae. Analyses were performed in wild-type as well as mig1 and tup1 mutant strains after the promoter mutants were reintroduced into the native SUC2 locus on the left arm of chromosome IX. Mutation of the two GC boxes reveal...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/26.4.1002

    authors: Bu Y,Schmidt MC

    更新日期:1998-02-15 00:00:00

  • Functional characterization of two human MutY homolog (hMYH) missense mutations (R227W and V232F) that lie within the putative hMSH6 binding domain and are associated with hMYH polyposis.

    abstract::The base excision repair DNA glycosylase MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro-8-oxo-guanine (8-oxoG), thereby preventing G:C to T:A mutations. Biallelic germline mutations in the human MYH gene predispose individuals to multiple colorectal ade...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gki209

    authors: Bai H,Jones S,Guan X,Wilson TM,Sampson JR,Cheadle JP,Lu AL

    更新日期:2005-01-26 00:00:00

  • Inter-individual variation of DNA methylation and its implications for large-scale epigenome mapping.

    abstract::Genomic DNA methylation profiles exhibit substantial variation within the human population, with important functional implications for gene regulation. So far little is known about the characteristics and determinants of DNA methylation variation among healthy individuals. We performed bioinformatic analysis of high-r...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkn122

    authors: Bock C,Walter J,Paulsen M,Lengauer T

    更新日期:2008-06-01 00:00:00

  • Secondary structure of splice sites in adenovirus mRNA precursors.

    abstract::In order to investigate the possible role of RNA secondary structure in determining the efficiency and specificity of mRNA splicing, the structures of sequences at three acceptor splice sites in adenovirus were studied. Transcripts spanning intron-exon junctions were synthesized using SP6 RNA polymerase and analyzed u...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/12.22.8437

    authors: Munroe SH

    更新日期:1984-11-26 00:00:00

  • Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.

    abstract::Cadmium (Cd2+) is a known carcinogen that inactivates the DNA mismatch repair (MMR) pathway. In this study, we have tested the effect of Cd2+ exposure on the enzymatic activity of the mismatch binding complex MSH2-MSH6. Our results indicate that Cd2+ is highly inhibitory to the ATP binding and hydrolysis activities of...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gki291

    authors: Banerjee S,Flores-Rozas H

    更新日期:2005-03-03 00:00:00

  • Open TG-GATEs: a large-scale toxicogenomics database.

    abstract::Toxicogenomics focuses on assessing the safety of compounds using gene expression profiles. Gene expression signatures from large toxicogenomics databases are expected to perform better than small databases in identifying biomarkers for the prediction and evaluation of drug safety based on a compound's toxicological m...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gku955

    authors: Igarashi Y,Nakatsu N,Yamashita T,Ono A,Ohno Y,Urushidani T,Yamada H

    更新日期:2015-01-01 00:00:00

  • SDPMOD: an automated comparative modeling server for small disulfide-bonded proteins.

    abstract::Small disulfide-bonded proteins (SDPs) are rich sources for therapeutic drugs. Designing drugs from these proteins requires three-dimensional structural information, which is only available for a subset of these proteins. SDPMOD addresses this deficit in structural information by providing a freely available automated...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/gkh394

    authors: Kong L,Lee BT,Tong JC,Tan TW,Ranganathan S

    更新日期:2004-07-01 00:00:00

  • The effect of codon usage on the oligonucleotide composition of the E. coli genome and identification of over- and underrepresented sequences by Markov chain analysis.

    abstract::As shown in the accompanying paper (5), the oligonucleotide composition of the E. coli genome is highly asymmetric for sequences up to 6 bp in length when ranked from highest to lowest abundance. We show here that this largely reflects codon usage because heavily used codons were found in the highly abundant oligomers...

    journal_title:Nucleic acids research

    pub_type: 杂志文章

    doi:10.1093/nar/15.6.2627

    authors: Phillips GJ,Arnold J,Ivarie R

    更新日期:1987-03-25 00:00:00