Abstract:
:A new model of secondary and tertiary structure of higher plant 5S RNA is proposed. It consists of three helical domains: domain alpha includes stem I; domain beta contains stems II and III and loops B and C; domain gamma consists of stems IV and V and loops D and E. Except for, presumably, a canonical RNA-A like domain alpha, the two remaining domains apparently adopt a perturbed RNA-A structure due to irregularities within internal loops B and E and three bulges occurring in the model. Bending of RNA could bring loops B and E and/or C and D closer making tertiary interactions likely. The model differs from that suggested for eukaryotic 5S rRNA, by organization of domain gamma. Our model is based on the results of partial digestion obtained with single- and double-strand RNA specific nucleases. The proposed secondary structure is strongly supported by the observation that crude plant 5S rRNA contains abundant RNA, identified as domain gamma of 5S rRNA. Presumably it is excised from the 5S rRNA molecule by a specific nuclease present in lupin seeds. Experimental results were confirmed by computer-aided secondary structure prediction analysis of all higher plant 5S rRNAs. Differences observed between earlier proposed models and our proposition are discussed.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Joachimiak A,Nalaskowska M,Barciszewska M,Barciszewski J,Mashkova Tdoi
10.1016/0141-8130(90)90022-3subject
Has Abstractpub_date
1990-10-01 00:00:00pages
321-7issue
5eissn
0141-8130issn
1879-0003pii
0141-8130(90)90022-3journal_volume
12pub_type
杂志文章abstract::Water-soluble acrylamide (AAm)-grafted-chitosan (CS) copolymer (AAm-g-CS) was synthesized using potassium persulfate (PPS) initiator from which interpenetrating polymer network (IPN) microspheres were prepared by water-in-oil (w/o) emulsion that are cross-linked with glutaraldehyde (GA) and used for encapsulating nife...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.01.045
更新日期:2015-04-01 00:00:00
abstract::L-Lactic acid (LA)-grafted-gum arabic (GA) graft copolymer [OLLA-g-GA] was prepared by using microwave heating in which the hydrophilic GA was grafted with insitu grown lactic acid oligomer. The grafting at OH and NH groups in GA backbone with oligo-(l-lactic acid) (OLLA) chains proved with the help of FTIR and NMR an...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.07.199
更新日期:2018-12-01 00:00:00
abstract::In this work, we report on the preparation of a novel biodegradable textile scaffold made of palmitoyl-hyaluronan (palHA). Monofilament fibres of palHA with a diameter of 120μm were prepared by wet spinning. The wet-spun fibres were subsequently processed into a warp-knitted textile. To find a compromise between swell...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.10.076
更新日期:2017-02-01 00:00:00
abstract::Detailed characterization of enzyme susceptibility of bacterial cellulose containing N-acetylglucosamine (GlcNAc) residues (N-AcGBC) which possess high susceptibility for cellulase and lysozyme and slight susceptibility for chitinase was studied. Turbidimetric lysozyme assay of N-AcGBC showed that (i) the susceptibili...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(05)80076-5
更新日期:1992-12-01 00:00:00
abstract::In this study, fish gelatin and chitosan were used as the film-forming substrate, and different concentrations of TiO2-Ag were added to prepare composite films. The physicochemical characteristics and microstructure of the films were studied. The results showed that the addition of TiO2-Ag significantly increased the ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.070
更新日期:2020-07-01 00:00:00
abstract::The aim of this study was to investigate the inhibitory effects of molecular weight alteration of Dendrobium huoshanense polysaccharide on protein nonenzymatic glycation. For this purpose, one homogeneous active polysaccharide DHPD1 with molecular weight 3.2 kDa was extracted from D. huoshanense. GC analysis showed th...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.08.008
更新日期:2013-10-01 00:00:00
abstract::Effects of temperature and solvent condition on phase separation-induced molecular fractionation of gum arabic/hyaluronan (GA/HA) mixed solutions were investigated. Two gum arabic samples (EM10 and STD) with different molecular weights and polydispersity indices were used. Phase diagrams, including cloud and binodal c...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.05.073
更新日期:2018-09-01 00:00:00
abstract::Two diketone based oxovanadium complexes, viz., bis(4,4,4-trifluoro-1-phenylbutane-1,3-dionato)oxovanadium(IV) (1) and bis(1,1,1-trifluoropentane-2,4-dionato)oxovanadium(IV) (2), have been synthesized and characterized by spectroscopic and analytical techniques. The DNA binding and the cleaving ability of the complexe...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.02.027
更新日期:2015-05-01 00:00:00
abstract::Antarctic krill is a nutrient-rich crustacean that is one of the main species in the Antarctic ecosystem. Antarctic krill shell (AKS) can be used as raw materials to prepare chitin. In this study, lactic acid and dispase were used to prepare Antarctic krill chitin (AKC-1). Amino-monosaccharide contents of chitin sampl...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.244
更新日期:2020-12-01 00:00:00
abstract::Extracellular alkaline protease producing salt tolerant alkaliphilic actinobacteria, Nocardiopsis xinjiangensis strain OM-6 was isolated from Okha Madi (OM) site of coastal Gujarat, India. The purified protease was stable even at 70°C in 100mM Ca2+ with Kd=20×10-3 and t1/2=34min. The activation energies (E), enthalpy ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.02.157
更新日期:2018-07-01 00:00:00
abstract::Recently, rare sugars have caused extensively attention due to their beneficial physiological functions and potential applications in food systems and medical fields. Ketose 3-epimerase (KEase) can catalyze reversibly the epimerization between ketoses which is the pivotal enzyme in Izumoring strategy and an effective ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.112
更新日期:2019-10-01 00:00:00
abstract::The aim of this study was to explore the therapeutic effect and underling mechanism of Dendrobium officinale polysaccharides (DOPS) on two well-established animal models of learning and memory disabilities. Model of estrogen deficiency caused learning and memory disability can be induced by ovariectomy in mice, and mi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.230
更新日期:2019-04-01 00:00:00
abstract::The development of thermosensitive smart hydrogels with suitable thermosensitivity is of great importance for various biomedical and pharmaceutical applications. In this study, thermosensitive chitosan/carboxymethylcellulose/scleroglucan/montmorillonite (CHT/CMC/SGL/MMT) nanocomposite hydrogels were prepared for biome...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.087
更新日期:2020-11-01 00:00:00
abstract::Despite impressive progress of macromolecular encapsulation technique based on hyperbranched polymer (HP), the use of natural HP for guest encapsulation has rarely been reported. Herein, we present the simple synthesis of amphiphilic PGP-DC from natural peach gum polysaccharide (PGP) and demonstrate that the PGP-DC ca...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.03.151
更新日期:2020-07-01 00:00:00
abstract::The endogenous deposition of protein aggregates with fibrillar morphology is known to govern the genesis of several neurodisorders. The investigation of naturally occurring small molecules which can restrict the fibrillation process can help in the design of rational therapeutics for neurodegenerative diseases. We rep...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.08.006
更新日期:2013-10-01 00:00:00
abstract::With the increasing demand for simple, efficient, environmentally friendly preparation methods to produce cellulose nanofibers for reinforcing a biodegradable film is increased, the role of nanofibers from the pure cellulose produced by bacteria becomes more important. This work characterized bacterial cellulose nanof...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.05.178
更新日期:2019-08-15 00:00:00
abstract::The present study is first of its kind that focuses upon the extraction of bromelain from pineapple core waste and stabilising it as insoluble cross-linked aggregates. The influence of process variables such as the choice of precipitant, type of cross-linker, concentration of cross-linker and the reaction time for cro...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.04.220
更新日期:2020-04-28 00:00:00
abstract::The aim of this research work was to investigate novel tools given by nanotechnology and green chemistry for improving the disadvantages typically associated to the starch-based films: water susceptibility and brittle mechanical behavior. With this in mind, four food packaging film systems were developed from corn sta...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2021.01.048
更新日期:2021-01-13 00:00:00
abstract::Electrospinning technique was used to fabricate chloroamphenicol loaded poly (L-lactic acid)/ZrO2 (CP-PLLA/ZrO2) nanofibrous membranes. The average diameter of drug carrier PLLA/ZrO2 nanofibers increased with ZrO2 concentration increasing and ribbon-shape fibers were present when ZrO2 content reached 3% and above. The...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.10.002
更新日期:2013-11-01 00:00:00
abstract::α-Glucosidase is a critical associated enzyme with type 2 diabetes mellitus in humans. Inhibition of α-glucosidase is important due to the potential effect of down regulating glucose absorption in patients. In this study, the inhibitory activity of flavone luteolin on α-glucosidase and their interaction mechanism were...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.12.007
更新日期:2014-03-01 00:00:00
abstract::Complex coacervation was achieved by mixing the lysing enzyme from T. harzianum (LYS) with polyallylamine hydrochloride (PAH). We show in this work that the study electrostatic complexes conformation can lead to the formation of dense complexes. We systematically investigated the effects of pH and the mass ratio on th...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.266
更新日期:2019-03-01 00:00:00
abstract::Curdlan is a polysaccharide that consists of β-1,3-linked glucose residues. A polysaccharide-producing bacterium isolated from soil samples was identified as Pseudomonas sp. QL212. The polysaccharide was purified to homogeneity via sequential ethanol precipitation, deproteinization, CM ion-exchange, and gel chromatogr...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.04.027
更新日期:2016-08-01 00:00:00
abstract::Sequences of 40 very diverse representatives of the alpha-crystallin-small heat-shock protein (alpha-Hsp) superfamily are compared. Their characteristic C-terminal 'alpha-crystallin domain' of 80-100 residues contains short consensus sequences that are highly conserved from prokaryotes to eukaryotes. There are, in add...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/s0141-8130(98)00013-0
更新日期:1998-05-01 00:00:00
abstract::This work aims at investigating Fe3O4-coated nanofibers based on cellulose acetate (CA) and chitosan (CS) for adsorption of Cr(VI), Ni(II), and phenol from aqueous solutions. The synthesized Fe3O4 nanoparticles (NPs), and the modified nanofibrous adsorbents are characterized by using FTIR and FE-SEM analyses. The opti...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.058
更新日期:2020-07-01 00:00:00
abstract::An efficient method was developed to prepare oxidized potato starch under the pasting point by Electro-Fenton system, in which a complex of sodium citrate and Fe2+ (SC-Fe2+) was used as catalyst, and H2O2 was used as oxidant. In the preparation process the oxidation degree of starch was controlled by the molar ratios ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.10.012
更新日期:2019-01-01 00:00:00
abstract::N-acetylglucosaminidase produced from Lecanicillium lecanii on submerged culture displayed hydrolytic and transglycosylation activities. The highest specific activity for the enzyme was 1.87 U/mg after 120 h of culture. The chromatographic purification for a single protein fraction showed a molecular weight of 50.4 kD...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.12.237
更新日期:2020-02-15 00:00:00
abstract::Naked barley grains were germinated at 25 °C for 12, 24, and 36 h, followed by hot air and infrared drying. Changes in structural, physicochemical, and functional properties of naked barley flour and starch after germination and drying were evaluated. Germinated and dried flour showed loose structure in starch granule...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.10.114
更新日期:2020-12-15 00:00:00
abstract::In recent years, an enormous number of naturally occurring biological macromolecules has been reported worldwide due to its antibacterial and anticancerous potential. Among them, in this study, the copper containing respiratory protein namely haemocyanin (HC) was isolated from the haemolymph of mud crab Scylla serrata...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.228
更新日期:2020-12-01 00:00:00
abstract::The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions. The interaction between b...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.03.079
更新日期:2018-07-15 00:00:00
abstract::Purification, characterization and hypoglycemic activities of polysaccharide from Lycium barbarum L. were investigated in this study. A water soluble polysaccharide (LBP) was obtained with ultrafiltration membranes separation, which was further purified by chromatography of DEAE cellulose column and Sephadex G-150 to ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.03.026
更新日期:2015-01-01 00:00:00