Similarly derived and cultured hESC lines show variation in their developmental potential towards neuronal cells in long-term culture.

Abstract:

BACKGROUND:Human embryonic stem cells (hESCs) can differentiate into any human cell type, including CNS cells, and thus have high potential in regenerative medicine. Several protocols exist for neuronal differentiation of hESCs, which do not necessarily work for all hESC lines. MATERIALS & METHODS:We tested the differentiation capacity of four similarly derived and cultured hESC lines (HS181, HS360, HS362 and HS401) in suspension culture in relatively simple neural differentiation medium for up to 20 weeks. RESULTS:All the hESC lines differentiated into neuronal cells, but in a line-dependent manner. Using our method, the HS181- and HS360-derived neurospheres differentiated in vitro into pure neuronal cell populations within 6 weeks, whereas HS362 and HS401 reached their peak of differentiation in 12 weeks, but never produced pure neuronal cell populations using the present method. The withdrawal of FGF from suspension culture increased the in vitro differentiation potential. The hESC-derived neurospheres formed functional neuronal networks when replated on a microelectrode array and responded as expected to pharmacologic modulation. CONCLUSION:Simple neurosphere culture is a suitable method for producing hESC-derived neuronal cells that can form functional neuronal networks from a number of hESC lines. The variation in the differentiation potential of hESC lines into neuronal cells must be carefully considered by those comparing various differentiation methods and designing transplantation therapies for neuronal disorders.

journal_name

Regen Med

journal_title

Regenerative medicine

authors

Lappalainen RS,Salomäki M,Ylä-Outinen L,Heikkilä TJ,Hyttinen JA,Pihlajamäki H,Suuronen R,Skottman H,Narkilahti S

doi

10.2217/rme.10.58

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

749-62

issue

5

eissn

1746-0751

issn

1746-076X

journal_volume

5

pub_type

杂志文章
  • In vivo manipulation of stem cells for adipose tissue repair/reconstruction.

    abstract::Many features of adipose stem/progenitor cells, including their physiological functions and localization, have been clarified in the past decade. Adipose tissue turns over very slowly, with perivascular progenitor cells differentiating into new adipocytes to replace dead adipocytes. A number of clinical trials using f...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.2217/rme.11.62

    authors: Yoshimura K,Eto H,Kato H,Doi K,Aoi N

    更新日期:2011-11-01 00:00:00

  • Bioactive wound healing, bioaesthetics and biosurgery: three pillars of product development. Interview with Geoff MacKay.

    abstract::Tissue regeneration specialist company Organogenesis Inc. was one of the first biotech companies formed. Incorporated in 1985, the company was originally a spin-off from a research program at MIT. For the first 10-15 years, Organogenesis was heavily research based, but then gradually moved into development. The compan...

    journal_title:Regenerative medicine

    pub_type: 面试

    doi:10.2217/17460751.1.2.169

    authors: MacKay G

    更新日期:2006-03-01 00:00:00

  • Culture conditions for primary human limbal epithelial cells.

    abstract::The cornea at the front of the eye is covered by an epithelium. This epithelium is maintained by stem cells located at the periphery of the cornea, in a region known as the limbus. Because this region harbors the stem cells for the corneal epithelium, the so-called limbal stem cells, its culture provides considerable ...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme.09.7

    authors: Osei-Bempong C,Henein C,Ahmad S

    更新日期:2009-05-01 00:00:00

  • Fibrin hydrogels to deliver dental stem cells of the apical papilla for regenerative medicine.

    abstract:AIM:Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. METHODS:Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differe...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme.14.81

    authors: Germain L,De Berdt P,Vanacker J,Leprince J,Diogenes A,Jacobs D,Vandermeulen G,Bouzin C,Préat V,Dupont-Gillain C,des Rieux A

    更新日期:2015-01-01 00:00:00

  • Using poly(lactide-co-glycolide) electrospun scaffolds to deliver cultured epithelial cells to the cornea.

    abstract:AIMS:To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells. MATERIAL & METHODS:50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme.10.16

    authors: Deshpande P,McKean R,Blackwood KA,Senior RA,Ogunbanjo A,Ryan AJ,MacNeil S

    更新日期:2010-05-01 00:00:00

  • Regenerative medicine and hair loss: how hair follicle culture has advanced our understanding of treatment options for androgenetic alopecia.

    abstract::Many of the current drug therapies for androgenetic alopecia were discovered serendipitously, with hair growth observed as an off-target effect when drugs were used to treat a different disorder. Subsequently, several studies using cultured cells have enabled identification of hair growth modulators with similar prope...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.2217/rme.13.87

    authors: Higgins CA,Christiano AM

    更新日期:2014-01-01 00:00:00

  • Interview: stemming vision loss with stem cells: seeing is believing.

    abstract::Professor Coffey joined the faculty at the University of Oxford in 1987 on completing his doctoral work. In 1989, he was awarded a personal Royal Society Research Fellowship, at the same time moving to the University of Sheffield to establish a new laboratory for retinal transplantation. After 14 years at the Universi...

    journal_title:Regenerative medicine

    pub_type: 面试

    doi:10.2217/rme.09.33

    authors: Coffey P

    更新日期:2009-07-01 00:00:00

  • Repurposing nano-enabled polymeric scaffolds for tumor-wound management and 3D tumor engineering.

    abstract::The main challenges of cancer drugs are toxicity, effect on wound healing/patient outcome and in vivo instability. Polymeric scaffolds have been used separately for tissue regeneration in wound healing and as anticancer drug releasing devices. Bringing these two together in bifunctional scaffolds can provide a tool fo...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2020-0072

    authors: Goonoo N,Boodhun A,Ziman M,Gray E,Bhaw-Luximon A

    更新日期:2020-10-01 00:00:00

  • The regenerative therapies of the ankle degeneration: a focus on multipotential mesenchymal stromal cells.

    abstract::The ankle degeneration ranging from focal osteochondral lesions to osteoarthritis can cause a total joint function loss. With rising life expectancy and activity of the patients, various regenerative therapies were introduced aiming to preserve the joint function via the induction of cartilage and bone repair. Here, b...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2017-0104

    authors: El-Jawhari JJ,Brockett CL,Ktistakis I,Jones E,Giannoudis PV

    更新日期:2018-03-19 00:00:00

  • Safety and feasibility of intramyocardial versus intracoronary delivery of autologous cell therapy in advanced heart failure: the REGENERATE-IHD pilot study.

    abstract:AIM:This study presents an interim safety and feasibility analysis of the REGENERATE-IHD randomized controlled trial, which is examining the safety and efficacy of three different delivery routes of bone marrow-derived stem cells (BMSCs) in patients with ischemic heart failure. METHODS & RESULTS:The first 58 patients ...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.2217/rme.14.3

    authors: Mozid A,Yeo C,Arnous S,Ako E,Saunders N,Locca D,Brookman P,Archbold RA,Rothman M,Mills P,Agrawal S,Martin J,Mathur A

    更新日期:2014-05-01 00:00:00

  • Mesenchymal stem cells for vascular regeneration.

    abstract::Mesenchymal stem cells (MSCs) have tremendous potential for regenerative medicine, and have been researched for the treatment of cardiovascular diseases. MSCs are a promising cell type because of their ease of isolation and expansion, their multipotency and their low immunogenicity. However, in order to fully utilize ...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.2217/17460751.3.6.877

    authors: Huang NF,Li S

    更新日期:2008-11-01 00:00:00

  • New glossary of terms used in regenerative medicine: standardization continues to emerge as regenerative medicine matures.

    abstract::Regenerative medicine is an evolving, cross-disciplinary, international field that, as a result, uses terms that are either not widely understood, or may have a number of different meanings. Many stakeholders have identified this lack of clarity as a potential barrier to effective communication within the field. To ad...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme.09.34

    authors: Sheridan B,Harris N

    更新日期:2009-07-01 00:00:00

  • Sherley v. Sebelius.

    abstract::Following the reversal by the court of appeals of the injunction against federal funding of human embryonic stem cell research, Judge Lamberth has held such funding to be lawful and has dismissed the lawsuit. However, the litigation is likely to continue in the court of appeals and ultimately perhaps in the Supreme Co...

    journal_title:Regenerative medicine

    pub_type: 历史文章,杂志文章

    doi:10.2217/rme.11.70

    authors: Goldfarb N

    更新日期:2011-11-01 00:00:00

  • Pluripotent stem cell-derived pancreatic β-cells: potential for regenerative medicine in diabetes.

    abstract::Diabetes mellitus, which affects 346 million people, is one of the leading causes of death worldwide. Pancreatic β-cells, existing in the islets of Langerhans, play central roles in the progression of diabetes. An efficient strategy to produce functional pancreatic β-cells is important for both transplantation therapy...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.2217/rme.12.27

    authors: Kao DI,Chen S

    更新日期:2012-07-01 00:00:00

  • Reliable assessment of bone marrow and bone marrow concentrates using automated hematology analyzer.

    abstract::Aim: A limiting factor in advancement of bone marrow based cell therapies is the lack of characterization of cell products delivered to patients. Methods: Using an automated hematology analyzer that can be implemented in clinical setting, the composition of bone marrow aspirates (n = 17 patients) and bone marrow conce...

    journal_title:Regenerative medicine

    pub_type: 临床试验,杂志文章

    doi:10.2217/rme-2018-0173

    authors: Mantripragada VP,Piuzzi NS,George J,Bova W,Ng M,Boehm C,Muschler GF

    更新日期:2019-07-01 00:00:00

  • The past, present and future of ligament regenerative engineering.

    abstract::Regenerative engineering has been defined as the convergence of Advanced Materials Sciences, Stem Cell Sciences, Physics, Developmental Biology and Clinical Translation for the regeneration of complex tissues and organ systems. Anterior cruciate ligament (ACL) reconstruction necessitates the regeneration of bone, liga...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.2217/rme-2016-0125

    authors: Mengsteab PY,Nair LS,Laurencin CT

    更新日期:2016-12-01 00:00:00

  • Clinical use of autologous micro-fragmented fat progressively restores pain and function in shoulder osteoarthritis.

    abstract::Aim: We aim to show that the use of nondigested micro-fragmented adipose tissue (MFat™, Lipogems®) is a viable alternative for treatment of joint pain and inflammation associated dysfunction in shoulder osteoarthritis (OA). Materials & methods: A total of 25 subjects with OA received an injection of MFat™ and were fol...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2020-0069

    authors: Vinet-Jones H,F Darr K

    更新日期:2020-10-01 00:00:00

  • World Stem Cell Summit 2014. 3-5 December 2014, San Antonio, TX, USA.

    abstract::Among the many international conferences in the field of stem cells and regenerative medicine, WSCS is distinct in focusing its efforts to serve as the meeting point by multisector communities of research, clinics, industry, regulation, policy making and ethics. All are aiming at advancing stem cell innovation and new...

    journal_title:Regenerative medicine

    pub_type:

    doi:10.2217/rme.15.4

    authors: Hasegawa K,Asada T,Sengoku S,Nakatsuji N

    更新日期:2015-05-01 00:00:00

  • In vitro potential of human mesenchymal stem cells for corneal epithelial regeneration.

    abstract::Aim: To determine the potential of mesenchymal stem cells (MSC) for corneal epithelial regeneration in vitro. Materials & methods: Bone marrow MSC (BM-MSC) and adipose tissue MSC were analyzed for corneal epithelial and mesenchymal markers, using limbal stem cells and corneal cells as controls. MSC with better potenti...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2019-0067

    authors: Nieto-Nicolau N,Martín-Antonio B,Müller-Sánchez C,Casaroli-Marano RP

    更新日期:2020-03-01 00:00:00

  • Optimizing the safety of intradiscal platelet-rich plasma: an in vitro study with Cutibacterium acnes.

    abstract::Aim: The most common risk associated with intradiscal injection of platelet-rich plasma (PRP) is discitis with Cutibacterium acnes. It is hypothesized that antimicrobial activity of PRP can be enhanced through inclusion of leukocytes or antibiotics in the injectate. Materials & methods: Multiple PRP preparations of va...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2019-0098

    authors: Prysak MH,Lutz CG,Zukofsky TA,Katz JM,Everts PA,Lutz GE

    更新日期:2019-10-01 00:00:00

  • The UK Stem Cell Bank: a UK government-funded, international resource center for stem cell research.

    abstract::The UK Stem Cell Bank is a UK Research Council-funded initiative that aims to provide ethically sourced and quality controlled stocks of cells for researchers and also establish seed stocks of cell lines for clinical trials. Whilst the Bank is prohibited from carrying out basic stem cell research (to avoid conflicts o...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/17460751.1.1.139

    authors: Stacey G,Hunt CJ

    更新日期:2006-01-01 00:00:00

  • Endotoxin-induced silencing of mesoderm induction and functional differentiation: role of HMGB1 in pluripotency and infection.

    abstract:OBJECTIVES:Mechanisms underpinning Gram-negative bacterial vaginosis-induced birth anomalies are obscure. Ethical issues limit such studies on peri-implantation-stage human embryos. Here we have used embryoid bodies (EBs) as an in vitro model to examine the effect of Gram-negative bacterial endotoxins/lipopolysaccharid...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/17460751.3.1.23

    authors: Sivasubramaniyan K,Atluri RR,Sarda K,Arvind M,Balaji V,Deb KD

    更新日期:2008-01-01 00:00:00

  • Organization profile: genetics policy institute.

    abstract::Genetics Policy Institute (GPI) is a nonprofit, public interest organization recognized as a leading promoter and defender of stem cell research and other cutting-edge medical research targeting cures. Since GPI's inception in 2003, its mission has focused on establishing a positive policy, regulatory and societal fra...

    journal_title:Regenerative medicine

    pub_type: 新闻

    doi:10.2217/rme.09.6

    authors: Siegel B

    更新日期:2009-05-01 00:00:00

  • Identity and ownership issues in the regulation of autologous cells.

    abstract::Clinical application of autologous cells by businesses promoting unproven stem cell treatments represents the largest growth sector in this problematic industry, but also presents special challenges to regulators. Patients frequently identify autologous cells as personal property, using the language of 'ownership'. Th...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2017-0063

    authors: Sipp D

    更新日期:2017-10-01 00:00:00

  • Reparative medicine: from tissue engineering to joint surface regeneration.

    abstract::Biological regeneration is proving to be an increasingly attractive alternative/complement to prosthetic replacement of tissues and organs. In cell-based therapeutic approaches, cells are manipulated in vitro and administered to patients as living and dynamic biological agents. In this review, we have focused on the r...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/17460751.1.1.59

    authors: De Bari C,Pitzalis C,Dell'Accio F

    更新日期:2006-01-01 00:00:00

  • Short-term outcomes after pure bone marrow aspirate injection for severe knee osteoarthritis: a case series.

    abstract::Background: Bone marrow aspiration (BMA) is among a group of autologous cell-based therapies currently being explored to treat osteoarthritis (OA). Materials & methods: This retrospective case study observed ten patients (13 knees) with severe knee OA who failed extensive conservative treatment and were treated with B...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2019-0113

    authors: Colberg RE,Jurado Vélez JA,Walsh KP,Fleisig G

    更新日期:2020-07-01 00:00:00

  • Diabetes mellitus: an opportunity for therapy with stem cells?

    abstract::In both Type 1 and 2 diabetes, insufficient numbers of insulin-producing beta-cells are a major cause of defective control of blood glucose and its complications. Restoration of damaged beta-cells by endocrine pancreas regeneration would be an ideal therapeutic option. The possibility of generating insulin-secreting c...

    journal_title:Regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.2217/17460751.3.3.377

    authors: Sordi V,Bertuzzi F,Piemonti L

    更新日期:2008-05-01 00:00:00

  • Methods and practices to diversify cell-based products.

    abstract::Medicinal signaling cell (MSC)-based products represent emerging treatments in various therapeutic areas including cardiometabolic, inflammation, autoimmunity, orthopedics, wound healing and oncology. Exploring innovation beyond minimally manipulated plastic-adherent ex vivo expanded allogeneic MSCs enables product de...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/rme-2017-0093

    authors: Vertès AA

    更新日期:2017-12-01 00:00:00

  • Cognitive regeneration or enhancement: the ethical issues.

    abstract::The neurological and physiological basis of brain function and disease has been a significant focus of investigation throughout the history of medical research. Recent advances in understanding have led to the development of new treatments for diseases of the brain and defects of cognitive and behavioral function: pha...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/17460751.1.3.361

    authors: Chan S,Harris J

    更新日期:2006-05-01 00:00:00

  • A xenobiotic-free culture system for human limbal epithelial stem cells.

    abstract:AIMS:Murine 3T3 feeder cells are commonly used for stem cell expansion. Although 'feeder-free' systems are being developed for a variety of stem cells including embryonic, the use of feeder cells currently remains optimal for the expansion of epithelial stem cells. In this study, MRC-5, a human embryonic fibroblast cel...

    journal_title:Regenerative medicine

    pub_type: 杂志文章

    doi:10.2217/17460751.2.6.919

    authors: Notara M,Haddow DB,MacNeil S,Daniels JT

    更新日期:2007-11-01 00:00:00