A new detector for fully automatic peptide synthesis.

Abstract:

:A new method of monitoring the rate of reactions in solid-phase peptide synthesis is described. A conductivity detector in the reaction cell (patent applied for) enables the deprotection, washing and subsequent coupling stages to be examined in detail. The half-lives of the reactions can be calculated and hence the optimum reaction times can be predicted. Difficult sequences are sensed and appropriate action taken completely automatically.

journal_name

Biochem Soc Trans

authors

Fox JE

doi

10.1042/bst0181308a

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

1308-10

issue

6

eissn

0300-5127

issn

1470-8752

journal_volume

18

pub_type

杂志文章
  • CRISPR base editing applications for identifying cancer-driving mutations.

    abstract::CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage response. Given many cancers originate from poin...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST20200550

    authors: Pal M,Herold MJ

    更新日期:2021-01-15 00:00:00

  • Polyunsaturated fatty acid-specific elongation enzymes.

    abstract::We have isolated a novel gene (GLELO) from Mortierella alpina and its homologue (CEELO1) from Caenorhabditis elegans and demonstrate the involvement of their encoded proteins in the elongation of C(18) polyunsaturated fatty acids. ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:

    authors: Das T,Thurmond JM,Bobik E,Leonard AE,Parker-Barnes JM,Huang YS,Mukerji P

    更新日期:2000-12-01 00:00:00

  • Structural and functional comparison of 2-His-1-carboxylate and 3-His metallocentres in non-haem iron(II)-dependent enzymes.

    abstract::The canonical structural motif for co-ordination of non-haem ferrous iron in metal-dependent oxygenases is a facial triad of two histidine residues and one aspartate or glutamate residue. This so-called 2-His-1-carboxylate metallocentre is often accommodated in a double-stranded beta-helix fold with the iron-co-ordina...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0361180

    authors: Leitgeb S,Nidetzky B

    更新日期:2008-12-01 00:00:00

  • Chaperone-assisted assembly of the proteasome core particle.

    abstract::The 26S proteasome is a non-lysosomal protease in the cytosol and nucleus of eukaryotic cells. Its main function is to mediate ubiquitin-dependent proteolysis. The 26S proteasome is a multimeric complex composed by the 20S proteasome CP (core particle) and the 19S RPs (regulatory particles). Although the atomic struct...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0380029

    authors: Matias AC,Ramos PC,Dohmen RJ

    更新日期:2010-02-01 00:00:00

  • TRP channels activated by extracellular hypo-osmoticity in epithelia.

    abstract::TRP (transient receptor potential) channels comprise a superfamily of non-selective cation channels with at least seven subfamilies. The variety of subfamilies corresponds to the differences in the activation mechanisms and functions. TRPM3 (TRP melastatin 3) and TRPV4 (TRP vanilloid 3) have been characterized as cati...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0350091

    authors: Harteneck C,Reiter B

    更新日期:2007-02-01 00:00:00

  • Relating SMCHD1 structure to its function in epigenetic silencing.

    abstract::The structural maintenance of chromosomes hinge domain containing protein 1 (SMCHD1) is a large multidomain protein involved in epigenetic gene silencing. Variations in the SMCHD1 gene are associated with two debilitating human disorders, facioscapulohumeral muscular dystrophy (FSHD) and Bosma arhinia microphthalmia s...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST20200242

    authors: Gurzau AD,Blewitt ME,Czabotar PE,Murphy JM,Birkinshaw RW

    更新日期:2020-08-28 00:00:00

  • cAMP-specific phosphodiesterase-4D5 (PDE4D5) provides a paradigm for understanding the unique non-redundant roles that PDE4 isoforms play in shaping compartmentalized cAMP cell signalling.

    abstract::The PDE4 (phosphodiesterase-4) enzyme family consists of a distinct array of N-terminal splice variant isoforms arising from four subfamily genes (4A, 4B, 4C and 4D). These all hydrolyse specifically the intracellular second messenger cAMP. Although identical in catalytic function, each isoform appears to serve a non-...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0350938

    authors: Lynch MJ,Baillie GS,Houslay MD

    更新日期:2007-11-01 00:00:00

  • Physiological significance of L-amino acid sensing by extracellular Ca(2+)-sensing receptors.

    abstract::The calcium-sensing receptor is a multimodal, multimetabolic sensor that mediates the feedback-dependent control of whole body calcium metabolism. Remarkably, in addition to its role in Ca(2+)(o) (extracellular Ca(2+)) sensing, the CaR (Ca(2+)-sensing receptor) also responds to L-amino acids. L-amino acids appear to a...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0351195

    authors: Conigrave AD,Mun HC,Brennan SC

    更新日期:2007-11-01 00:00:00

  • Mesophyll conductance: the leaf corridors for photosynthesis.

    abstract::Besides stomata, the photosynthetic CO2 pathway also involves the transport of CO2 from the sub-stomatal air spaces inside to the carboxylation sites in the chloroplast stroma, where Rubisco is located. This pathway is far to be a simple and direct way, formed by series of consecutive barriers that the CO2 should cros...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST20190312

    authors: Gago J,Daloso DM,Carriquí M,Nadal M,Morales M,Araújo WL,Nunes-Nesi A,Flexas J

    更新日期:2020-04-29 00:00:00

  • Mitogenic stimulation of transcription by RNA polymerase III.

    abstract::Regulation of protein synthesis is an important aspect of growth control. RNA polymerase (pol) III plays a key role in this process by catalysing production of tRNA and 5 S rRNA. Growth factors trigger a rapid increase in pol III activity and this is essential for cell proliferation. The transcription factor TFIIIB pl...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0320976

    authors: Mauger E,Scott PH

    更新日期:2004-12-01 00:00:00

  • Lysosomal membrane proteins: life between acid and neutral conditions.

    abstract::Whereas we have a profound understanding about the function and biogenesis of the protein constituents in the lumen of the lysosomal compartment, much less is known about the functions of proteins of the lysosomal membrane. Proteomic analyses of the lysosomal membrane suggest that, apart from the well-known lysosomal ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0381420

    authors: Saftig P,Schröder B,Blanz J

    更新日期:2010-12-01 00:00:00

  • Glucose sensing via the protein kinase A pathway in Schizosaccharomyces pombe.

    abstract::The fission yeast Schizosaccharomyces pombe primarily detects glucose via a cAMP-signalling pathway. Components of this pathway include the Git3 G-protein-coupled receptor and a heterotrimeric G-protein, from which the Gpa2 Galpha subunit activates adenylate cyclase (Git2/Cyr1). Three additional proteins, Git1, Git7 a...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0330257

    authors: Hoffman CS

    更新日期:2005-02-01 00:00:00

  • EPR experiments to elucidate the structure of the ready and unready states of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F.

    abstract::Isolation and purification of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F under aerobic conditions leads to a mixture of two states, Ni-A (unready) and Ni-B (ready). The two states are distinguished by different activation times and different EPR spectra. HYSCORE and ENDOR data and DFT calculations sho...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0330007

    authors: van Gastel M,Fichtner C,Neese F,Lubitz W

    更新日期:2005-02-01 00:00:00

  • The role of TOG domains in microtubule plus end dynamics.

    abstract::The XMAP215 (Xenopus microtubule-associated protein 215) and CLASP [CLIP-170 (cytoskeletal linker protein 170) associated protein] microtubule plus end tracking families play central roles in the regulation of interphase microtubule dynamics and the proper formation of mitotic spindle architecture and flux. XMAP215 me...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0371002

    authors: Slep KC

    更新日期:2009-10-01 00:00:00

  • Mutation of genes controlling mRNA metabolism and protein synthesis predisposes to neurodevelopmental disorders.

    abstract::Brain development is a tightly controlled process that depends upon differentiation and function of neurons to allow for the formation of functional neural networks. Mutation of genes encoding structural proteins is well recognized as causal for neurodevelopmental disorders (NDDs). Recent studies have shown that aberr...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20150168

    authors: Sartor F,Anderson J,McCaig C,Miedzybrodzka Z,Müller B

    更新日期:2015-12-01 00:00:00

  • Colicin A binds to a novel binding site of TolA in the Escherichia coli periplasm.

    abstract::Colicins are protein antibiotics produced by Escherichia coli to kill closely related non-identical competing species. They have taken advantage of the promiscuity of several proteins in the cell envelope for entry into the bacterial cell. The Tol-Pal system comprises one such ensemble of periplasmic and membrane-asso...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20120239

    authors: Penfold CN,Li C,Zhang Y,Vankemmelbeke M,James R

    更新日期:2012-12-01 00:00:00

  • The tuberous sclerosis complex (TSC) pathway and mechanism of size control.

    abstract::We have identified three groups of growth-constraint genes using mosaic genetic screens in Drosophila melanogaster, including PTEN (phosphatase and tensin homologue deleted on chromosome 10), and the tuberous sclerosis complex (TSC) genes, Tsc1 and Tsc2. Our studies show that all three groups of genes participate in m...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/bst0310584

    authors: Potter CJ,Pedraza LG,Huang H,Xu T

    更新日期:2003-06-01 00:00:00

  • Regulation of chromosome dynamics by Hsk1/Cdc7 kinase.

    abstract::Hsk1 (homologue of Cdc7 kinase 1) of the fission yeast is a member of the conserved Cdc7 (cell division cycle 7) kinase family, and promotes initiation of chromosome replication by phosphorylating Mcm (minichromosome maintenance) subunits, essential components for the replicative helicase. Recent studies, however, ind...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20130217

    authors: Matsumoto S,Masai H

    更新日期:2013-12-01 00:00:00

  • Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast.

    abstract::We recently showed that transport of ergosterol from the ER (endoplasmic reticulum) to the sterol-enriched PM (plasma membrane) in yeast occurs by a non-vesicular (Sec18p-independent) mechanism that results in the equilibration of sterol pools in the two organelles [Baumann, Sullivan, Ohvo-Rekilä, Simonot, Pottekat, K...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0340356

    authors: Sullivan DP,Ohvo-Rekilä H,Baumann NA,Beh CT,Menon AK

    更新日期:2006-06-01 00:00:00

  • The petite purple photosynthetic powerpack.

    abstract::Photoreaction centres are Nature's solar batteries. These nanometre-scale power producers are responsible for transducing the energy of sunlight into a form that can be used by biological systems, thereby powering most of the biological activity on the planet. Although to the layman the word 'photosynthesis' is usuall...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0370400

    authors: Jones MR

    更新日期:2009-04-01 00:00:00

  • The Escherichia coli DNA translocase FtsK.

    abstract::Escherichia coli FtsK is a septum-located DNA translocase that co-ordinates the late stages of cytokinesis and chromosome segregation. Relatives of FtsK are present in most bacteria; in Bacillus subtilis, the FtsK orthologue, SpoIIIE, transfers the majority of a chromosome into the forespore during sporulation. DNA tr...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0380395

    authors: Sherratt DJ,Arciszewska LK,Crozat E,Graham JE,Grainge I

    更新日期:2010-04-01 00:00:00

  • Organization of endogenous clocks in insects.

    abstract::Insect and mammalian circadian clocks show striking similarities. They utilize homologous clock genes, generating self-sustained circadian oscillations in distinct master clocks of the brain, which then control rhythmic behaviour. The molecular mechanisms of rhythm generation were first uncovered in the fruit fly Dros...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20050957

    authors: Helfrich-Förster C

    更新日期:2005-11-01 00:00:00

  • Structural implications for the transformation of the Bacillus thuringiensis delta-endotoxins from water-soluble to membrane-inserted forms.

    abstract::Crystal structures combined with biochemical data show that the delta-endotoxins from Bacillus thuringiensis are structurally poised towards large-scale, irreversible conformational changes that transform them from the soluble protein bound at the cell surface into a membrane-embedded form causing lysis of susceptible...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/bst0290571

    authors: Li J,Derbyshire DJ,Promdonkoy B,Ellar DJ

    更新日期:2001-08-01 00:00:00

  • Immunoglobulin-binding domains: Protein L from Peptostreptococcus magnus.

    abstract::Protein L is a multidomain cell-wall protein isolated from Peptostreptococcus magnus. It belongs to a group of proteins that contain repeated domains that are able to bind to Igs without stimulating an immune response, the most characterized of this group being Protein A ( Staphylococcus aureus ) and Protein G ( Strep...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/bst0310716

    authors: Housden NG,Harrison S,Roberts SE,Beckingham JA,Graille M,Stura E,Gore MG

    更新日期:2003-06-01 00:00:00

  • Post-transcriptional control of mitochondrial protein composition in changing environmental conditions.

    abstract::In fluctuating environmental conditions, organisms must modulate their bioenergetic production in order to maintain cellular homeostasis for optimal fitness. Mitochondria are hubs for metabolite and energy generation. Mitochondria are also highly dynamic in their function: modulating their composition, size, density, ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST20200250

    authors: Tsuboi T,Leff J,Zid BM

    更新日期:2020-11-27 00:00:00

  • Understanding the yeast host cell response to recombinant membrane protein production.

    abstract::Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is typically achieved by synthesizing a protein of interest in host cells that can be cultured on a large scale, all...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0390719

    authors: Bawa Z,Bland CE,Bonander N,Bora N,Cartwright SP,Clare M,Conner MT,Darby RA,Dilworth MV,Holmes WJ,Jamshad M,Routledge SJ,Gross SR,Bill RM

    更新日期:2011-06-01 00:00:00

  • Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus.

    abstract::Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. In the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobu...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0370097

    authors: Wagner M,Berkner S,Ajon M,Driessen AJ,Lipps G,Albers SV

    更新日期:2009-02-01 00:00:00

  • Macrophage apoptosis in host immunity to mycobacterial infections.

    abstract::Macrophage apoptosis occurs within the granuloma, which is essential for successful immunity to tuberculosis. In vitro macrophage apoptosis is associated with the killing of intracellular Mycobacterium tuberculosis. A greater understanding of these observations will lead to new immunotherapies and improved vaccine des...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0320496

    authors: Fairbairn IP

    更新日期:2004-06-01 00:00:00

  • Regulation of alternative splicing by PTB and associated factors.

    abstract::PTB (polypyrimidine tract-binding protein) is a repressive regulator of alternative splicing. We have investigated the role of PTB in three model alternative splicing systems. In the alpha-actinin gene, PTB represses the SM (smooth muscle) exon by binding to key sites in the polypyrimidine tract. Repressive binding to...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0330457

    authors: Spellman R,Rideau A,Matlin A,Gooding C,Robinson F,McGlincy N,Grellscheid SN,Southby J,Wollerton M,Smith CW

    更新日期:2005-06-01 00:00:00

  • Applications of bacterial hydrogenases in waste decontamination, manufacture of novel bionanocatalysts and in sustainable energy.

    abstract::Bacterial hydrogenases have been harnessed to the removal of heavy metals from solution by reduction to less soluble metal species. For Pd(II), its bioreduction results in the deposition of cell-bound Pd(0)-nanoparticles that are ferromagnetic and have a high catalytic activity. Hydrogenases can also be used synthetic...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0330076

    authors: Macaskie LE,Baxter-Plant VS,Creamer NJ,Humphries AC,Mikheenko IP,Mikheenko PM,Penfold DW,Yong P

    更新日期:2005-02-01 00:00:00